Isolation and characterization of proteoglycans from the swarm rat chondrosarcoma.
Reads0
Chats0
TLDR
Proteoglycan monomer (D1) and aggregate (A1) preparations were isolated from 4 M guanidinium chloride extracts of the Swarm rat chondrosarcoma and contained only small proteoglycan fragments, indicating that extensive enzymatic degradation had occurred.About:
This article is published in Journal of Biological Chemistry.The article was published on 1975-08-10 and is currently open access. It has received 543 citations till now. The article focuses on the topics: Proteoglycan & Benzamidine.read more
Citations
More filters
Journal ArticleDOI
Topographic localization of a 116,000-dalton protein in cartilage.
TL;DR: The 116-kD subunit protein appears to be a ubiquitous matrix protein in cartilage and was located throughout the matrix in specimens of normal adult articular cartilage from several species.
Journal ArticleDOI
Human arthritic synovial fluid influences proteoglycan biosynthesis and degradation in organ culture of bovine nasal cartilage.
TL;DR: The influence of synovial fluid and serum from patients with inflammatory joint disease on proteoglycan metabolism was studied in organ culture of bovine nasal cartilage and the findings are consistent with the presence in synovIAL fluid of mediators stimulating the chondrocytes both to activate proteoglycans degradation and to reduce proteogly can biosynthesis.
Journal ArticleDOI
The ultrastructure of cultures from the Swarm rat chondrosarcoma.
TL;DR: This rapid synthesis of new matrix, together with the biochemical and morphological characterization of the proteoglycans as typical of cartilage, demonstrates the value of Swarm rat chondrosarcoma cultures as a model system for studies of synthesis, secretion, and organization of extracellular matrix.
Journal ArticleDOI
Matrix metalloproteinases are active following guanidine hydrochloride extraction of cartilage: generation of DIPEN neoepitope during dialysis.
Heather Stanton,Amanda J. Fosang +1 more
TL;DR: It is reported that DIPEN(341) neoepitope can be generated post-culture, by dialysing GuHCl(1)-denatured samples against unbuffered, deionized water at 4 degrees C and it is shown that EDTA must be included in the GuH Cl extractant, as well as the dialysis buffer, in order to block post- culture processing of aggrecan by MMPs.
Journal Article
Immunochemical and biochemical comparisons between embryonic chick bone marrow and epiphyseal cartilage chondroitin/dermatan sulphate proteoglycans
TL;DR: Both marrow and cartilage were similar in that they contained, as their major components, large, aggregating proteoglycans and link proteins that were immunochemically and biochemically identical.
References
More filters
Journal Article
Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
Journal ArticleDOI
The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis
Klaus Weber,Mary Osborn +1 more
TL;DR: The results show that the polyacrylamide gel electrophoresis method can be used with great confidence to determine the molecular weights of polypeptide chains for a wide variety of proteins.
Journal ArticleDOI
Automatic recording apparatus for use in the chromatography of amino acids.
Journal ArticleDOI
A modified uronic acid carbazole reaction
T. Bitter,Helen Muir +1 more
TL;DR: It has been found possible to distinguish betweenHeparin, heparin derivatives, and other polyuronides of connective tissue by comparing the effect of chlorides on the color yield in both procedures by modifying Dische's carbazole reaction for uronic acid in the presence of borate.
Journal ArticleDOI
Enzymatic Methods for the Determination of Small Quantities of Isomeric Chondroitin Sulfates
TL;DR: Micromethods have been developed and 35S-labeled chondroitin sulfates A, B, and C in a given mixture have been precisely and rapidly determined by measuring radioactivity alone.