PatentDOI
Production of high titer helper-free retroviruses by transient transfection
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TLDR
In this article, a method for producing high-titer, helper-free infectious retroviruses is disclosed which employs a novel strategy that uses transient transfection of new retroviral producer cell lines, ecotropic line BOSC 23 and amphotropic line CAK 8.Abstract:
A method for producing high-titer, helper-free infectious retroviruses is disclosed which employs a novel strategy that uses transient transfection of new retroviral producer cell lines, ecotropic line BOSC 23 and amphotropic line CAK 8, both of which cell lines and their precursor cell lines are disclosed. Because of the advantages over stable packaging cell lines, the BOSC 23 and CAK 8 transient transfection systems greatly facilitate and extend the use of helper-free retroviral vectors. The cell lines and corresponding methods possess wide application in both the medical and biotechnical fields, including gene therapy. These potential applications are disclosed and illustrated.read more
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Neoplastic transformation by Notch requires nuclear localization.
TL;DR: Direct evidence that N1ic must accumulate in the nucleus to induce transformation of RKE cells is reported and the minimal domain of N1is defined, which is likely to be through a CBF1-independent pathway.
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Method for obtaining retroviral packaging cell lines producing high transducing efficiency retroviral supernatant
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Bruton’s tyrosine kinase regulates apoptosis and JNK/SAPK kinase activity
Yuko Kawakami,Toru Miura,Reid P. Bissonnette,Daisuke Hata,Wasif N. Khan,Toshio Kitamura,Mari Maeda-Yamamoto,Stephen E. Hartman,Libo Yao,Frederick W. Alt,Toshiaki Kawakami +10 more
TL;DR: The notion that Btk regulates apoptosis through the JNK activation is supported, as mast cells derived from Bruton's tyrosine kinase-defective xid or btk null mice showed greater expansion in culture containing interleukin-3 than those from wild-type (wt) mice.
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The presence of host-derived HLA-DR1 on human immunodeficiency virus type 1 increases viral infectivity.
TL;DR: It is suggested that HLA-DR-1-bearing HIV-1 particles had a greater infectivity per picogram of viral p24 protein than H LA-DR1-free virions and that the presence of virion-bound cellular HLA -DR1 is associated with faster kinetics of virus infection.
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TLS/FUS, a pro‐oncogene involved in multiple chromosomal translocations, is a novel regulator of BCR/ABL‐mediated leukemogenesis
Danilo Perrotti,Silvia Bonatti,Rossana Trotta,Robert Martinez,Tomasz Skorski,Paolo Salomoni,Emanuela Grassilli,Renato V. Iozzo,Denise R. Cooper,Bruno Calabretta +9 more
TL;DR: Results suggest that FUS might function as a regulator of BCR/ABL leukemogenesis, promoting growth factor independence and preventing differentiation via modulation of cytokine receptor expression.
References
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Characteristics of a Human Cell Line Transformed by DNA from Human Adenovirus Type 5
TL;DR: Human embryonic kidney cells have been transformed by exposing cells to sheared fragments of adenovirus type 5 DNA, and the transformed cells exhibited many of the characteristics of transformation including the elaboration of a virus-specific tumour antigen.
Journal ArticleDOI
Vaccination with Irradiated Tumor Cells Engineered to Secrete Murine Granulocyte-Macrophage Colony-Stimulating Factor Stimulates Potent, Specific, and Long-Lasting Anti-Tumor Immunity
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Induction of chronic myelogenous leukemia in mice by the P210bcr/abl gene of the Philadelphia chromosome
TL;DR: It is demonstrated that P210bcr/abl expression can induce chronic myelogenous leukemia and retrovirus-mediated expression of the protein provides a murine model system for further analysis of the disease.
Journal ArticleDOI
The Basic Science of Gene Therapy
TL;DR: A large number of key technical issues need to be resolved before gene therapy can be safely and effectively applied in the clinic, and future technological developments will be critical for the successful practice of gene therapy.
Journal Article
Improved Retroviral Vectors for Gene Transfer and Expression
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TL;DR: A set of murine retrovirus-based vectors that include unique cloning sites for insertion of cDNAs such that the cDNA can be driven by either the retroviral long terminal repeat, the immediate early promoter of human cytomegalovirus, or the simian virus 40 early promoter are described.
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