Nucleic acid-based approaches to investigate microbial-related cheese quality defects
Daniel J. O'Sullivan,Linda Giblin,Paul L.H. McSweeney,Jeremiah J. Sheehan,Paul D. Cotter,Paul D. Cotter +5 more
TLDR
The DNA-based methods that are available to detect/quantify spoilage bacteria, and relevant metabolic pathways in cheeses are reviewed and it is highlighted how these strategies can be employed to improve cheese quality and reduce the associated economic burden on cheese processors.Abstract:
The microbial profile of cheese is a primary determinant of cheese quality. Microorganisms can contribute to aroma and taste defects, form biogenic amines, cause gas and secondary fermentation defects, and can contribute to cheese pinking and mineral deposition issues. These defects may be as a result of seasonality and the variability in the composition of the milk supplied, variations in cheese processing parameters, as well as the nature and number of the non-starter microorganisms which come from the milk or other environmental sources. Such defects can be responsible for production and product recall costs and thus represent a significant economic burden for the dairy industry worldwide. Traditional non-molecular approaches are often considered biased and have inherently slow turnaround times. Molecular techniques can provide early and rapid detection of defects that result from the presence of specific spoilage microbes and, ultimately, assist in enhancing cheese quality and reducing costs. Here we review the DNA-based methods that are available to detect/quantify spoilage bacteria, and relevant metabolic pathways in cheeses and, in the process, highlight how these strategies can be employed to improve cheese quality and reduce the associated economic burden on cheese processors.read more
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Shifting gear in antimicrobial and anticancer peptides biophysical studies: from vesicles to cells
TL;DR: Biophysical studies on the mechanism of action of antimicrobial and anticancer peptides performed directly on cells are reviewed to correlate vesicle‐based and cell‐based studies and fill the vesicles‐cell interdisciplinary gap.
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The archaeal lipidome in estuarine sediment dominated by members of the Miscellaneous Crenarchaeotal Group
Travis B. Meador,Marshall W. Bowles,Cassandre Sara Lazar,Cassandre Sara Lazar,Chun Zhu,Andreas P Teske,Kai-Uwe Hinrichs +6 more
TL;DR: The recently identified butanetriol dibiphytanyl glycerol tetraethers (BDGT), which increased relatively to other lipids with depth, were correlated with the relative abundance of MCG in archaeal 16S rRNA clone libraries, and were depleted throughout the depth profile, suggesting BDGT lipids as putative biomarkers of an MCG community that may either be autotrophic or feeding on organic substrates transported by porewater.
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Biofilm forming Salmonella strains exhibit enhanced thermal resistance in wheat flour
Rossana Villa-Rojas,Mei-Jun Zhu,Narayan C. Paul,Peter Gray,Jie Xu,Devendra H. Shah,Juming Tang +6 more
TL;DR: In this paper, the authors investigated the influence of biofilms on thermal resistance of Salmonella enteritidis in low-moisture foods and found that the biofilm forming ability was not associated with resistance to desiccation.
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Investigation of Antimicrobial Resistance in Escherichia coli and Enterococci Isolated from Tibetan Pigs
Peng Li,Dongfang Wu,Kunyao Liu,Sizhu Suolang,Tao He,Xuan Liu,Congming Wu,Yang Wang,Degui Lin +8 more
TL;DR: E. coli and Enterococcus isolated from free-ranging Tibetan pigs showed relatively lower resistance rates than those in other areas of China, where more intensive farming practices are used, and revealed that free-range husbandry and absence of antibiotic use could decrease the occurrence of antimicrobial resistance to some extent.
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Developments in taxol production through endophytic fungal biotechnology: a review
TL;DR: Improved fermentation techniques and different biotechnological strategies such as gene cloning, gene transformation, mutations are widely being used on endophytic fungi in order to increase the productivity of the taxol.
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Mikael Kubista,José Manuel Andrade,Martin Bengtsson,Amin Forootan,Jiri Jonák,Kristina Lind,Radek Sindelka,Robert Sjöback,Björn Sjögreen,Linda Strömbom,Anders Ståhlberg,Neven Zoric +11 more
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