Isolation and characterization of proteoglycans from the swarm rat chondrosarcoma.
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Proteoglycan monomer (D1) and aggregate (A1) preparations were isolated from 4 M guanidinium chloride extracts of the Swarm rat chondrosarcoma and contained only small proteoglycan fragments, indicating that extensive enzymatic degradation had occurred.About:
This article is published in Journal of Biological Chemistry.The article was published on 1975-08-10 and is currently open access. It has received 543 citations till now. The article focuses on the topics: Proteoglycan & Benzamidine.read more
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The distribution of proteoglycans of high electrophoretic mobility in cartilages from different species and of different ages
Victor Stanescu,R. Stanescu +1 more
TL;DR: The distribution of small proteoglycans of high relative electrophoretic mobility in cartilage of various species and of different ages was studied and a rapid migrating population was found in articular cartilages of young humans, baboons, calves, pigs, rabbits, rats, chickens and in mandibular and vertebral cartilage of dog-fish.
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Proteoglycan aggregates in adult human costal cartilage.
TL;DR: Proteoglycans extracted by isotonic solutions are relatively small and may be in vivo degradation products of whole molecules, but to a much lesser extent than found in other cartilages like bovine nasal or porcine epiglottal cartilage.
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β-d-Xyloside-mediated alteration in the synthesis of basement membrane proteoglycan
TL;DR: The data indicate that xyloside can substitute for the heparan sulfate initiation site on the core protein of the proteoglycan and that this initiation is enhanced in the absence of core protein.
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Cell fractions from rat rib growth cartilage: Biochemical characterization of matrix molecules☆
TL;DR: In an attempt to isolate and characterize the putative target cells for growth hormone, chondrocytes were isolated from rat rib growth cartilage and fractionated by centrifugation in a discontinuous Percoll gradient, which resulted in three cell fractions with differing properties.
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Immunohistochemical localization of collagenous proteins in cartilaginous tumors: characteristic distribution of type IX collagen.
Atsuhiro Kawashima,Yoshimichi Ueda,Hiroyuki Tsuchiya,Katsuro Tomita,Yutaka Nagai,Isao Nakanishi +5 more
TL;DR: It is suggested that neoplastic cartilage is characterized initially by an uneven distribution of type IX collagen, prior to any alteration of other types of collagen; the diverse expressions of intercellular components in cartilaginous tumors may be one indicator for malignancy.
References
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Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
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The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis
Klaus Weber,Mary Osborn +1 more
TL;DR: The results show that the polyacrylamide gel electrophoresis method can be used with great confidence to determine the molecular weights of polypeptide chains for a wide variety of proteins.
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Automatic recording apparatus for use in the chromatography of amino acids.
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A modified uronic acid carbazole reaction
T. Bitter,Helen Muir +1 more
TL;DR: It has been found possible to distinguish betweenHeparin, heparin derivatives, and other polyuronides of connective tissue by comparing the effect of chlorides on the color yield in both procedures by modifying Dische's carbazole reaction for uronic acid in the presence of borate.
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Enzymatic Methods for the Determination of Small Quantities of Isomeric Chondroitin Sulfates
TL;DR: Micromethods have been developed and 35S-labeled chondroitin sulfates A, B, and C in a given mixture have been precisely and rapidly determined by measuring radioactivity alone.