Nucleic acid-based approaches to investigate microbial-related cheese quality defects
Daniel J. O'Sullivan,Linda Giblin,Paul L.H. McSweeney,Jeremiah J. Sheehan,Paul D. Cotter,Paul D. Cotter +5 more
TLDR
The DNA-based methods that are available to detect/quantify spoilage bacteria, and relevant metabolic pathways in cheeses are reviewed and it is highlighted how these strategies can be employed to improve cheese quality and reduce the associated economic burden on cheese processors.Abstract:
The microbial profile of cheese is a primary determinant of cheese quality. Microorganisms can contribute to aroma and taste defects, form biogenic amines, cause gas and secondary fermentation defects, and can contribute to cheese pinking and mineral deposition issues. These defects may be as a result of seasonality and the variability in the composition of the milk supplied, variations in cheese processing parameters, as well as the nature and number of the non-starter microorganisms which come from the milk or other environmental sources. Such defects can be responsible for production and product recall costs and thus represent a significant economic burden for the dairy industry worldwide. Traditional non-molecular approaches are often considered biased and have inherently slow turnaround times. Molecular techniques can provide early and rapid detection of defects that result from the presence of specific spoilage microbes and, ultimately, assist in enhancing cheese quality and reducing costs. Here we review the DNA-based methods that are available to detect/quantify spoilage bacteria, and relevant metabolic pathways in cheeses and, in the process, highlight how these strategies can be employed to improve cheese quality and reduce the associated economic burden on cheese processors.read more
Citations
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Antimicrobial peptide resistance of Vibrio cholerae results from an LPS modification pathway related to nonribosomal peptide synthetases
Jeremy C. Henderson,Christopher D. Fage,Joe R. Cannon,Jennifer S. Brodbelt,Adrian T. Keatinge-Clay,M. Stephen Trent +5 more
TL;DR: The mechanistic determination of initial steps in the AlmEFG pathway is presented and it is verified that AlmF is an aminoacyl carrier protein and AlmE as the enzyme required to activate AlmF as a functional carrier protein.
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A single regulatory gene is sufficient to alter Vibrio aestuarianus pathogenicity in oysters
David Goudenège,David Goudenège,David Goudenège,Marie-Agnès Travers,Astrid Lemire,Astrid Lemire,Astrid Lemire,Bruno Petton,Philippe Haffner,Yannick Labreuche,Yannick Labreuche,Yannick Labreuche,Delphine Tourbiez,Sophie Mangenot,Alexandra Calteau,Alexandra Calteau,Didier Mazel,Didier Mazel,Jean-Louis Nicolas,Annick Jacq,Frédérique Le Roux,Frédérique Le Roux,Frédérique Le Roux +22 more
TL;DR: It is shown that virulent strains cluster into two V. aestuarianus lineages independently of the sampling dates, suggesting that within these phylogenetically coherent virulent lineages a few strains have lost their pathogenicity.
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Non-fermentative gram-negative bacteria in hospital tap water and water used for haemodialysis and bronchoscope flushing: prevalence and distribution of antibiotic resistant strains.
Sara Vincenti,Gianluigi Quaranta,Concetta De Meo,Stefania Bruno,Maria Giovanna Ficarra,Serena Carovillano,Walter Ricciardi,Patrizia Laurenti +7 more
TL;DR: The findings highlight that the emergent antibiotic resistant opportunistic pathogens, such as R. pickettii and S. maltophilia, isolated from hospital environments could be potentially more dangerous than other more known waterborne pathogens, if not subjected to surveillance to direct the decontamination procedures.
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Acclimation conditions modify physiological response of the diatom Thalassiosira pseudonana to elevated CO2 concentrations in a nitrate-limited chemostat
TL;DR: Results suggest that T. pseudonana shifts carbon and energy fluxes in response to high CO2 and that acclimation time has a strong effect on the physiological response.
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Manganese limitation as a mechanism for reduced decomposition in soils under atmospheric nitrogen deposition
TL;DR: In this paper, the effect of manganese availability on decomposition dynamics in chronically N-enriched soils was evaluated by measuring litter mass loss, lignin relative abundance, and enzyme activity.
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