Isolation and characterization of proteoglycans from the swarm rat chondrosarcoma.
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Proteoglycan monomer (D1) and aggregate (A1) preparations were isolated from 4 M guanidinium chloride extracts of the Swarm rat chondrosarcoma and contained only small proteoglycan fragments, indicating that extensive enzymatic degradation had occurred.About:
This article is published in Journal of Biological Chemistry.The article was published on 1975-08-10 and is currently open access. It has received 543 citations till now. The article focuses on the topics: Proteoglycan & Benzamidine.read more
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In situ interaction of cartilage proteoglycans with matrix proteins.
Alexis J. Aletras,C.P. Tsiganos +1 more
TL;DR: The interaction of proteoglycans with other matrix proteins via thiol-disulphide interchange was explored and larger proteogly cans had a size and composition similar to those of aggregating proteoglyCans.
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Binding of calcium by proteoglycans in vitro
TL;DR: It was found that some of the preparations do contain small amounts of calcium but these amounts are insufficient to saturate the binding sites potentially available to this end, and it is suggested that the proteoglycans inhibit the formation of calcium phosphate precipitates in vitro not because the calcium is site bound but because thecium ions are territorially bound.
Journal ArticleDOI
Basic Fibroblast Growth Factor Controls the Expression and Molecular Structure of Heparan Sulfate in Corneal Endothelial Cells
TL;DR: A bFGF-dependent change in the rate of synthesis and structural features of the membrane-associated heparan sulfate in corneal endothelial cells is suggested, which could influence its bF GF-binding and antiproliferative activity.
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Selective aggregation of proteoglycans with hyaluronic acid.
TL;DR: Chromatography on Sepharose 2B under dissociative conditions demonstrated that the nonaggregated N1 monomer is intermediate in size between the disaggregated monomers AH1-D1 and AH 1-D2.
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Analysis of perinotochordal materials. I. Studies on proteoglycans synthesis.
TL;DR: The results of the present study indicate that the proteoglycan extracted from the 8 day old notochord culture consists of predominantly small proteoglycans, while the large aggregates form less than 30% of the total.
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Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
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The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis
Klaus Weber,Mary Osborn +1 more
TL;DR: The results show that the polyacrylamide gel electrophoresis method can be used with great confidence to determine the molecular weights of polypeptide chains for a wide variety of proteins.
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Automatic recording apparatus for use in the chromatography of amino acids.
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A modified uronic acid carbazole reaction
T. Bitter,Helen Muir +1 more
TL;DR: It has been found possible to distinguish betweenHeparin, heparin derivatives, and other polyuronides of connective tissue by comparing the effect of chlorides on the color yield in both procedures by modifying Dische's carbazole reaction for uronic acid in the presence of borate.
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Enzymatic Methods for the Determination of Small Quantities of Isomeric Chondroitin Sulfates
TL;DR: Micromethods have been developed and 35S-labeled chondroitin sulfates A, B, and C in a given mixture have been precisely and rapidly determined by measuring radioactivity alone.