PatentDOI
Production of high titer helper-free retroviruses by transient transfection
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TLDR
In this article, a method for producing high-titer, helper-free infectious retroviruses is disclosed which employs a novel strategy that uses transient transfection of new retroviral producer cell lines, ecotropic line BOSC 23 and amphotropic line CAK 8.Abstract:
A method for producing high-titer, helper-free infectious retroviruses is disclosed which employs a novel strategy that uses transient transfection of new retroviral producer cell lines, ecotropic line BOSC 23 and amphotropic line CAK 8, both of which cell lines and their precursor cell lines are disclosed. Because of the advantages over stable packaging cell lines, the BOSC 23 and CAK 8 transient transfection systems greatly facilitate and extend the use of helper-free retroviral vectors. The cell lines and corresponding methods possess wide application in both the medical and biotechnical fields, including gene therapy. These potential applications are disclosed and illustrated.read more
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Induction of TNF-sensitive cellular phenotype by c-Myc involves p53 and impaired NF-kappaB activation.
Juha Klefström,Elena Arighi,Trevor D. Littlewood,Marja Jäättelä,Eero Saksela,Gerard I. Evan,Kari Alitalo +6 more
TL;DR: The present findings show that the cytotoxic activity of TNF towards oncoprotein‐expressing cells involves p53 and an impaired signaling for survival in such cells.
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SKAP-55 regulates integrin adhesion and formation of T cell-APC conjugates.
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Dominant Negative Mutants Implicate STAT5 in Myeloid Cell Proliferation and Neutrophil Differentiation
R. L. Ilaria,R. L. Ilaria,R. L. Ilaria,Robert G. Hawley,Robert G. Hawley,Robert G. Hawley,Richard A. Van Etten,Richard A. Van Etten,Richard A. Van Etten +8 more
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Amino-terminal signal transducer and activator of transcription (stat) domains regulate nuclear translocation and stat deactivation
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References
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Vaccination with Irradiated Tumor Cells Engineered to Secrete Murine Granulocyte-Macrophage Colony-Stimulating Factor Stimulates Potent, Specific, and Long-Lasting Anti-Tumor Immunity
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Induction of chronic myelogenous leukemia in mice by the P210bcr/abl gene of the Philadelphia chromosome
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The Basic Science of Gene Therapy
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Journal Article
Improved Retroviral Vectors for Gene Transfer and Expression
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TL;DR: A set of murine retrovirus-based vectors that include unique cloning sites for insertion of cDNAs such that the cDNA can be driven by either the retroviral long terminal repeat, the immediate early promoter of human cytomegalovirus, or the simian virus 40 early promoter are described.
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