PatentDOI
Production of high titer helper-free retroviruses by transient transfection
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TLDR
In this article, a method for producing high-titer, helper-free infectious retroviruses is disclosed which employs a novel strategy that uses transient transfection of new retroviral producer cell lines, ecotropic line BOSC 23 and amphotropic line CAK 8.Abstract:
A method for producing high-titer, helper-free infectious retroviruses is disclosed which employs a novel strategy that uses transient transfection of new retroviral producer cell lines, ecotropic line BOSC 23 and amphotropic line CAK 8, both of which cell lines and their precursor cell lines are disclosed. Because of the advantages over stable packaging cell lines, the BOSC 23 and CAK 8 transient transfection systems greatly facilitate and extend the use of helper-free retroviral vectors. The cell lines and corresponding methods possess wide application in both the medical and biotechnical fields, including gene therapy. These potential applications are disclosed and illustrated.read more
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Journal ArticleDOI
Differential sialylation of cell surface glycoconjugates in a human B lymphoma cell line regulates susceptibility for CD95 (APO-1/Fas)-mediated apoptosis and for infection by a lymphotropic virus.
Oliver T. Keppler,Marcus E. Peter,Stephan Hinderlich,Gerhard Moldenhauer,Peer Stehling,Ingo Schmitz,Reinhard Schwartz-Albiez,Werner Reutter,Michael Pawlita +8 more
TL;DR: This is the first report demonstrating that differential sialylation in a clonal cell line may regulate the function of virus and signal-transducing receptors.
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Confluence of Vascular Endothelial Cells Induces Cell Cycle Exit by Inhibiting p42/p44 Mitogen-Activated Protein Kinase Activity
TL;DR: Investigation of the effect of cellular contact on the activation of p42/p44 mitogen-activated protein kinase (MAPK) by growth factors in mouse vascular endothelial cells suggests that phosphatases play a role in the down-regulation of p 42/ p44 MAPK activity.
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Highly efficient gene transfer into baboon marrow repopulating cells using GALV-pseudotype oncoretroviral vectors produced by human packaging cells
TL;DR: Transduction of human CD34(+) cells was significantly more efficient than transduction of baboon CD34 (+) cells, suggesting that Phoenix-GALV-derived oncoretroviral vectors may be even more efficient in human stem cell gene therapy applications.
Journal ArticleDOI
The cyclosporin A-sensitive nuclear factor of activated T cells (NFAT) proteins are expressed in vascular smooth muscle cells. Differential localization of NFAT isoforms and induction of NFAT-mediated transcription by phospholipase C-coupled cell surface receptors
TL;DR: C cultured vascular smooth muscle cells (VSMC) are shown to express two isoforms of the NFAT family endogenously, which are localized differentially in cells under resting conditions and indicate that agonists for phospholipase C-coupled receptors stimulate NFAT-mediated transcription in VSMC differentially, and that NFAT can function to integrate co-activating signals in the extracellular environment.
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γ-Catenin expression is reduced or absent in a subset of human lung cancers and re-expression inhibits transformed cell growth
Robert A. Winn,Robert A. Winn,Roy M. Bremnes,Lynne T. Bemis,Wilbur A. Franklin,York E. Miller,Carlyne D. Cool,Lynn E. Heasley +7 more
TL;DR: It is shown that γ-catenin can function as an inhibitor of β- catenin/TCF-dependent gene transcription and highlights γ/Catenin as a potentially novel tumor suppressor protein in a subset of human NSCLC cancers.
References
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Vaccination with Irradiated Tumor Cells Engineered to Secrete Murine Granulocyte-Macrophage Colony-Stimulating Factor Stimulates Potent, Specific, and Long-Lasting Anti-Tumor Immunity
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