Isolation and characterization of proteoglycans from the swarm rat chondrosarcoma.
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Proteoglycan monomer (D1) and aggregate (A1) preparations were isolated from 4 M guanidinium chloride extracts of the Swarm rat chondrosarcoma and contained only small proteoglycan fragments, indicating that extensive enzymatic degradation had occurred.About:
This article is published in Journal of Biological Chemistry.The article was published on 1975-08-10 and is currently open access. It has received 543 citations till now. The article focuses on the topics: Proteoglycan & Benzamidine.read more
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Occurrence and structural characterization of versican-like proteoglycan in human vitreous.
TL;DR: It was found that apart from significant amounts of collagen, hyaluronan and sialylated glycoproteins, the human vitreous gel also contained low amounts of versican-like proteoglycan, which is able to bind lectins via its C-terminal region and may bridge or interconnect various constituents of the extracellular matrix via its terminal domains in order to stabilize large supramolecular complexes at the Vitreous.
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Proteoglycans from the cartilage of young hammerhead shark Sphyrna lewini
TL;DR: The data suggest that the proteoglycans in shark fin cartilage present a structural organization which is different from that of mammalian cartilages, as well as the ability to form complexes with hyaluronic acid.
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Ultracentrifugal characterization of proteoglycans from rat growth cartilage.
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Purification of keratan sulfate proteoglycan from monkey cornea.
TL;DR: Structural analyses of the linkage region oligosaccharides and the intact oligOSaccharide-peptides suggest that the intact keratan sulfate proteoglycans contain an average of about one intact oligosACcharide per keratan sulphate linkage site.
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Chemical and immunological characterization of proteoglycans of embryonic chick calvaria.
TL;DR: The results of double immunodiffusion studies and radioimmune inhibition assays indicate that chick calvarium contains proteoglycans with at least one species-specific antigenic determinant which cross-reacts with antiserum against chick cartilage proteoglycan subunits.
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Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
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The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis
Klaus Weber,Mary Osborn +1 more
TL;DR: The results show that the polyacrylamide gel electrophoresis method can be used with great confidence to determine the molecular weights of polypeptide chains for a wide variety of proteins.
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Automatic recording apparatus for use in the chromatography of amino acids.
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A modified uronic acid carbazole reaction
T. Bitter,Helen Muir +1 more
TL;DR: It has been found possible to distinguish betweenHeparin, heparin derivatives, and other polyuronides of connective tissue by comparing the effect of chlorides on the color yield in both procedures by modifying Dische's carbazole reaction for uronic acid in the presence of borate.
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Enzymatic Methods for the Determination of Small Quantities of Isomeric Chondroitin Sulfates
TL;DR: Micromethods have been developed and 35S-labeled chondroitin sulfates A, B, and C in a given mixture have been precisely and rapidly determined by measuring radioactivity alone.