Nucleic acid-based approaches to investigate microbial-related cheese quality defects
Daniel J. O'Sullivan,Linda Giblin,Paul L.H. McSweeney,Jeremiah J. Sheehan,Paul D. Cotter,Paul D. Cotter +5 more
TLDR
The DNA-based methods that are available to detect/quantify spoilage bacteria, and relevant metabolic pathways in cheeses are reviewed and it is highlighted how these strategies can be employed to improve cheese quality and reduce the associated economic burden on cheese processors.Abstract:
The microbial profile of cheese is a primary determinant of cheese quality. Microorganisms can contribute to aroma and taste defects, form biogenic amines, cause gas and secondary fermentation defects, and can contribute to cheese pinking and mineral deposition issues. These defects may be as a result of seasonality and the variability in the composition of the milk supplied, variations in cheese processing parameters, as well as the nature and number of the non-starter microorganisms which come from the milk or other environmental sources. Such defects can be responsible for production and product recall costs and thus represent a significant economic burden for the dairy industry worldwide. Traditional non-molecular approaches are often considered biased and have inherently slow turnaround times. Molecular techniques can provide early and rapid detection of defects that result from the presence of specific spoilage microbes and, ultimately, assist in enhancing cheese quality and reducing costs. Here we review the DNA-based methods that are available to detect/quantify spoilage bacteria, and relevant metabolic pathways in cheeses and, in the process, highlight how these strategies can be employed to improve cheese quality and reduce the associated economic burden on cheese processors.read more
Citations
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Bacterial communities differ between plant species and soil type, and differentially influence seedling establishment on serpentine soils
TL;DR: While soil microbial community composition influenced seedling survival in the current study, further study is required to disentangle the role of microbial associations and plant tolerance to serpentine.
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Effect of supplementation of fermented milk drink containing probiotic Lactobacillus casei Shirota on the concentrations of aflatoxin biomarkers among employees of Universiti Putra Malaysia: a randomised, double-blind, cross-over, placebo-controlled study.
Sabran Mohd Redzwan,Mohd Sokhini Abd Mutalib,Jia-Sheng Wang,Zuraini Ahmad,Min-Su Kang,Nurul 'Aqilah Abdul Rahman,Elham Nikbakht Nasrabadi,Rosita Jamaluddin +7 more
TL;DR: A longer intervention study is warranted to investigate the effects of continuous LcS consumption to prevent dietary aflatoxin exposure and to determine the effectiveness of a fermented milk drink containing Lactobacillus casei Shirota (LcS).
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In vitro antimicrobial potential of extracts and phytoconstituents from Gymnema sylvestre R.Br. leaves and their biosafety evaluation
Daljit Singh Arora,Henna Sood +1 more
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Protease Activities Triggered by Ralstonia solanacearum Infection in Susceptible and Tolerant Tomato Lines.
Marc Planas-Marquès,Marc Planas-Marquès,Marti Bernardo-Faura,Judith K Paulus,Farnusch Kaschani,Markus Kaiser,Marc Valls,Marc Valls,Renier A. L. van der Hoorn,Núria S. Coll +9 more
TL;DR: The finding that deeper changes in network topology take place in the susceptible tomato variety, suggesting that the tolerant cultivar might be more prepared to face R. solanacearum, denotes the importance of novel proteomic approaches such as ABPP to provide new insights on old and elusive questions regarding the molecular basis of resistance to R. solvent.
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New synergistic co-culture of Corylus avellana cells and Epicoccum nigrum for paclitaxel production
TL;DR: It is shown that co-culture of C. avellana cells and E. nigrum surpassed monocultures in terms of paclitaxel production designating their synergistic interaction potential.
References
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Journal ArticleDOI
Next-Generation DNA Sequencing Methods
TL;DR: An astounding potential exists for next-generation DNA sequencing technologies to bring enormous change in genetic and biological research and to enhance the authors' fundamental biological knowledge.
Journal ArticleDOI
Impact of 16S rRNA Gene Sequence Analysis for Identification of Bacteria on Clinical Microbiology and Infectious Diseases
TL;DR: A future challenge is to translate information from 16S rRNA gene sequencing into convenient biochemical testing schemes, making the accuracy of the genotypic identification available to the smaller and routine clinical microbiology laboratories.
Journal ArticleDOI
Lactic acid bacteria as functional starter cultures for the food fermentation industry
Frédéric Leroy,Luc De Vuyst +1 more
TL;DR: New starter cultures of lactic acid bacteria with an industrially important functionality are being developed that can contribute to the microbial safety or offer one or more organoleptic, technological, nutritional, or health advantages.
Journal ArticleDOI
The real-time polymerase chain reaction
Mikael Kubista,José Manuel Andrade,Martin Bengtsson,Amin Forootan,Jiri Jonák,Kristina Lind,Radek Sindelka,Robert Sjöback,Björn Sjögreen,Linda Strömbom,Anders Ståhlberg,Neven Zoric +11 more
TL;DR: The scientific, medical, and diagnostic communities have been presented the most powerful tool for quantitative nucleic acids analysis: real-time PCR, a refinement of the original Polymerase Chain Reaction (PCR) developed by Kary Mullis and coworkers in the mid 80:ies.
Journal ArticleDOI
Biogenic amines: their importance in foods
TL;DR: A better knowledge of the factors controlling the formation of amines is necessary in order to improve the quality and safety of food as discussed by the authors, which can be found in both raw and processed foods.
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