PatentDOI
Production of high titer helper-free retroviruses by transient transfection
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TLDR
In this article, a method for producing high-titer, helper-free infectious retroviruses is disclosed which employs a novel strategy that uses transient transfection of new retroviral producer cell lines, ecotropic line BOSC 23 and amphotropic line CAK 8.Abstract:
A method for producing high-titer, helper-free infectious retroviruses is disclosed which employs a novel strategy that uses transient transfection of new retroviral producer cell lines, ecotropic line BOSC 23 and amphotropic line CAK 8, both of which cell lines and their precursor cell lines are disclosed. Because of the advantages over stable packaging cell lines, the BOSC 23 and CAK 8 transient transfection systems greatly facilitate and extend the use of helper-free retroviral vectors. The cell lines and corresponding methods possess wide application in both the medical and biotechnical fields, including gene therapy. These potential applications are disclosed and illustrated.read more
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Journal ArticleDOI
Heparan Sulfate Proteoglycans Mediate Attachment and Entry of Human T-Cell Leukemia Virus Type 1 Virions into CD4+ T Cells
TL;DR: HSPGs play a critical role in the binding and entry of HTLV-1 into CD4+ T cells, and blocking the interaction with HSPGs markedly reduced both the internalization of HT LV-1 virions and the titer of HTLD-1 pseudotyped viral infection in CD4- T cells.
Journal ArticleDOI
A family of transmembrane proteins with homology to the MET-hepatocyte growth factor receptor
Elena Maestrini,Luca Tamagnone,P Longati,Ottavio Cremona,Massimo Gulisano,Silvia Bione,Filippo Tamanini,Benjamin G. Neel,Daniela Toniolo,Paolo M. Comoglio +9 more
TL;DR: In this paper, a transmembrane protein (SEX) of 1871 amino acids was identified on Xq28 encoding a highly conserved sequence, which shares significant homology with the extracellular domain of the receptors encoded by the oncogenes MET, RON, and SEA [hepatocyte growth factor (HGF) receptor family].
Journal ArticleDOI
CD97, an adhesion receptor on inflammatory cells, stimulates angiogenesis through binding integrin counterreceptors on endothelial cells
Tao Wang,Yvona Ward,Linhua Tian,Ross Lake,Liliana Guedez,William G. Stetler-Stevenson,Kathleen Kelly +6 more
TL;DR: It was found that coengagement of alpha5 beta1 and chondroitotin sulfate proteoglycan by CD97 synergistically initiates endothelial cell invasion, and Integrin alpha5beta1 is the first high-affinity cellular counterreceptor that has been identified for a member within this family of adhesion receptors.
Journal ArticleDOI
Zinc-finger protein-targeted gene regulation: Genomewide single-gene specificity
Siyuan Tan,Dmitry Guschin,Albert R. Davalos,Ya-Li Lee,Andrew W. Snowden,Yann Jouvenot,H. Steven Zhang,Katherine Howes,Andrew R. McNamara,Albert Lai,Chris Ullman,Lindsey Reynolds,Michael J. Moore,Mark Isalan,Lutz-Peter Berg,Bradley Campos,Hong Qi,S. Kaye Spratt,Casey C. Case,Carl O. Pabo,Judith Campisi,Philip D. Gregory +21 more
TL;DR: It is reported that a ZFP TF can repress target gene expression with single-gene specificity within the human genome and was sufficient to generate a functional phenotype, as demonstrated by the loss of DNA damage-induced CHK2-dependent p53 phosphorylation.
Journal ArticleDOI
Distinct profiles of functional discrimination among G proteins determine the actions of G protein–coupled receptors
Ikuo Masuho,Olga Ostrovskaya,Grant M. Kramer,Grant M. Kramer,Christopher D. Jones,Christopher D. Jones,Keqiang Xie,Kirill A. Martemyanov +7 more
TL;DR: The observations suggest that the diversity of the effects of GPCRs on cellular physiology may be determined by their differential engagement of multiple G proteins, coupling to which produces signals with varying signal magnitudes and activation kinetics, properties that may be exploited pharmacologically.
References
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Vaccination with Irradiated Tumor Cells Engineered to Secrete Murine Granulocyte-Macrophage Colony-Stimulating Factor Stimulates Potent, Specific, and Long-Lasting Anti-Tumor Immunity
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Induction of chronic myelogenous leukemia in mice by the P210bcr/abl gene of the Philadelphia chromosome
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The Basic Science of Gene Therapy
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Journal Article
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