Isolation and characterization of proteoglycans from the swarm rat chondrosarcoma.
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Proteoglycan monomer (D1) and aggregate (A1) preparations were isolated from 4 M guanidinium chloride extracts of the Swarm rat chondrosarcoma and contained only small proteoglycan fragments, indicating that extensive enzymatic degradation had occurred.About:
This article is published in Journal of Biological Chemistry.The article was published on 1975-08-10 and is currently open access. It has received 543 citations till now. The article focuses on the topics: Proteoglycan & Benzamidine.read more
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Differentiation of coronary smooth muscle cells to a cell cycle-arrested hypertrophic growth status by a synthetic non-toxic heparin-mimicking compound.
TL;DR: It appears that RG-13,577 induces a phenotype transformation of coronary SMC into a metabolically active hypertrophic status that could promote repair processes after balloon angioplasty (PTCA) without stimulating cell proliferation.
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Proteogylcan heterogeneity in embryonic chick articular and epiphyseal cartilages.
Nagaswamisri Vasan,James W. Lash +1 more
TL;DR: Proteoglycans from two regions of the chick limb rudiment (articular and epiphyseal) were examined for chemical microheterogeneity, finding differences are consonant with the hypothesis that extracellular matrix components may be structurally adapted to a tissue's developmental fate or function.
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Proteoglycan synthesis by skeletal muscle undergoing bone matrix-directed transformation into cartilage in vitro.
TL;DR: Material of lower molecular size, from cultures grown on demineralized bone, bind to exogenous HA, whereas the elution pattern of sulfated material from control cultures remains essentially unchanged, suggesting that proteoglycans of low hydrodynamic size may participate in the early formation of proteoglycan aggregate.
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Microinjury to the synovial membrane may cause disaggregation of proteoglycans in rabbit knee joint articular cartilage.
Linda Frost,Peter Ghosh +1 more
TL;DR: Proteoglycans isolated from articular cartilage of mature rabbits subjected to two or more consecutive intraarticular injections of sterile saline 24 h apart showed an aggregation defect in the presence of excess hyaluronic acid (HA).
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Novel biochemical pathways for 5-Fluorouracil in managing experimental hepatocellular carcinoma in rats.
TL;DR: It is possible that proteolytic activity, expressed as upregulated pepsin and free cathepsin-D activities, is increased in HCC, accompanied by extracellular matrix macromolecular disturbance, manifested as decreased TGAGs, collagen and TSA, with marked increase in FGA liver tissue content.
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Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
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The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis
Klaus Weber,Mary Osborn +1 more
TL;DR: The results show that the polyacrylamide gel electrophoresis method can be used with great confidence to determine the molecular weights of polypeptide chains for a wide variety of proteins.
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Automatic recording apparatus for use in the chromatography of amino acids.
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A modified uronic acid carbazole reaction
T. Bitter,Helen Muir +1 more
TL;DR: It has been found possible to distinguish betweenHeparin, heparin derivatives, and other polyuronides of connective tissue by comparing the effect of chlorides on the color yield in both procedures by modifying Dische's carbazole reaction for uronic acid in the presence of borate.
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Enzymatic Methods for the Determination of Small Quantities of Isomeric Chondroitin Sulfates
TL;DR: Micromethods have been developed and 35S-labeled chondroitin sulfates A, B, and C in a given mixture have been precisely and rapidly determined by measuring radioactivity alone.