Nucleic acid-based approaches to investigate microbial-related cheese quality defects
Daniel J. O'Sullivan,Linda Giblin,Paul L.H. McSweeney,Jeremiah J. Sheehan,Paul D. Cotter,Paul D. Cotter +5 more
TLDR
The DNA-based methods that are available to detect/quantify spoilage bacteria, and relevant metabolic pathways in cheeses are reviewed and it is highlighted how these strategies can be employed to improve cheese quality and reduce the associated economic burden on cheese processors.Abstract:
The microbial profile of cheese is a primary determinant of cheese quality. Microorganisms can contribute to aroma and taste defects, form biogenic amines, cause gas and secondary fermentation defects, and can contribute to cheese pinking and mineral deposition issues. These defects may be as a result of seasonality and the variability in the composition of the milk supplied, variations in cheese processing parameters, as well as the nature and number of the non-starter microorganisms which come from the milk or other environmental sources. Such defects can be responsible for production and product recall costs and thus represent a significant economic burden for the dairy industry worldwide. Traditional non-molecular approaches are often considered biased and have inherently slow turnaround times. Molecular techniques can provide early and rapid detection of defects that result from the presence of specific spoilage microbes and, ultimately, assist in enhancing cheese quality and reducing costs. Here we review the DNA-based methods that are available to detect/quantify spoilage bacteria, and relevant metabolic pathways in cheeses and, in the process, highlight how these strategies can be employed to improve cheese quality and reduce the associated economic burden on cheese processors.read more
Citations
More filters
Journal ArticleDOI
The Epidemiology of Carbapenem-Resistant Enterobacteriaceae: The Impact and Evolution of a Global Menace.
TL;DR: The evolution of CRE is discussed, with a focus on the epidemiology of the CPE pandemic; risk factors for colonization and infection with the most common transmissible CPE worldwide, Klebsiella pneumoniae carbapenemase-producing K. pneumoniae are reviewed; and strategies used to halt the striking spread of these deadly pathogens are presented.
Journal ArticleDOI
The interplay of microbially mediated and abiotic reactions in the biogeochemical Fe cycle
TL;DR: The major microbially mediated and abiotic reactions in the biogeochemical Fe cycle are discussed and an integrated overview of biotic and chemically mediated redox transformations is provided.
Journal ArticleDOI
Contamination of water resources by pathogenic bacteria
TL;DR: This review paper attempts to develop the first comprehensive single source of existing information on pathogen contamination in multiple types of water resources, and describes the challenges associated with using indicator organisms.
Journal ArticleDOI
Traditional cheeses: Rich and diverse microbiota with associated benefits
Marie-Christine Montel,Solange Buchin,Adrien Mallet,Céline Delbès-Paus,Dominique A. Vuitton,Nathalie Desmasures,Françoise Berthier +6 more
TL;DR: In the future, the use of meta-omics methods should help to decipher how traditional cheese ecosystems form and function, opening the way to new methods of risk-benefit management from farm to ripened cheese.
Journal ArticleDOI
Microbial functional genes involved in nitrogen fixation, nitrification and denitrification in forest ecosystems
TL;DR: In this article, the abundance and community structure of functional genes involved in the biogeochemical cycling of N in forest soils offers an approach to directly link microbial groups to soil characteristics and ecosystem processes.
References
More filters
Journal ArticleDOI
Recent advances in molecular techniques to study microbial communities in food-associated matrices and processes.
TL;DR: Molecular methods will allow us to surpass the authors' current culturing limitations, thus revealing the extent and importance of the 'non-culturable' microbial flora that occurs in food matrices and production.
Journal ArticleDOI
Challenges in applying microarrays to environmental studies.
TL;DR: Adapting microarray hybridization for use in environmental studies faces several challenges associated with specificity, sensitivity and quantitation.
Journal ArticleDOI
Monitoring bacterial communities in raw milk and cheese by culture-dependent and -independent 16S rRNA gene-based analyses.
TL;DR: Direct and culture-dependent approaches produced complementary information, thus generating a more accurate view of milk and cheese microbial ecology.
Journal ArticleDOI
The potential of a polyphasic PCR-dGGE approach in evaluating microbial diversity of natural whey cultures for water-buffalo Mozzarella cheese production: bias of culture-dependent and culture-independent analyses.
TL;DR: The developed PCR-DGGE analysis of the "cultivable" community showed good potential in evaluating microbial diversity of a dairy environment and usefully highlighted the bias introduced by selective amplification when compared to the analysis ofThe total community from NWC and allowed suitability of media and growth conditions to be evaluated.
Journal ArticleDOI
Detection, growth, and amine-producing capacity of lactobacilli in cheese.
Han Joosten,M. D. Northolt +1 more
TL;DR: Cheesemaking experiments using a low inoculum concentration of the histidinedecarboxylating L. buchneri strain St2A showed that, under conditions of accelerated proteolysis, histamine may accumulate rapidly; after 3 months of ripening, 410 mg/kg was found.
Related Papers (5)
QIIME allows analysis of high-throughput community sequencing data.
J. Gregory Caporaso,Justin Kuczynski,Jesse Stombaugh,Kyle Bittinger,Frederic D. Bushman,Elizabeth K. Costello,Noah Fierer,Antonio Gonzalez Peña,Julia K. Goodrich,Jeffrey I. Gordon,Gavin A. Huttley,Scott T. Kelley,Dan Knights,Jeremy E. Koenig,Ruth E. Ley,Catherine A. Lozupone,Daniel McDonald,Brian D. Muegge,Meg Pirrung,Jens Reeder,Joel Sevinsky,Peter J. Turnbaugh,William A. Walters,Jeremy Widmann,Tanya Yatsunenko,Jesse R. Zaneveld,Rob Knight,Rob Knight +27 more
Introducing mothur: Open-Source, Platform-Independent, Community-Supported Software for Describing and Comparing Microbial Communities
Patrick D. Schloss,Patrick D. Schloss,Sarah L. Westcott,Sarah L. Westcott,Thomas Ryabin,Justine R. Hall,Martin Hartmann,Emily B. Hollister,Ryan A. Lesniewski,Brian B. Oakley,Donovan H. Parks,Courtney J. Robinson,Jason W. Sahl,Blaz Stres,Gerhard G. Thallinger,David J. Van Horn,Carolyn F. Weber +16 more