Nucleic acid-based approaches to investigate microbial-related cheese quality defects
Daniel J. O'Sullivan,Linda Giblin,Paul L.H. McSweeney,Jeremiah J. Sheehan,Paul D. Cotter,Paul D. Cotter +5 more
TLDR
The DNA-based methods that are available to detect/quantify spoilage bacteria, and relevant metabolic pathways in cheeses are reviewed and it is highlighted how these strategies can be employed to improve cheese quality and reduce the associated economic burden on cheese processors.Abstract:
The microbial profile of cheese is a primary determinant of cheese quality. Microorganisms can contribute to aroma and taste defects, form biogenic amines, cause gas and secondary fermentation defects, and can contribute to cheese pinking and mineral deposition issues. These defects may be as a result of seasonality and the variability in the composition of the milk supplied, variations in cheese processing parameters, as well as the nature and number of the non-starter microorganisms which come from the milk or other environmental sources. Such defects can be responsible for production and product recall costs and thus represent a significant economic burden for the dairy industry worldwide. Traditional non-molecular approaches are often considered biased and have inherently slow turnaround times. Molecular techniques can provide early and rapid detection of defects that result from the presence of specific spoilage microbes and, ultimately, assist in enhancing cheese quality and reducing costs. Here we review the DNA-based methods that are available to detect/quantify spoilage bacteria, and relevant metabolic pathways in cheeses and, in the process, highlight how these strategies can be employed to improve cheese quality and reduce the associated economic burden on cheese processors.read more
Citations
More filters
Journal ArticleDOI
Antibiotic resistance and drug modification: Synthesis, characterization and bioactivity of newly modified potent ciprofloxacin derivatives.
TL;DR: In this paper, the authors developed new derivatives of commercial antibiotics using different organic reagents and tested these derivatives against different microorganisms are the main goals of the study, which reveals that most of the modified drugs show high to moderate antibacterial activity.
Journal ArticleDOI
Antifungal activity of a synthetic human β-defensin 3 and potential applications in cereal-based products
Thibaut Thery,James C. Tharappel,Joanna Kraszewska,Michael C. Beckett,Ursula Bond,Elke K. Arendt +5 more
TL;DR: It is shown that the linear analogue of HBD-3 displays antifungal activity against food contaminants in vitro and can be used to extend the shelf-life of bread.
Journal ArticleDOI
Changing paradigm of antibiotic resistance amongst Escherichia coli isolates in Indian pediatric population.
Taru Singh,Praveen Kumar Singh,Sajad Ahmad Dar,Sajad Ahmad Dar,Shafiul Haque,Naseem Akhter,Shukla Das +6 more
TL;DR: The presence of antibiotic resistant genes in E. coli isolates from healthy children indicate the development, dissemination and carriage of antibiotic resistance in their gut, and suggest the implementation of active surveillance and stewardship programs to promote appropriate antibiotic use and minimizing further danger.
Journal ArticleDOI
The Influence of Sogatella furcifera (Hemiptera: Delphacidae) Migratory Events on the Southern Rice Black-Streaked Dwarf Virus Epidemics.
Yan Wu,Guo Zhang,Xiao Chen,Xi-Jie Li,Kai Xiong,Shu-Pei Cao,Yan-Yue Hu,Ming-Hong Lu,Wan-Cai Liu,Hoang-Anh Tuan,Guo-Jun Qi,Bao-Ping Zhai +11 more
TL;DR: The relationship between SRBSDV dynamics and migration of S. furcifera populations via an in-depth analysis of meteorological background was revealed, and it was shown that Northern Vietnam was the direct virus source area of theSRBSDV infection in China, and South Central Coast of Vietnamwas the original source area.
Journal ArticleDOI
Bodies of the Anthropocene: on the interactive plasticity of earth systems and biological organisms
TL;DR: The Anthropocene literature has brought attention to the plasticity and porosity of Earth systems under the dramatic impact of human activities as discussed by the authors, and the authors in this paper focus on the plasticities and porosities of Earth system under the effects of human activity.
References
More filters
Journal ArticleDOI
Next-Generation DNA Sequencing Methods
TL;DR: An astounding potential exists for next-generation DNA sequencing technologies to bring enormous change in genetic and biological research and to enhance the authors' fundamental biological knowledge.
Journal ArticleDOI
Impact of 16S rRNA Gene Sequence Analysis for Identification of Bacteria on Clinical Microbiology and Infectious Diseases
TL;DR: A future challenge is to translate information from 16S rRNA gene sequencing into convenient biochemical testing schemes, making the accuracy of the genotypic identification available to the smaller and routine clinical microbiology laboratories.
Journal ArticleDOI
Lactic acid bacteria as functional starter cultures for the food fermentation industry
Frédéric Leroy,Luc De Vuyst +1 more
TL;DR: New starter cultures of lactic acid bacteria with an industrially important functionality are being developed that can contribute to the microbial safety or offer one or more organoleptic, technological, nutritional, or health advantages.
Journal ArticleDOI
The real-time polymerase chain reaction
Mikael Kubista,José Manuel Andrade,Martin Bengtsson,Amin Forootan,Jiri Jonák,Kristina Lind,Radek Sindelka,Robert Sjöback,Björn Sjögreen,Linda Strömbom,Anders Ståhlberg,Neven Zoric +11 more
TL;DR: The scientific, medical, and diagnostic communities have been presented the most powerful tool for quantitative nucleic acids analysis: real-time PCR, a refinement of the original Polymerase Chain Reaction (PCR) developed by Kary Mullis and coworkers in the mid 80:ies.
Journal ArticleDOI
Biogenic amines: their importance in foods
TL;DR: A better knowledge of the factors controlling the formation of amines is necessary in order to improve the quality and safety of food as discussed by the authors, which can be found in both raw and processed foods.
Related Papers (5)
QIIME allows analysis of high-throughput community sequencing data.
J. Gregory Caporaso,Justin Kuczynski,Jesse Stombaugh,Kyle Bittinger,Frederic D. Bushman,Elizabeth K. Costello,Noah Fierer,Antonio Gonzalez Peña,Julia K. Goodrich,Jeffrey I. Gordon,Gavin A. Huttley,Scott T. Kelley,Dan Knights,Jeremy E. Koenig,Ruth E. Ley,Catherine A. Lozupone,Daniel McDonald,Brian D. Muegge,Meg Pirrung,Jens Reeder,Joel Sevinsky,Peter J. Turnbaugh,William A. Walters,Jeremy Widmann,Tanya Yatsunenko,Jesse R. Zaneveld,Rob Knight,Rob Knight +27 more
Introducing mothur: Open-Source, Platform-Independent, Community-Supported Software for Describing and Comparing Microbial Communities
Patrick D. Schloss,Patrick D. Schloss,Sarah L. Westcott,Sarah L. Westcott,Thomas Ryabin,Justine R. Hall,Martin Hartmann,Emily B. Hollister,Ryan A. Lesniewski,Brian B. Oakley,Donovan H. Parks,Courtney J. Robinson,Jason W. Sahl,Blaz Stres,Gerhard G. Thallinger,David J. Van Horn,Carolyn F. Weber +16 more