Nucleic acid-based approaches to investigate microbial-related cheese quality defects
Daniel J. O'Sullivan,Linda Giblin,Paul L.H. McSweeney,Jeremiah J. Sheehan,Paul D. Cotter,Paul D. Cotter +5 more
TLDR
The DNA-based methods that are available to detect/quantify spoilage bacteria, and relevant metabolic pathways in cheeses are reviewed and it is highlighted how these strategies can be employed to improve cheese quality and reduce the associated economic burden on cheese processors.Abstract:
The microbial profile of cheese is a primary determinant of cheese quality. Microorganisms can contribute to aroma and taste defects, form biogenic amines, cause gas and secondary fermentation defects, and can contribute to cheese pinking and mineral deposition issues. These defects may be as a result of seasonality and the variability in the composition of the milk supplied, variations in cheese processing parameters, as well as the nature and number of the non-starter microorganisms which come from the milk or other environmental sources. Such defects can be responsible for production and product recall costs and thus represent a significant economic burden for the dairy industry worldwide. Traditional non-molecular approaches are often considered biased and have inherently slow turnaround times. Molecular techniques can provide early and rapid detection of defects that result from the presence of specific spoilage microbes and, ultimately, assist in enhancing cheese quality and reducing costs. Here we review the DNA-based methods that are available to detect/quantify spoilage bacteria, and relevant metabolic pathways in cheeses and, in the process, highlight how these strategies can be employed to improve cheese quality and reduce the associated economic burden on cheese processors.read more
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Antibiotic resistance of Lactobacillus pentosus and Leuconostoc pseudomesenteroides isolated from naturally-fermented Aloreña table olives throughout fermentation process.
María del Carmen Casado Muñoz,Nabil Benomar,Leyre Lavilla Lerma,Antonio Gálvez,Hikmate Abriouel +4 more
TL;DR: Principal component analysis determined that the prevalence of antibiotic resistance in LAB throughout the fermentation process was highly dependent on the fermenter where the fermentation took place, and all Lb.
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Nitrogen cycling in the secondary nitrite maximum of the eastern tropical North Pacific off Costa Rica
Carolyn Buchwald,Alyson E. Santoro,Rachel H. R. Stanley,Rachel H. R. Stanley,Karen L. Casciotti +4 more
TL;DR: In this article, the authors present an analysis of rates of microbial nitrogen transformations in the oxygen deficient zone (ODZ) within the eastern tropical north Pacific ocean (ETNP) using a novel one-dimensional model using the distribution of nitrite and nitrate concentrations, along with their natural abundance nitrogen (N) and oxygen (O) isotope profiles.
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Antimicrobial and cytotoxic secondary metabolites from tropical leaf endophytes: Isolation of antibacterial agent pyrrocidine C from Lewia infectoria SNB-GTC2402.
Thiago M. Casella,Thiago M. Casella,Thiago M. Casella,Véronique Eparvier,Hugues Mandavid,Audrey Bendelac,Guillaume Odonne,Laura Dayan,Christophe Duplais,Laila S. Espindola,Didier Stien +10 more
TL;DR: A previously unreported compound named pyrrocidine C was isolated from Lewia infectoria SNB-GTC2402 and identified by spectroscopic analysis and identified as a cis-substituted decahydrofluorene with a quaternary carbon at C-5 and opposite stereochemistry atC-8 corresponding to C-6 of p Pyrrocidines A and B.
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Metagenomic insights into the human gut resistome and the forces that shape it
TL;DR: It is shown how metagenomic analysis of the human gut antibiotic resistome, compared across large populations and against environmental or agricultural resistomes, suggests a strong anthropogenic cause behind increasing antibiotic resistance in bacteria.
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Anticancer properties of a defensin like class IId bacteriocin Laterosporulin10
TL;DR: Evidence is provided that LS10 is an anticancer bacteriocin, which causes apoptotic and necrotic death of cancer cells at lower and higher concentrations, respectively.
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