Journal ArticleDOI
Negligible Particle-Specific Antibacterial Activity of Silver Nanoparticles
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TLDR
This work suggests that AgNP morphological properties known to affect antimicrobial activity are indirect effectors that primarily influence Ag(+) release, and antibacterial activity could be controlled by modulating Ag(+ release, possibly through manipulation of oxygen availability, particle size, shape, and/or type of coating.Abstract:
For nearly a decade, researchers have debated the mechanisms by which AgNPs exert toxicity to bacteria and other organisms. The most elusive question has been whether the AgNPs exert direct “particle-specific” effects beyond the known antimicrobial activity of released silver ions (Ag+). Here, we infer that Ag+ is the definitive molecular toxicant. We rule out direct particle-specific biological effects by showing the lack of toxicity of AgNPs when synthesized and tested under strictly anaerobic conditions that preclude Ag(0) oxidation and Ag+ release. Furthermore, we demonstrate that the toxicity of various AgNPs (PEG- or PVP- coated, of three different sizes each) accurately follows the dose–response pattern of E. coli exposed to Ag+ (added as AgNO3). Surprisingly, E. coli survival was stimulated by relatively low (sublethal) concentration of all tested AgNPs and AgNO3 (at 3–8 μg/L Ag+, or 12–31% of the minimum lethal concentration (MLC)), suggesting a hormetic response that would be counterproductive t...read more
Citations
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Journal ArticleDOI
Attenuation of Microbial Stress Due to Nano-Ag and Nano-TiO2 Interactions under Dark Conditions.
TL;DR: The importance of probing the toxicity of ENM mixtures under environmental conditions to assess how chemical interactions between nanoparticles change the toxicological effects of single ENMs in unexpected ways is emphasized.
Journal ArticleDOI
Detrimental impact of silica nanoparticles on the nanomechanical properties of Escherichia coli , studied by AFM
Marion Mathelié-Guinlet,Christine Grauby-Heywang,Axel Martin,Hugo Février,Fabien Moroté,Alexandre Vilquin,Laure Béven,Marie-Hélène Delville,Touria Cohen-Bouhacina +8 more
TL;DR: In this paper, the impact of nanoparticles on the mechanical properties of E. coli cells after their exposure, and relating it to their toxic activity under a critical diameter was investigated, and the subsequent toxic behavior was finally confirmed by the presence of membrane residues, due to cell lysis, exhibiting typical adhesion features.
Journal ArticleDOI
Silver Nanoparticle Synthesis Using Monosaccharides and Their Growth Inhibitory Activity against Gram-Negative and Positive Bacteria
TL;DR: Observations suggested that the growth inhibition of Ag NPs is mediated by interfering with the bacterial cell wall peptidoglycan, which is attributed to the higher aggregation rate for larger NPs on cell wall.
Journal ArticleDOI
Bactericidal effects and accelerated wound healing using Tb 4 O 7 nanoparticles with intrinsic oxidase-like activity
Chen Li,Yurong Sun,Xiaoping Li,Sanhong Fan,Yimin Liu,Xiumei Jiang,Mary D. Boudreau,Yue Pan,Xin Tian,Jun-Jie Yin +9 more
TL;DR: It is shown that Tb4O7 NPs have intrinsic oxidase-like activity and show effective antibacterial ability both in vitro and in vivo and may have a potential application in wound healing.
Journal ArticleDOI
Centrifugation-based assay for examining nanoparticle–lipid membrane binding and disruption
Aihong Xi,Geoffrey D. Bothun +1 more
TL;DR: The centrifugation assay provides a rapid and straightforward way to screen nanoparticle-membrane interactions as a function of nanoparticle surface functionalization, membrane lipid composition, and monovalent salt concentration.
References
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Journal ArticleDOI
The bactericidal effect of silver nanoparticles
Jose Ruben Morones,Jose Luis Elechiguerra,A. Camacho,Katherine B. Holt,Juan B. Kouri,Jose Tapia Ramirez,Miguel Jose Yacaman +6 more
TL;DR: The results indicate that the bactericidal properties of the nanoparticles are size dependent, since the only nanoparticles that present a direct interaction with the bacteria preferentially have a diameter of approximately 1-10 nm.
Journal ArticleDOI
Antimicrobial effects of silver nanoparticles
Jun Sung Kim,Eunye Kuk,Kyeong Nam Yu,Jong-Ho Kim,Sungjin Park,Hu Jang Lee,So Hyun Kim,Young Kyung Park,Yong Ho Park,Cheol Yong Hwang,Yong-Kwon Kim,Yoon-Sik Lee,Dae Hong Jeong,Myung-Haing Cho +13 more
TL;DR: The results suggest that Ag nanoparticles can be used as effective growth inhibitors in various microorganisms, making them applicable to diverse medical devices and antimicrobial control systems.
Journal ArticleDOI
Does the antibacterial activity of silver nanoparticles depend on the shape of the nanoparticle? A study of the Gram-negative bacterium Escherichia coli.
TL;DR: This is the first comparative study on the bactericidal properties of silver nanoparticles of different shapes, and the results demonstrate thatsilver nanoparticles undergo a shape-dependent interaction with the gram-negative organism E. coli.
Journal ArticleDOI
Cytotoxicity and Genotoxicity of Silver Nanoparticles in Human Cells
TL;DR: A possible mechanism of toxicity is proposed which involves disruption of the mitochondrial respiratory chain by Ag-np leading to production of ROS and interruption of ATP synthesis, which in turn cause DNA damage.
Journal ArticleDOI
Silver Colloid Nanoparticles: Synthesis, Characterization, and Their Antibacterial Activity
Aleš Panáček,Libor Kvítek,Robert Prucek,Milan Kolar,Renata Vecerova,N. Pizúrová,Virender K. Sharma,Tat’jana Nevečná,Radek Zboril +8 more
TL;DR: The reduction of [Ag(NH(3))(2)](+) by maltose produced silver particles with a narrow size distribution with an average size of 25 nm, which showed high antimicrobial and bactericidal activity against Gram-positive and Gram-negative bacteria, including highly multiresistant strains such as methicillin-resistant Staphylococcus aureus.
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