PatentDOI
Production of high titer helper-free retroviruses by transient transfection
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TLDR
In this article, a method for producing high-titer, helper-free infectious retroviruses is disclosed which employs a novel strategy that uses transient transfection of new retroviral producer cell lines, ecotropic line BOSC 23 and amphotropic line CAK 8.Abstract:
A method for producing high-titer, helper-free infectious retroviruses is disclosed which employs a novel strategy that uses transient transfection of new retroviral producer cell lines, ecotropic line BOSC 23 and amphotropic line CAK 8, both of which cell lines and their precursor cell lines are disclosed. Because of the advantages over stable packaging cell lines, the BOSC 23 and CAK 8 transient transfection systems greatly facilitate and extend the use of helper-free retroviral vectors. The cell lines and corresponding methods possess wide application in both the medical and biotechnical fields, including gene therapy. These potential applications are disclosed and illustrated.read more
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Journal ArticleDOI
Gene Transfer into Hepatocytes and Human Liver Tissue by Baculovirus Vectors
TL;DR: Baculovirus-mediated gene expression declines rapidly in the hepatocellular carcinoma cell line Huh7 and more slowly in primary cultures of mouse hepatocytes, suggesting the development of a strategy using bacULoviral vectors for liver-directed gene therapy.
Journal ArticleDOI
Dimerization of MLL fusion proteins immortalizes hematopoietic cells.
Mary Ellen Martin,Thomas A. Milne,Thomas A. Milne,Sebastien Bloyer,Karine Galoian,Weiping Shen,Denise Gibbs,Hugh W. Brock,Robert K. Slany,Jay L. Hess +9 more
TL;DR: The data support a model in which either MLL dimerization/exon duplication or fusion to a transcriptional activator results in Hox gene upregulation and ultimately transformation.
Patent
Method for production of high titer virus and high efficiency retroviral mediated transduction of mammalian cells
TL;DR: In this paper, the authors proposed a retroviral packaging system, in which plasmids and vector transcripts are produced from high expression plasmid after stable or transient transfection in mammalian cells, and can then transduce a mammalian target cell by cocultivation or supernatant infection.
Journal ArticleDOI
Localized suppression of RhoA activity by Tyr31/118-phosphorylated paxillin in cell adhesion and migration
Asako Tsubouchi,Asako Tsubouchi,Junko Sakakura,Ryohei Yagi,Yuichi Mazaki,Erik Schaefer,Hajime Yano,Hisataka Sabe,Hisataka Sabe +8 more
TL;DR: It is concluded that Tyr31/118-phosphorylated paxillin serves as a template for the localized suppression of RhoA activity and is necessary for efficient membrane spreading and ruffling in adhesion and migration of NMuMG cells.
Journal ArticleDOI
Molecular dissection of Pax6 function: the specific roles of the paired domain and homeodomain in brain development
Nicole Haubst,Joachim Berger,Venugopal Radjendirane,Jochen Graw,Jack Favor,Grady F. Saunders,Anastassia Stoykova,Magdalena Götz +7 more
TL;DR: A key role of the PD in brain development is demonstrated and splicing is implicate as a pivotal factor regulating the potent neurogenic role of Pax6.
References
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Characteristics of a Human Cell Line Transformed by DNA from Human Adenovirus Type 5
TL;DR: Human embryonic kidney cells have been transformed by exposing cells to sheared fragments of adenovirus type 5 DNA, and the transformed cells exhibited many of the characteristics of transformation including the elaboration of a virus-specific tumour antigen.
Journal ArticleDOI
Vaccination with Irradiated Tumor Cells Engineered to Secrete Murine Granulocyte-Macrophage Colony-Stimulating Factor Stimulates Potent, Specific, and Long-Lasting Anti-Tumor Immunity
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TL;DR: The results have important implications for the clinical use of genetically modified tumor cells as therapeutic cancer vaccines and the levels of anti-tumor immunity reported previously in cytokine gene transfer studies involving live, transduced cells could be achieved through the use of irradiated cells alone.
Journal ArticleDOI
Induction of chronic myelogenous leukemia in mice by the P210bcr/abl gene of the Philadelphia chromosome
TL;DR: It is demonstrated that P210bcr/abl expression can induce chronic myelogenous leukemia and retrovirus-mediated expression of the protein provides a murine model system for further analysis of the disease.
Journal ArticleDOI
The Basic Science of Gene Therapy
TL;DR: A large number of key technical issues need to be resolved before gene therapy can be safely and effectively applied in the clinic, and future technological developments will be critical for the successful practice of gene therapy.
Journal Article
Improved Retroviral Vectors for Gene Transfer and Expression
A D Miller,G J Rosman +1 more
TL;DR: A set of murine retrovirus-based vectors that include unique cloning sites for insertion of cDNAs such that the cDNA can be driven by either the retroviral long terminal repeat, the immediate early promoter of human cytomegalovirus, or the simian virus 40 early promoter are described.
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