Isolation and characterization of proteoglycans from the swarm rat chondrosarcoma.
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Proteoglycan monomer (D1) and aggregate (A1) preparations were isolated from 4 M guanidinium chloride extracts of the Swarm rat chondrosarcoma and contained only small proteoglycan fragments, indicating that extensive enzymatic degradation had occurred.About:
This article is published in Journal of Biological Chemistry.The article was published on 1975-08-10 and is currently open access. It has received 543 citations till now. The article focuses on the topics: Proteoglycan & Benzamidine.read more
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Size distribution of cartilage proteoglycans determined by sedimentation field flow fractionation
TL;DR: Sedimentation field flow fractionation (SFFF) as mentioned in this paper is a high-resolution separation method for cartilage proteoglycan subunits and aggregates with recoveries of greater than 80%.
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Proteoglycans (Glycosaminoglycans/Mucopolysaccharides)
TL;DR: This book discusses the linkage of GAG to Core Protein, and the characterization of Proteoglycans through biosynthesis and characterization, as well as some of the mechanisms used to extract GAGs.
Journal ArticleDOI
Light-scattering studies of chick limb bud proteoglycan aggregate.
R. L. Shogren,John Blackwell,Alex M. Jamieson,David A. Carrino,David G. Pechak,Arnold I. Caplan +5 more
TL;DR: In this article, static and dynamic light-scattering methods have been used to investigate the structure of chick limb bud chondrocyte proteoglycan aggregate in 0.4 M guanidinium chloride.
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Inhibition of the proteolytic contaminant in commercial xanthine oxidase preparations by serum protein fractions
TL;DR: An easily prepared fraction of fetal calf serum was found to inhibit virtually all of the proteolytic contaminant without affecting superoxide generation, and the effects attainable with the "purified" enzyme were demonstrated with two protease sensitive targets.
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Isolation of arterial wall proteoglycans
TL;DR: Proteoglycans of the arterial media-intima were extracted with associative and dissociative salt solutions and showed a glycosaminoglycan distribution pattern similar to that for the total tissue.
References
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Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
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The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis
Klaus Weber,Mary Osborn +1 more
TL;DR: The results show that the polyacrylamide gel electrophoresis method can be used with great confidence to determine the molecular weights of polypeptide chains for a wide variety of proteins.
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Automatic recording apparatus for use in the chromatography of amino acids.
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A modified uronic acid carbazole reaction
T. Bitter,Helen Muir +1 more
TL;DR: It has been found possible to distinguish betweenHeparin, heparin derivatives, and other polyuronides of connective tissue by comparing the effect of chlorides on the color yield in both procedures by modifying Dische's carbazole reaction for uronic acid in the presence of borate.
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Enzymatic Methods for the Determination of Small Quantities of Isomeric Chondroitin Sulfates
TL;DR: Micromethods have been developed and 35S-labeled chondroitin sulfates A, B, and C in a given mixture have been precisely and rapidly determined by measuring radioactivity alone.