PatentDOI
Production of high titer helper-free retroviruses by transient transfection
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TLDR
In this article, a method for producing high-titer, helper-free infectious retroviruses is disclosed which employs a novel strategy that uses transient transfection of new retroviral producer cell lines, ecotropic line BOSC 23 and amphotropic line CAK 8.Abstract:
A method for producing high-titer, helper-free infectious retroviruses is disclosed which employs a novel strategy that uses transient transfection of new retroviral producer cell lines, ecotropic line BOSC 23 and amphotropic line CAK 8, both of which cell lines and their precursor cell lines are disclosed. Because of the advantages over stable packaging cell lines, the BOSC 23 and CAK 8 transient transfection systems greatly facilitate and extend the use of helper-free retroviral vectors. The cell lines and corresponding methods possess wide application in both the medical and biotechnical fields, including gene therapy. These potential applications are disclosed and illustrated.read more
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Associations of FGF‐3 and FGF‐10 with signaling networks regulating tooth morphogenesis
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ALK Expression Defines a Distinct Group of T/Null Lymphomas (“ALK Lymphomas”) with a Wide Morphological Spectrum
Brunangelo Falini,Barbara Bigerna,Marco Fizzotti,Karen Pulford,Stefano Pileri,Georges Delsol,Antonino Carbone,Marco Paulli,Umberto Magrini,Umberto Magrini,Fabio Menestrina,Roberte Giardini,Silvana Pilotti,A Mezzelani,Barbara Ugolini,Monia Billi,Alessandra Pucciarini,Roberta Pacini,Pier Giuseppe Pelicci,L. Flenghi +19 more
TL;DR: It is suggested that the neoplastic lesion (the NPM-ALK gene) must be present both in the large anaplastic and small tumor cells, and that ALK-positive lymphomas lie on a spectrum, their position being defined by the ratio of small to large neoplastics cells.
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Interaction of the Ski Oncoprotein with Smad3 Regulates TGF-β Signaling
TL;DR: It is demonstrated that Ski is a novel component of the TGF-β signaling pathway and shed light on the mechanism of action of the Ski oncoprotein.
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Disruption of the regulatory beta subunit of protein kinase CK2 in mice leads to a cell-autonomous defect and early embryonic lethality.
Thierry Buchou,Muriel Vernet,Olivier Blond,Hans H. Jensen,Hervé Pointu,Birgitte Brinkmann Olsen,Claude Cochet,Olaf-Georg Issinger,Brigitte Boldyreff +8 more
TL;DR: It is shown that in mammals, CK2β is essential for viability at the cellular level, possibly because it acquired new functions during evolution, in contrast to what occurs with yeast cells, which can survive without functional CK2 β.
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Efficient screening of retroviral cDNA expression libraries.
Toshio Kitamura,Mayumi Onishi,Shigemi Kinoshita,Akira Shibuya,Atsushi Miyajima,Garry P. Nolan +5 more
TL;DR: A simple packaging system for production of high-titer retrovirus stock from cDNA libraries to establish a cDNA expression cloning system that is efficient enough to clone low-abundance cDNAs by their expression or function.
References
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Characteristics of a Human Cell Line Transformed by DNA from Human Adenovirus Type 5
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Vaccination with Irradiated Tumor Cells Engineered to Secrete Murine Granulocyte-Macrophage Colony-Stimulating Factor Stimulates Potent, Specific, and Long-Lasting Anti-Tumor Immunity
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Induction of chronic myelogenous leukemia in mice by the P210bcr/abl gene of the Philadelphia chromosome
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The Basic Science of Gene Therapy
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Improved Retroviral Vectors for Gene Transfer and Expression
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