Journal ArticleDOI
Overcoming the Incompatibility Challenge in Chemoenzymatic and Multi-Catalytic Cascade Reactions.
TLDR
This Minireview will summarize recent synthetic examples of (chemo)enzymatic cascade reactions and outline promising methods for their spatial control either by using bio-derived or synthetic systems.Abstract:
Multi-catalytic cascade reactions bear a great potential to minimize downstream and purification steps, leading to a drastic reduction of the produced waste. In many examples, the compatibility of chemo- and biocatalytic steps could be easily achieved. Problems associated with the incompatibility of the catalysts and their reactions, however, are very frequent. Cascade-like reactions can hardly occur in this way. One possible solution to combine, in principle, incompatible chemo- and biocatalytic reactions is the defined control of the microenvironment by compartmentalization or scaffolding. Current methods for the control of the microenvironment of biocatalysts go far beyond classical enzyme immobilization and are thus believed to be very promising tools to overcome incompatibility issues and to facilitate the synthetic application of cascade reactions. In this Minireview, we will summarize recent synthetic examples of (chemo)enzymatic cascade reactions and outline promising methods for their spatial control either by using bio-derived or synthetic systems.read more
Citations
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Journal ArticleDOI
Opportunities and challenges for combining chemo- and biocatalysis
Florian Rudroff,Marko D. Mihovilovic,Harald Gröger,Radka Snajdrova,Hans Iding,Uwe T. Bornscheuer +5 more
TL;DR: This Review focuses on efforts to combine chemo- and biocatalysts, outlining the opportunities achievable by this approach and also efforts to overcome any incompatibilities between these different systems.
Journal ArticleDOI
Biocatalytic Oxidation Reactions: A Chemist's Perspective
JiaJia Dong,Elena Fernández-Fueyo,Frank Hollmann,Caroline E. Paul,Milja Pesic,Sandy Schmidt,Yonghua Wang,Sabry H. H. Younes,Wuyuan Zhang +8 more
TL;DR: The most important recent developments in the field of biocatalytic oxidation chemistry are critically summarised and the most pressing bottlenecks as well as promising solutions are identified.
Journal ArticleDOI
Photo-Biocatalysis: Biotransformations in the Presence of Light
Luca Schmermund,Valentina Jurkaš,Fatma Feyza Özgen,Giovanni Davide Barone,Hanna C. Büchsenschütz,Christoph K. Winkler,Sandy Schmidt,Robert Kourist,Wolfgang Kroutil +8 more
TL;DR: An overview of the various approaches for using light in biocatalysis is given and enzymes with a light switchable moiety have been investigated to turn off/on or tune the actual reaction.
Journal ArticleDOI
On the taught new tricks of enzymes immobilization: An all-inclusive overview
TL;DR: This review describes the choices of support materials and cross-linkers together with several mechanisms that influence the performance, stabilization and hyperactivation of immobilized enzymes.
References
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Journal ArticleDOI
Combination of enzymes and metal catalysts. A powerful approach in asymmetric catalysis.
Oscar Pàmies,Jan-E. Bäckvall +1 more
Journal ArticleDOI
Cascade Reactions in Multicompartmentalized Polymersomes
Ruud J. R. W. Peters,Maïté Marguet,Maïté Marguet,Sébastien Marais,Marco W. Fraaije,Jan C. M. van Hest,Sébastien Lecommandoux,Sébastien Lecommandoux +7 more
TL;DR: In this paper, an original cofactor-dependent three-enzyme cascade reaction is performed, using either compatible or incompatible enzymes, which takes place across multiple compartments, which shows structural resemblance to the cell and its organelles.
Journal ArticleDOI
Artificial Biocatalytic Linear Cascades for Preparation of Organic Molecules
TL;DR: The review introduces a systematic classification of the cascades according to the number of enzymes in the linear sequence and differentiates between cascades involving exclusively enzymes and combinations of enzymes with non-natural catalysts or chemical steps.
Journal ArticleDOI
Positional assembly of enzymes in polymersome nanoreactors for cascade reactions.
Dennis M. Vriezema,Paula M. L. Garcia,Paula M. L. Garcia,Nfflria Sancho Oltra,Nfflria Sancho Oltra,Nikos S. Hatzakis,Suzanne M. Kuiper,Roeland J. M. Nolte,Alan E. Rowan,Jan C. M. van Hest +9 more
TL;DR: In a good position: Nanoreactors can be constructed by the controlled positioning of glucose oxidase (GOX) and horseradish peroxidase (HRP) within the central water pool and block-copolymer membrane of polymersomes.
Journal ArticleDOI
A virus-based single-enzyme nanoreactor
Marta Comellas-Aragonès,Hans Engelkamp,Victor I. Claessen,Nico A. J. M. Sommerdijk,Alan E. Rowan,Peter C. M. Christianen,Jan C. Maan,Benedictus J. M. Verduin,Jeroen J. L. M. Cornelissen,Roeland J. M. Nolte +9 more
TL;DR: The incorporation of individual horseradish peroxidase enzymes in the inner cavity of a virus is reported, and single-molecule studies on their enzymatic behaviour are described, showing that the virus capsid is permeable for substrate and product and that this permeability can be altered by changing pH.