Isolation and characterization of proteoglycans from the swarm rat chondrosarcoma.
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Proteoglycan monomer (D1) and aggregate (A1) preparations were isolated from 4 M guanidinium chloride extracts of the Swarm rat chondrosarcoma and contained only small proteoglycan fragments, indicating that extensive enzymatic degradation had occurred.About:
This article is published in Journal of Biological Chemistry.The article was published on 1975-08-10 and is currently open access. It has received 543 citations till now. The article focuses on the topics: Proteoglycan & Benzamidine.read more
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Sequence‐Specific Antiproliferative Effects of Antisense and End‐Capping‐Modified Antisense Oligodeoxynucleotides Targeted against the 5′‐Terminus of Basic‐Fibroblast‐Growth‐Factor mRNA in Coronary Smooth Muscle Cells
Annette Schmidt,Jiirgen Sindermann,Anusch Peyman,Eugen Uhlmann,David William Will,Joachim G. Müller,Giinter Breithardt,Eckhart Buddecke +7 more
TL;DR: The results point to possible new therapeutic strategies for the use of antisense methodology in the suppression of post-angioplasty restenosis and an effective and specific antiproliferative potency of bFGF-specific antisense oligonucleotides.
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Chondrolysis of the hip joint in a patient receiving long-term hemodialysis: histologic and biochemical evaluation.
TL;DR: Biochemical studies of articular cartilage retained at surgery revealed deposition of exclusively small proteoglycans in the matrix, which may have been an important factor leading to the rapid chondrolysis in this patient.
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Morphological analysis of abnormal digital chondrogenesis in the brachypod (bpH) mouse limb in organ culture.
TL;DR: Using this in vitro system, the proximal-to-distal effect on the severity of cartilage reduction was intensified in the brachypod explants with an intermediate expression in the heterozygotes, and a deficiency in and an abnormal distribution of the proteoglycans were revealed.
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Collagen fibril ultrastructure alters after glycanolytic digestion.
TL;DR: Fibril rearrangement after digestion may be explained in terms of the wrap of matrix proteoglycans around each fibril, which could make "mid" collagen fibrils free to regress into "thin" as well as to merge together into "thick" fibrILS.
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Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
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The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis
Klaus Weber,Mary Osborn +1 more
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Automatic recording apparatus for use in the chromatography of amino acids.
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A modified uronic acid carbazole reaction
T. Bitter,Helen Muir +1 more
TL;DR: It has been found possible to distinguish betweenHeparin, heparin derivatives, and other polyuronides of connective tissue by comparing the effect of chlorides on the color yield in both procedures by modifying Dische's carbazole reaction for uronic acid in the presence of borate.
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Enzymatic Methods for the Determination of Small Quantities of Isomeric Chondroitin Sulfates
TL;DR: Micromethods have been developed and 35S-labeled chondroitin sulfates A, B, and C in a given mixture have been precisely and rapidly determined by measuring radioactivity alone.