Isolation and characterization of proteoglycans from the swarm rat chondrosarcoma.
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Proteoglycan monomer (D1) and aggregate (A1) preparations were isolated from 4 M guanidinium chloride extracts of the Swarm rat chondrosarcoma and contained only small proteoglycan fragments, indicating that extensive enzymatic degradation had occurred.About:
This article is published in Journal of Biological Chemistry.The article was published on 1975-08-10 and is currently open access. It has received 543 citations till now. The article focuses on the topics: Proteoglycan & Benzamidine.read more
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Chemical and physical changes in proteoglycans during development of chick limb bud chondrocytes grown in vitro.
Journal ArticleDOI
Spatial and temporal changes in the distribution of proteoglycans during avian neural crest development
Roberto Perris,Danuta Krotoski,Thomas Lallier,Carmen R. Domingo,J.M. Sorrell,Marianne Bronner-Fraser +5 more
TL;DR: In general, chondroitin/keratan sulfate proteoglycans are abundant in regions where neural crest cells are absent, and their segmental distribution inversely correlates with that of neural crest-derived ganglia.
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Biosynthesis of heparan sulfate proteoglycan by human colon carcinoma cells and its localization at the cell surface.
TL;DR: The results indicate that colon carcinoma cells synthesize HS-PGs with distinct structural and metabolic characteristics: a large secretory pool with high turnover, which appears to be synthesized as an integral membrane component and localized primarily at the cell surface, and a small nonsecretory Pool with low turnover localized predominantly within the cell interior.
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Characterization and biosynthesis of proteoglycans of corneal stroma from Rhesus monkey
TL;DR: The analyses indicate that the proteoglycans synthesized by corneas in organ culture were similar to, if not identical with, their counterparts in the stroma although they are synthesized in different prportions in vitro than they acumulate in vivo.
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The effect of compressive force applied to the intervertebral disc in vivo. A study of proteoglycans and collagen.
William C. Hutton,Yasumitsu Toribatake,William A. Elmer,Timothy Ganey,Katsuro Tomita,Thomas E. Whitesides +5 more
TL;DR: A strong correlation between increases in force and force‐weeks (force multiplied by number of weeks) and increases in collagen type I accompanied by decreases in proteoglycans, chondroitin sulfate, and collagen type II for both experimental discs as compared with corresponding values in the controls.
References
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Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
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The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis
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TL;DR: It has been found possible to distinguish betweenHeparin, heparin derivatives, and other polyuronides of connective tissue by comparing the effect of chlorides on the color yield in both procedures by modifying Dische's carbazole reaction for uronic acid in the presence of borate.
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Enzymatic Methods for the Determination of Small Quantities of Isomeric Chondroitin Sulfates
TL;DR: Micromethods have been developed and 35S-labeled chondroitin sulfates A, B, and C in a given mixture have been precisely and rapidly determined by measuring radioactivity alone.