Journal ArticleDOI
Negligible Particle-Specific Antibacterial Activity of Silver Nanoparticles
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TLDR
This work suggests that AgNP morphological properties known to affect antimicrobial activity are indirect effectors that primarily influence Ag(+) release, and antibacterial activity could be controlled by modulating Ag(+ release, possibly through manipulation of oxygen availability, particle size, shape, and/or type of coating.Abstract:
For nearly a decade, researchers have debated the mechanisms by which AgNPs exert toxicity to bacteria and other organisms. The most elusive question has been whether the AgNPs exert direct “particle-specific” effects beyond the known antimicrobial activity of released silver ions (Ag+). Here, we infer that Ag+ is the definitive molecular toxicant. We rule out direct particle-specific biological effects by showing the lack of toxicity of AgNPs when synthesized and tested under strictly anaerobic conditions that preclude Ag(0) oxidation and Ag+ release. Furthermore, we demonstrate that the toxicity of various AgNPs (PEG- or PVP- coated, of three different sizes each) accurately follows the dose–response pattern of E. coli exposed to Ag+ (added as AgNO3). Surprisingly, E. coli survival was stimulated by relatively low (sublethal) concentration of all tested AgNPs and AgNO3 (at 3–8 μg/L Ag+, or 12–31% of the minimum lethal concentration (MLC)), suggesting a hormetic response that would be counterproductive t...read more
Citations
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Cubic nano-silver-decorated manganese dioxide micromotors: enhanced propulsion and antibacterial performance
TL;DR: The continuous movement of micromotors, corresponding to violent mass transfer, along with the on-the-fly release of silver ions, greatly enhanced bacteria killing efficacy, with about 14% increase in bacterial death in 0.2% H2O2 solution as compared to no motors.
Journal ArticleDOI
Development of silver nanoparticle-based hydrogel composites for antimicrobial activity
TL;DR: This work presents a simple strategy for producing Ag NPs-containing hydrogel based on natural alginate polymers, which prevents the direct release ofAg NPs, while allowing the release of Ag+ cations out of the hydrogels.
Journal ArticleDOI
Miniaturized liquid chromatography coupled on-line to in-tube solid-phase microextraction for characterization of metallic nanoparticles using plasmonic measurements. A tutorial.
TL;DR: This tutorial aims at providing guidelines for analyzing metallic nanoparticles (NPs) and their dispersions by using methods based on miniaturized liquid chromatography with diode array detection (MinLC-DAD) and coupled on-line to in-tube solid-phase microextraction (IT-SPME).
Journal ArticleDOI
Silver nanoparticles in complex media: an easy procedure to discriminate between metallic silver nanoparticles, reprecipitated silver chloride, and dissolved silver species
Kateryna Loza,Matthias Epple +1 more
TL;DR: In this paper, a chemical separation procedure was proposed to quantify silver, silver chloride, and dissolved silver species after immersion into a typical cell culture medium (DMEM + 10% FCS).
Journal ArticleDOI
Efficient intracellular delivery and improved biocompatibility of colloidal silver nanoparticles towards intracellular SERS immuno-sensing
TL;DR: The TATHA2 facilitated approach efficiently delivered high payload with no toxicity, pre-requisites for intracellular applications of plasmonic metal nanoparticles (PMNPs) in sensing and therapeutics.
References
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Journal ArticleDOI
The bactericidal effect of silver nanoparticles
Jose Ruben Morones,Jose Luis Elechiguerra,A. Camacho,Katherine B. Holt,Juan B. Kouri,Jose Tapia Ramirez,Miguel Jose Yacaman +6 more
TL;DR: The results indicate that the bactericidal properties of the nanoparticles are size dependent, since the only nanoparticles that present a direct interaction with the bacteria preferentially have a diameter of approximately 1-10 nm.
Journal ArticleDOI
Antimicrobial effects of silver nanoparticles
Jun Sung Kim,Eunye Kuk,Kyeong Nam Yu,Jong-Ho Kim,Sungjin Park,Hu Jang Lee,So Hyun Kim,Young Kyung Park,Yong Ho Park,Cheol Yong Hwang,Yong-Kwon Kim,Yoon-Sik Lee,Dae Hong Jeong,Myung-Haing Cho +13 more
TL;DR: The results suggest that Ag nanoparticles can be used as effective growth inhibitors in various microorganisms, making them applicable to diverse medical devices and antimicrobial control systems.
Journal ArticleDOI
Does the antibacterial activity of silver nanoparticles depend on the shape of the nanoparticle? A study of the Gram-negative bacterium Escherichia coli.
TL;DR: This is the first comparative study on the bactericidal properties of silver nanoparticles of different shapes, and the results demonstrate thatsilver nanoparticles undergo a shape-dependent interaction with the gram-negative organism E. coli.
Journal ArticleDOI
Cytotoxicity and Genotoxicity of Silver Nanoparticles in Human Cells
TL;DR: A possible mechanism of toxicity is proposed which involves disruption of the mitochondrial respiratory chain by Ag-np leading to production of ROS and interruption of ATP synthesis, which in turn cause DNA damage.
Journal ArticleDOI
Silver Colloid Nanoparticles: Synthesis, Characterization, and Their Antibacterial Activity
Aleš Panáček,Libor Kvítek,Robert Prucek,Milan Kolar,Renata Vecerova,N. Pizúrová,Virender K. Sharma,Tat’jana Nevečná,Radek Zboril +8 more
TL;DR: The reduction of [Ag(NH(3))(2)](+) by maltose produced silver particles with a narrow size distribution with an average size of 25 nm, which showed high antimicrobial and bactericidal activity against Gram-positive and Gram-negative bacteria, including highly multiresistant strains such as methicillin-resistant Staphylococcus aureus.
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