PatentDOI
Production of high titer helper-free retroviruses by transient transfection
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TLDR
In this article, a method for producing high-titer, helper-free infectious retroviruses is disclosed which employs a novel strategy that uses transient transfection of new retroviral producer cell lines, ecotropic line BOSC 23 and amphotropic line CAK 8.Abstract:
A method for producing high-titer, helper-free infectious retroviruses is disclosed which employs a novel strategy that uses transient transfection of new retroviral producer cell lines, ecotropic line BOSC 23 and amphotropic line CAK 8, both of which cell lines and their precursor cell lines are disclosed. Because of the advantages over stable packaging cell lines, the BOSC 23 and CAK 8 transient transfection systems greatly facilitate and extend the use of helper-free retroviral vectors. The cell lines and corresponding methods possess wide application in both the medical and biotechnical fields, including gene therapy. These potential applications are disclosed and illustrated.read more
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RhoB alteration is necessary for apoptotic and antineoplastic responses to farnesyltransferase inhibitors.
Ai-xue Liu,Wei Du,Wei Du,Jeh-Ping Liu,Thomas M. Jessell,George C. Prendergast,George C. Prendergast +6 more
TL;DR: Genetic proof is offered of the hypothesis that RhoB-GG is a crucial mediator of the antineoplastic effects of FTIs and the apoptotic defect of −/− cells compromised the antitumor efficacy of FTI in xenograft assays.
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Modulation of the F-actin cytoskeleton by c-Abl tyrosine kinase in cell spreading and neurite extension
Pamela J. Woodring,E. David Litwack,Dennis D.M. O'Leary,Ginger R. Lucero,Jean Y. J. Wang,Tony Hunter +5 more
TL;DR: It is shown that c-Abl stimulated the formation of actin microspikes in fibroblasts spreading on fibronectin, and the reciprocal regulation between F-actin and the c- Abl tyrosine kinase may provide a self-limiting mechanism in the control of act in cytoskeleton dynamics.
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Mammalian NUMB is an evolutionarily conserved signaling adapter protein that specifies cell fate.
Joseph M. Verdi,Rosemarie Schmandt,Arash Bashirullah,Sara Jacob,Ralph Salvino,Constance G. Craig,Amgen Est Program,Howard D. Lipshitz,C. Jane McGlade +8 more
TL;DR: The results demonstrate that mNUMB is an evolutionarily conserved functional homologue of d NUMB, and establish a link to tyrosine-kinase-mediated signal transduction pathways, and suggest that m NUMB and dNUMB are new members of a family of signaling adapter molecules that mediate conserved cell-fate decisions during development.
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Modulation of HIV-1 infectivity by MAPK, a virion-associated kinase.
Jean Marc Jacque,Angela M Mann,Hervé Enslen,Natalia Sharova,Beda Brichacek,Roger J. Davis,Mario Stevenson +6 more
TL;DR: Regulation of an early step in HIV‐1 infection by the host cell MAPK signal transduction pathway by the mitogen‐activated protein kinase (ERK/MAPK) is demonstrated.
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Resistance to experimental tumorigenesis in cells of a long-lived mammal, the naked mole-rat (Heterocephalus glaber)
TL;DR: Rapid crisis is a response of oncogene‐expressing NMR cells to growth in an in vivo environment, which requires anchorage independence, and hTERT permits cells to avoid crisis and to achieve malignant tumor growth.
References
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The Basic Science of Gene Therapy
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