PatentDOI
Production of high titer helper-free retroviruses by transient transfection
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TLDR
In this article, a method for producing high-titer, helper-free infectious retroviruses is disclosed which employs a novel strategy that uses transient transfection of new retroviral producer cell lines, ecotropic line BOSC 23 and amphotropic line CAK 8.Abstract:
A method for producing high-titer, helper-free infectious retroviruses is disclosed which employs a novel strategy that uses transient transfection of new retroviral producer cell lines, ecotropic line BOSC 23 and amphotropic line CAK 8, both of which cell lines and their precursor cell lines are disclosed. Because of the advantages over stable packaging cell lines, the BOSC 23 and CAK 8 transient transfection systems greatly facilitate and extend the use of helper-free retroviral vectors. The cell lines and corresponding methods possess wide application in both the medical and biotechnical fields, including gene therapy. These potential applications are disclosed and illustrated.read more
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STEAP: a prostate-specific cell-surface antigen highly expressed in human prostate tumors.
Rene S. Hubert,Igor Vivanco,Emily I. Chen,Shiva Rastegar,Kahan Leong,Steve Chappell Mitchell,Rashida Madraswala,Yanhong Zhou,James Kuo,Arthur B. Raitano,Aya Jakobovits,Douglas C. Saffran,Daniel E. H. Afar +12 more
TL;DR: The results support STEAP as a cell-surface tumor-antigen target for prostate cancer therapy and diagnostic imaging and support its role as a channel or transporter protein.
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References
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Vaccination with Irradiated Tumor Cells Engineered to Secrete Murine Granulocyte-Macrophage Colony-Stimulating Factor Stimulates Potent, Specific, and Long-Lasting Anti-Tumor Immunity
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Induction of chronic myelogenous leukemia in mice by the P210bcr/abl gene of the Philadelphia chromosome
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The Basic Science of Gene Therapy
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