PatentDOI
Production of high titer helper-free retroviruses by transient transfection
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TLDR
In this article, a method for producing high-titer, helper-free infectious retroviruses is disclosed which employs a novel strategy that uses transient transfection of new retroviral producer cell lines, ecotropic line BOSC 23 and amphotropic line CAK 8.Abstract:
A method for producing high-titer, helper-free infectious retroviruses is disclosed which employs a novel strategy that uses transient transfection of new retroviral producer cell lines, ecotropic line BOSC 23 and amphotropic line CAK 8, both of which cell lines and their precursor cell lines are disclosed. Because of the advantages over stable packaging cell lines, the BOSC 23 and CAK 8 transient transfection systems greatly facilitate and extend the use of helper-free retroviral vectors. The cell lines and corresponding methods possess wide application in both the medical and biotechnical fields, including gene therapy. These potential applications are disclosed and illustrated.read more
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Combined chemokine and cytokine gene transfer enhances antitumor immunity.
Dagmar Dilloo,Kevin B. Bacon,William Holden,Wanyun Zhong,Stefan Burdach,Albert Zlotnik,Malcolm K. Brenner +6 more
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Latest developments in gene transfer technology: achievements, perspectives, and controversies over therapeutic applications.
TL;DR: It is not possible to predict to what extent gene therapeutic interventions will be effective in patients, and in what time frame, but there is growing concern over the matter of safety of gene delivery and controversy has arisen over the proposal to begin in utero gene therapy clinical trials for the treatment of inherited genetic disorders.
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Requirement for C3G-dependent Rap1 activation for cell adhesion and embryogenesis.
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TL;DR: C3G‐dependent activation of Rap1 is required for adhesion and spreading of embryonic fibroblasts and for the early embryogenesis of the mouse.
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Notch signaling is a potent inducer of growth arrest and apoptosis in a wide range of B-cell malignancies
Patrick A. Zweidler-McKay,Yiping He,Lanwei Xu,Carlos G. Rodriguez,Fredrick G. Karnell,Andrea C. Carpenter,Jon C. Aster,David Allman,Warren S. Pear +8 more
TL;DR: The results suggest that therapies capable of activating Notch/Hes1 signaling may have therapeutic potential in a wide range of human B-cell malignancies.
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CD4-Independent Infection of Astrocytes by Human Immunodeficiency Virus Type 1: Requirement for the Human Mannose Receptor
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TL;DR: Results demonstrate the direct involvement of hMR in HIV-1 infection of astrocytes and suggest that HIV- 1 interaction with hMR plays an important role in AIDS-1 neuropathogenesis.
References
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Vaccination with Irradiated Tumor Cells Engineered to Secrete Murine Granulocyte-Macrophage Colony-Stimulating Factor Stimulates Potent, Specific, and Long-Lasting Anti-Tumor Immunity
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Induction of chronic myelogenous leukemia in mice by the P210bcr/abl gene of the Philadelphia chromosome
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The Basic Science of Gene Therapy
TL;DR: A large number of key technical issues need to be resolved before gene therapy can be safely and effectively applied in the clinic, and future technological developments will be critical for the successful practice of gene therapy.
Journal Article
Improved Retroviral Vectors for Gene Transfer and Expression
A D Miller,G J Rosman +1 more
TL;DR: A set of murine retrovirus-based vectors that include unique cloning sites for insertion of cDNAs such that the cDNA can be driven by either the retroviral long terminal repeat, the immediate early promoter of human cytomegalovirus, or the simian virus 40 early promoter are described.
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