PatentDOI
Production of high titer helper-free retroviruses by transient transfection
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TLDR
In this article, a method for producing high-titer, helper-free infectious retroviruses is disclosed which employs a novel strategy that uses transient transfection of new retroviral producer cell lines, ecotropic line BOSC 23 and amphotropic line CAK 8.Abstract:
A method for producing high-titer, helper-free infectious retroviruses is disclosed which employs a novel strategy that uses transient transfection of new retroviral producer cell lines, ecotropic line BOSC 23 and amphotropic line CAK 8, both of which cell lines and their precursor cell lines are disclosed. Because of the advantages over stable packaging cell lines, the BOSC 23 and CAK 8 transient transfection systems greatly facilitate and extend the use of helper-free retroviral vectors. The cell lines and corresponding methods possess wide application in both the medical and biotechnical fields, including gene therapy. These potential applications are disclosed and illustrated.read more
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Journal ArticleDOI
Vascular Gene Transfer of the Human Inducible Nitric Oxide Synthase: Characterization of Activity and Effects on Myointimal Hyperplasia
Edith Tzeng,Larry L. Shears,Paul D. Robbins,Bruce R. Pitt,David A. Geller,Simon C. Watkins,Richard L. Simmons,Timothy R. Billiar +7 more
TL;DR: In vitro findings suggest that vascular iNOS gene transfer may be feasible and a low gene transfer efficiency may be sufficient to inhibit myointimal hyperplasia following arterial balloon injury, although a source of BH4 may be required.
Journal ArticleDOI
Genetic deletion of receptor for hyaluronan-mediated motility (Rhamm) attenuates the formation of aggressive fibromatosis (desmoid tumor)
TL;DR: Cell culture studies show that Rhamm regulates cell proliferation in both fibroblasts and fibromatosis cells under conditions of low density, but not high density, and this work provides the first genetic evidence implicating RhamM in tumor pathology.
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Method of enhancing proliferation or differentiation of hematopoietic stem cells using Wnt polypeptides
TL;DR: In this paper, in vitro and in vivo methods for enhancing proliferation or differentiation of a hematopoietic stem/progenitor cell using a Wnt polypeptide, and optionally another cytokine, are described.
Journal ArticleDOI
Constitutive activation of STAT5 by a point mutation in the SH2 domain.
Kouichi Ariyoshi,Tetsuya Nosaka,Koji Yamada,Koji Yamada,Mayumi Onishi,Yoshitomo Oka,Atsushi Miyajima,Toshio Kitamura +7 more
TL;DR: Another constitutively active mutant is reported, STAT5A-N642H which has a single point mutation in its SH2 domain, identified using the same strategy as that used to identifySTAT5A1*6, and showed identical properties to those of STAT5B*6 both biochemically and biologically.
Journal ArticleDOI
CCAAT/Enhancer binding proteins repress the leukemic phenotype of acute myeloid leukemia
Bao Tran H. Truong,Bao Tran H. Truong,Young-Jin Lee,Young-Jin Lee,Tracey Lodie,Tracey Lodie,Dorothy J. Park,Dorothy J. Park,Danilo Perrotti,Danilo Perrotti,Naohide Watanabe,Naohide Watanabe,H. Phillip Koeffler,H. Phillip Koeffler,Hideaki Nakajima,Hideaki Nakajima,Daniel G. Tenen,Daniel G. Tenen,Scott C. Kogan,Scott C. Kogan,Scott C. Kogan +20 more
TL;DR: The results support the hypothesis that induction of C/EBP activity is a critical effect of tRA in APL and suggest that targeted modulation of C /EBP activities could provide a new approach to therapy of AML.
References
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Characteristics of a Human Cell Line Transformed by DNA from Human Adenovirus Type 5
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Vaccination with Irradiated Tumor Cells Engineered to Secrete Murine Granulocyte-Macrophage Colony-Stimulating Factor Stimulates Potent, Specific, and Long-Lasting Anti-Tumor Immunity
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Induction of chronic myelogenous leukemia in mice by the P210bcr/abl gene of the Philadelphia chromosome
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The Basic Science of Gene Therapy
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Journal Article
Improved Retroviral Vectors for Gene Transfer and Expression
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TL;DR: A set of murine retrovirus-based vectors that include unique cloning sites for insertion of cDNAs such that the cDNA can be driven by either the retroviral long terminal repeat, the immediate early promoter of human cytomegalovirus, or the simian virus 40 early promoter are described.
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