PatentDOI
Production of high titer helper-free retroviruses by transient transfection
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TLDR
In this article, a method for producing high-titer, helper-free infectious retroviruses is disclosed which employs a novel strategy that uses transient transfection of new retroviral producer cell lines, ecotropic line BOSC 23 and amphotropic line CAK 8.Abstract:
A method for producing high-titer, helper-free infectious retroviruses is disclosed which employs a novel strategy that uses transient transfection of new retroviral producer cell lines, ecotropic line BOSC 23 and amphotropic line CAK 8, both of which cell lines and their precursor cell lines are disclosed. Because of the advantages over stable packaging cell lines, the BOSC 23 and CAK 8 transient transfection systems greatly facilitate and extend the use of helper-free retroviral vectors. The cell lines and corresponding methods possess wide application in both the medical and biotechnical fields, including gene therapy. These potential applications are disclosed and illustrated.read more
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Podocin localizes in the kidney to the slit diaphragm area.
Séverine Roselli,Olivier Gribouval,Nicolas Boute,Mireille Sich,T Attié,Marie-Claire Gubler,Corinne Antignac +6 more
TL;DR: In situ hybridization and electron microscopy demonstrate that podocin is facing the slit diaphragm with its two ends in the cytoplasm of the foot processes, in agreement with its predicted structure, suggesting thatpodocin could serve to anchor directly or indirectly components of the slitdiaphragms to the cytoskeleton.
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A low-molecular-weight inhibitor against the chemokine receptor CXCR4: a strong anti-HIV peptide T140.
Hirokazu Tamamura,Younong Xu,Toshio Hattori,Xiaoyan Zhang,Rieko Arakaki,Kenji Kanbara,Akane Omagari,Akira Otaka,Toshiro Ibuka,Naoki Yamamoto,Hideki Nakashima,Nobutaka Fujii +11 more
TL;DR: This work reports the finding of novel 14-residue CXCR4 inhibitors, T134 and T140, on the basis of the T22 structure, which showed the highest inhibitory activity against HIV-1 entry and the strongest inhibitory effect on the binding of an anti-CX CR4 monoclonal antibody to CxCR4 among all the CXcr4 inhibitors that have been reported up to now.
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Transcription coactivator TRAP220 is required for PPARγ2-stimulated adipogenesis
Kai Ge,Mohamed Guermah,Chao-Xing Yuan,Chao-Xing Yuan,Mitsuhiro Ito,Annika E. Wallberg,Bruce M. Spiegelman,Robert G. Roeder +7 more
TL;DR: Data indicate that TRAP220 acts, via the TRAP complex, as a PPARγ2-selective coactivator and, accordingly, that it is specific for one fibroblast differentiation pathway (adipogenesis) relative to another (myogenesis).
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PU.1 regulates both cytokine-dependent proliferation and differentiation of granulocyte/macrophage progenitors.
TL;DR: In this paper, the authors demonstrate that mutation of the PU.1 gene causes a severe reduction in myeloid (granulocyte/macrophage) progenitors.
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Inhibitors of cyclin-dependent kinases induce features of replicative senescence in early passage human diploid fibroblasts
TL;DR: It is demonstrated that, in primary fibroblasts, inhibition of G1-specific Cdk activity is sufficient to induce phenotypic changes that normally occur at the end of their finite lifespan.
References
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Vaccination with Irradiated Tumor Cells Engineered to Secrete Murine Granulocyte-Macrophage Colony-Stimulating Factor Stimulates Potent, Specific, and Long-Lasting Anti-Tumor Immunity
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The Basic Science of Gene Therapy
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