PatentDOI
Production of high titer helper-free retroviruses by transient transfection
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TLDR
In this article, a method for producing high-titer, helper-free infectious retroviruses is disclosed which employs a novel strategy that uses transient transfection of new retroviral producer cell lines, ecotropic line BOSC 23 and amphotropic line CAK 8.Abstract:
A method for producing high-titer, helper-free infectious retroviruses is disclosed which employs a novel strategy that uses transient transfection of new retroviral producer cell lines, ecotropic line BOSC 23 and amphotropic line CAK 8, both of which cell lines and their precursor cell lines are disclosed. Because of the advantages over stable packaging cell lines, the BOSC 23 and CAK 8 transient transfection systems greatly facilitate and extend the use of helper-free retroviral vectors. The cell lines and corresponding methods possess wide application in both the medical and biotechnical fields, including gene therapy. These potential applications are disclosed and illustrated.read more
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Use of dominant negative mutants of the hepadnaviral core protein as antiviral agents
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Monosomy of chromosome 10 associated with dysregulation of epidermal growth factor signaling in glioblastomas.
Ajay Yadav,Jaclyn J. Renfrow,Denise M. Scholtens,Hehuang Xie,George E. Duran,Claudia Bredel,Claudia Bredel,Hannes Vogel,James P. Chandler,Arnab Chakravarti,Pierre A. Robe,Sunit Das,Adrienne C. Scheck,John A. Kessler,Marcelo B. Soares,Branimir I. Sikic,Griffith R. Harsh,Markus Bredel,Markus Bredel,Markus Bredel +19 more
TL;DR: Haploinsufficiency of the tumor suppressor ANXA7 due to monosomy of chromosome 10 provides a clinically relevant mechanism to augment EGFR signaling and patient survival and doubles tumorigenic potential of glioblastoma cells.
References
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Vaccination with Irradiated Tumor Cells Engineered to Secrete Murine Granulocyte-Macrophage Colony-Stimulating Factor Stimulates Potent, Specific, and Long-Lasting Anti-Tumor Immunity
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Induction of chronic myelogenous leukemia in mice by the P210bcr/abl gene of the Philadelphia chromosome
TL;DR: It is demonstrated that P210bcr/abl expression can induce chronic myelogenous leukemia and retrovirus-mediated expression of the protein provides a murine model system for further analysis of the disease.
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The Basic Science of Gene Therapy
TL;DR: A large number of key technical issues need to be resolved before gene therapy can be safely and effectively applied in the clinic, and future technological developments will be critical for the successful practice of gene therapy.
Journal Article
Improved Retroviral Vectors for Gene Transfer and Expression
A D Miller,G J Rosman +1 more
TL;DR: A set of murine retrovirus-based vectors that include unique cloning sites for insertion of cDNAs such that the cDNA can be driven by either the retroviral long terminal repeat, the immediate early promoter of human cytomegalovirus, or the simian virus 40 early promoter are described.
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