PatentDOI
Production of high titer helper-free retroviruses by transient transfection
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TLDR
In this article, a method for producing high-titer, helper-free infectious retroviruses is disclosed which employs a novel strategy that uses transient transfection of new retroviral producer cell lines, ecotropic line BOSC 23 and amphotropic line CAK 8.Abstract:
A method for producing high-titer, helper-free infectious retroviruses is disclosed which employs a novel strategy that uses transient transfection of new retroviral producer cell lines, ecotropic line BOSC 23 and amphotropic line CAK 8, both of which cell lines and their precursor cell lines are disclosed. Because of the advantages over stable packaging cell lines, the BOSC 23 and CAK 8 transient transfection systems greatly facilitate and extend the use of helper-free retroviral vectors. The cell lines and corresponding methods possess wide application in both the medical and biotechnical fields, including gene therapy. These potential applications are disclosed and illustrated.read more
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Anaphase-promoting complex/cyclosome-dependent proteolysis of human cyclin A starts at the beginning of mitosis and is not subject to the spindle assembly checkpoint.
TL;DR: Microinjection of antibodies against subunits of the anaphase-promoting complex/cyclosome (APC/C) or against human Cdc20 (fizzy) arrested cells at metaphase and stabilized both cyclins A and B1 suggest that the APC/ C is activated as cells enter mitosis and immediately targets cyclin A for degradation, whereas the spindle assembly checkpoint delays the degradation of cyclin B1 until the metaphase to anaphases transition.
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A role for the Wnt gene family in hematopoiesis: expansion of multilineage progenitor cells.
TL;DR: It is concluded that W NTs stimulate the survival/proliferation of hematopoietic progenitors, demonstrating that WNTs comprise a novel class ofhematopOietic cell regulators.
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Identification and Characterization of a Constitutively Active STAT5 Mutant That Promotes Cell Proliferation
Mayumi Onishi,Tetsuya Nosaka,Kazuhide Misawa,Alice L.-F. Mui,Daniel M. Gorman,Martin McMahon,Atsushi Miyajima,Toshio Kitamura +7 more
TL;DR: This work has identified a constitutively active STAT5 mutant which has two amino acid substitutions; one is located upstream of the putative DNA binding domain (H299R), and the other is located in the transactivation domain (S711F) and indicated that a molecular basis for the constitutive activation is the stability of the phosphorylated form of the mutant STAT5.
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Regulation of a Senescence Checkpoint Response by the E2F1 Transcription Factor and p14 ARF Tumor Suppressor
TL;DR: It is shown that E2F1, a multifunctional transcription factor that binds the retinoblastoma (pRb) tumor suppressor and that can either promote or suppress tumorigenesis, induces a senescent phenotype when overexpressed in normal human fibroblasts and identifies p14ARF as a potentially important mediator of the senescence phenotype.
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A novel mechanism of gene regulation and tumor suppression by the transcription factor FKHR
TL;DR: In this paper, the authors demonstrate that rather than activation of IRS regulated genes (Class I transcripts), transcriptional repression of D-type cyclins (in Class III) is required for FKHR mediated inhibition of cell cycle progression and transformation.
References
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Vaccination with Irradiated Tumor Cells Engineered to Secrete Murine Granulocyte-Macrophage Colony-Stimulating Factor Stimulates Potent, Specific, and Long-Lasting Anti-Tumor Immunity
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Induction of chronic myelogenous leukemia in mice by the P210bcr/abl gene of the Philadelphia chromosome
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The Basic Science of Gene Therapy
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Journal Article
Improved Retroviral Vectors for Gene Transfer and Expression
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TL;DR: A set of murine retrovirus-based vectors that include unique cloning sites for insertion of cDNAs such that the cDNA can be driven by either the retroviral long terminal repeat, the immediate early promoter of human cytomegalovirus, or the simian virus 40 early promoter are described.
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