PatentDOI
Production of high titer helper-free retroviruses by transient transfection
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TLDR
In this article, a method for producing high-titer, helper-free infectious retroviruses is disclosed which employs a novel strategy that uses transient transfection of new retroviral producer cell lines, ecotropic line BOSC 23 and amphotropic line CAK 8.Abstract:Â
A method for producing high-titer, helper-free infectious retroviruses is disclosed which employs a novel strategy that uses transient transfection of new retroviral producer cell lines, ecotropic line BOSC 23 and amphotropic line CAK 8, both of which cell lines and their precursor cell lines are disclosed. Because of the advantages over stable packaging cell lines, the BOSC 23 and CAK 8 transient transfection systems greatly facilitate and extend the use of helper-free retroviral vectors. The cell lines and corresponding methods possess wide application in both the medical and biotechnical fields, including gene therapy. These potential applications are disclosed and illustrated.read more
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The amino-terminal domain of CCR2 is both necessary and sufficient for high affinity binding of monocyte chemoattractant protein 1. Receptor activation by a pseudo-tethered ligand.
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STAT5 signaling is required for the efficient induction and maintenance of CML in mice.
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A carboxy-terminal domain of ELL is required and sufficient for immortalization of myeloid progenitors by MLL-ELL.
Jorge F. DiMartino,Trissa Miller,Paul M. Ayton,Theresa Landewe,Jay L. Hess,Jay L. Hess,Jay L. Hess,Michael L. Cleary,Ali Shilatifard,Ali Shilatifard,Ali Shilatifard +10 more
TL;DR: Data indicate that neoplastic transformation by the MLL-ELL fusion protein is likely to result from aberrant transcriptional activation of MLL target genes, and suggest that conversion of M LL to a constitutive transcriptional activator may be a general model for its oncogenic conversion in myeloid leukemias.
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Compositions and methods for determining anti-viral drug susceptibility and resistance and anti-viral drug screening
TL;DR: In this article, a method for determining susceptibility for an anti-viral drug comprising of introducing a resistance test vector comprising a patient-derived segment and an indicator gene into a host cell is presented.
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Early growth response gene 1 stimulates development of hematopoietic progenitor cells along the macrophage lineage at the expense of the granulocyte and erythroid lineages
TL;DR: The remarkable ability of EGr-1 to dictate macrophage development at the expense of development along other lineages resulted in failure of Egr-1-infected hematopoietic progenitors to repopulate the bone marrow and spleen, and thereby prevent death, in lethally irradiated mice.
References
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Characteristics of a Human Cell Line Transformed by DNA from Human Adenovirus Type 5
TL;DR: Human embryonic kidney cells have been transformed by exposing cells to sheared fragments of adenovirus type 5 DNA, and the transformed cells exhibited many of the characteristics of transformation including the elaboration of a virus-specific tumour antigen.
Journal ArticleDOI
Vaccination with Irradiated Tumor Cells Engineered to Secrete Murine Granulocyte-Macrophage Colony-Stimulating Factor Stimulates Potent, Specific, and Long-Lasting Anti-Tumor Immunity
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TL;DR: The results have important implications for the clinical use of genetically modified tumor cells as therapeutic cancer vaccines and the levels of anti-tumor immunity reported previously in cytokine gene transfer studies involving live, transduced cells could be achieved through the use of irradiated cells alone.
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Induction of chronic myelogenous leukemia in mice by the P210bcr/abl gene of the Philadelphia chromosome
TL;DR: It is demonstrated that P210bcr/abl expression can induce chronic myelogenous leukemia and retrovirus-mediated expression of the protein provides a murine model system for further analysis of the disease.
Journal ArticleDOI
The Basic Science of Gene Therapy
TL;DR: A large number of key technical issues need to be resolved before gene therapy can be safely and effectively applied in the clinic, and future technological developments will be critical for the successful practice of gene therapy.
Journal Article
Improved Retroviral Vectors for Gene Transfer and Expression
A D Miller,G J Rosman +1 more
TL;DR: A set of murine retrovirus-based vectors that include unique cloning sites for insertion of cDNAs such that the cDNA can be driven by either the retroviral long terminal repeat, the immediate early promoter of human cytomegalovirus, or the simian virus 40 early promoter are described.
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