PatentDOI
Production of high titer helper-free retroviruses by transient transfection
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TLDR
In this article, a method for producing high-titer, helper-free infectious retroviruses is disclosed which employs a novel strategy that uses transient transfection of new retroviral producer cell lines, ecotropic line BOSC 23 and amphotropic line CAK 8.Abstract:
A method for producing high-titer, helper-free infectious retroviruses is disclosed which employs a novel strategy that uses transient transfection of new retroviral producer cell lines, ecotropic line BOSC 23 and amphotropic line CAK 8, both of which cell lines and their precursor cell lines are disclosed. Because of the advantages over stable packaging cell lines, the BOSC 23 and CAK 8 transient transfection systems greatly facilitate and extend the use of helper-free retroviral vectors. The cell lines and corresponding methods possess wide application in both the medical and biotechnical fields, including gene therapy. These potential applications are disclosed and illustrated.read more
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Journal ArticleDOI
A stable human-derived packaging cell line for production of high titer retrovirus/vesicular stomatitis virus G pseudotypes
TL;DR: A human 293-derived retroviral packaging cell line (293GPG) capable of producing high titers of recombinant Moloney murine leukemia virus particles that have incorporated the vesicular stomatitis virus G (VSV-G) protein is generated.
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Requirement of ATM-Dependent Phosphorylation of Brca1 in the DNA Damage Response to Double-Strand Breaks
TL;DR: In this article, it was shown that the checkpoint protein kinase ATM (mutated in ataxia telangiectasia) was required for phosphorylation of Brca1 in response to ionizing radiation.
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Notch1 expression in early lymphopoiesis influences B versus T lineage determination.
John C. Pui,David Allman,Lanwei Xu,Susan DeRocco,Fredrick G. Karnell,Sonia Bakkour,Julia Y Lee,Tom Kadesch,Richard R. Hardy,Jon C. Aster,Warren S. Pear +10 more
TL;DR: The results suggest that Notch1 provides a key regulatory signal in determining T lymphoid versus B lymphoid lineage decisions, possibly by influencing lineage commitment from a common lymphoid progenitor cell.
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RIP : a novel protein containing a death domain that interacts with Fas/APO-1 (CD95) in yeast and causes cell death
TL;DR: Transient overexpression of RIP causes transfected cells to undergo the morphological changes characteristic of apoptosis, and these properties indicate that RIP is a novel form of apoptotic-inducing protein.
Journal ArticleDOI
Control of cellular cholesterol efflux by the nuclear oxysterol receptor LXRα
Asha Venkateswaran,Bryan A. Laffitte,Sean B. Joseph,Puiying A. Mak,Damien C. Wilpitz,Peter A. Edwards,Peter Tontonoz +6 more
TL;DR: The results indicate that cellular cholesterol efflux is controlled, at least in part, at the level of transcription by a nuclear receptor-signaling pathway, and suggest a model in which activation of LXRs by oxysterols in response to cellular sterol loading leads to induction of the ABCA1 transporter and the stimulation of lipid efflux to extracellular acceptors.
References
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Vaccination with Irradiated Tumor Cells Engineered to Secrete Murine Granulocyte-Macrophage Colony-Stimulating Factor Stimulates Potent, Specific, and Long-Lasting Anti-Tumor Immunity
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The Basic Science of Gene Therapy
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Journal Article
Improved Retroviral Vectors for Gene Transfer and Expression
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