PatentDOI
Production of high titer helper-free retroviruses by transient transfection
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TLDR
In this article, a method for producing high-titer, helper-free infectious retroviruses is disclosed which employs a novel strategy that uses transient transfection of new retroviral producer cell lines, ecotropic line BOSC 23 and amphotropic line CAK 8.Abstract:
A method for producing high-titer, helper-free infectious retroviruses is disclosed which employs a novel strategy that uses transient transfection of new retroviral producer cell lines, ecotropic line BOSC 23 and amphotropic line CAK 8, both of which cell lines and their precursor cell lines are disclosed. Because of the advantages over stable packaging cell lines, the BOSC 23 and CAK 8 transient transfection systems greatly facilitate and extend the use of helper-free retroviral vectors. The cell lines and corresponding methods possess wide application in both the medical and biotechnical fields, including gene therapy. These potential applications are disclosed and illustrated.read more
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Journal ArticleDOI
Antibody-Mediated Targeting of Replication-Competent Retroviral Vectors
Chien Kuo Tai,Christopher R. Logg,Jinha M. Park,W. French Anderson,Michael F. Press,Noriyuki Kasahara,Noriyuki Kasahara +6 more
TL;DR: Interestingly, HER2-expressing human breast cancer cells did not show enhancement of transduction despite efficient antibody-mediated cell surface binding, suggesting that target cell-specific parameters markedly affect the efficiency of post-binding entry processes.
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Amplification and overexpression of PRUNE in human sarcomas and breast carcinomas - a possible mechanism for altering the nm23-H1 activity
Anne Forus,Anna D’Angelo,Jørn Henriksen,Giuseppe Merla,Gunhild Mari Mælandsmo,Vivi Ann Flørenes,Stefano Olivieri,Bodil Bjerkehagen,Leonardo A. Meza-Zepeda,Francesca Del Vecchio Blanco,Christoph R. Müller,Francesca Sanvito,Juha Kononen,Juha Kononen,Jahn M. Nesland,Øystein Fodstad,Alexandre Reymond,Olli Kallioniemi,Gianluigi Arrigoni,Andrea Ballabio,Ola Myklebost,Massimo Zollo +21 more
TL;DR: In this article, the authors found that amplification of PRUNE was associated with high mRNA and moderate to high protein levels in malignant sarcoma cells, and that 9/13 patients with PRUNE amplification developed metastases.
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Continuous Intravascular Secretion of Endostatin in Mice from Transduced Hematopoietic Stem Cells
Robert Pawliuk,Thomas Bachelot,Omar Zurkiya,Anna Eriksson,Yihai Cao,Philippe Leboulch,Philippe Leboulch +6 more
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Ovol2 Suppresses Cell Cycling and Terminal Differentiation of Keratinocytes by Directly Repressing c-Myc and Notch1
Julie Wells,Briana Lee,Anna Q. Cai,Adrine Karapetyan,Wan-Ju Lee,Elizabeth L. Rugg,Satrajit Sinha,Qing Nie,Xing Dai +8 more
TL;DR: It is shown that depletion of Ovol2 leads to transient cell expansion but a loss of cells with long term proliferation potential, and shed light on how an epidermal cell maintains a proliferation-competent and differentiation-resistant state.
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Spinophilin Stabilizes Cell Surface Expression of α2B-Adrenergic Receptors
TL;DR: Evidence that α2-AR interaction with spinophilin contributes to cell surface stabilization of the receptor is provided and two independent experimental approaches demonstrated that agonist-evoked internalization of HA-α2B-AR was accelerated in mouse embryo fibroblasts derived from Sp–/– mice.
References
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Journal ArticleDOI
Characteristics of a Human Cell Line Transformed by DNA from Human Adenovirus Type 5
TL;DR: Human embryonic kidney cells have been transformed by exposing cells to sheared fragments of adenovirus type 5 DNA, and the transformed cells exhibited many of the characteristics of transformation including the elaboration of a virus-specific tumour antigen.
Journal ArticleDOI
Vaccination with Irradiated Tumor Cells Engineered to Secrete Murine Granulocyte-Macrophage Colony-Stimulating Factor Stimulates Potent, Specific, and Long-Lasting Anti-Tumor Immunity
Glenn Dranoff,Elizabeth M. Jaffee,Audrey J. Lazenby,Paul Golumbek,Hyam I. Levitsky,Katja Brose,Valerie Jackson,Hirofumi Hamada,Drew M. Pardoll,Richard C. Mulligan +9 more
TL;DR: The results have important implications for the clinical use of genetically modified tumor cells as therapeutic cancer vaccines and the levels of anti-tumor immunity reported previously in cytokine gene transfer studies involving live, transduced cells could be achieved through the use of irradiated cells alone.
Journal ArticleDOI
Induction of chronic myelogenous leukemia in mice by the P210bcr/abl gene of the Philadelphia chromosome
TL;DR: It is demonstrated that P210bcr/abl expression can induce chronic myelogenous leukemia and retrovirus-mediated expression of the protein provides a murine model system for further analysis of the disease.
Journal ArticleDOI
The Basic Science of Gene Therapy
TL;DR: A large number of key technical issues need to be resolved before gene therapy can be safely and effectively applied in the clinic, and future technological developments will be critical for the successful practice of gene therapy.
Journal Article
Improved Retroviral Vectors for Gene Transfer and Expression
A D Miller,G J Rosman +1 more
TL;DR: A set of murine retrovirus-based vectors that include unique cloning sites for insertion of cDNAs such that the cDNA can be driven by either the retroviral long terminal repeat, the immediate early promoter of human cytomegalovirus, or the simian virus 40 early promoter are described.
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