PatentDOI
Production of high titer helper-free retroviruses by transient transfection
Reads0
Chats0
TLDR
In this article, a method for producing high-titer, helper-free infectious retroviruses is disclosed which employs a novel strategy that uses transient transfection of new retroviral producer cell lines, ecotropic line BOSC 23 and amphotropic line CAK 8.Abstract:
A method for producing high-titer, helper-free infectious retroviruses is disclosed which employs a novel strategy that uses transient transfection of new retroviral producer cell lines, ecotropic line BOSC 23 and amphotropic line CAK 8, both of which cell lines and their precursor cell lines are disclosed. Because of the advantages over stable packaging cell lines, the BOSC 23 and CAK 8 transient transfection systems greatly facilitate and extend the use of helper-free retroviral vectors. The cell lines and corresponding methods possess wide application in both the medical and biotechnical fields, including gene therapy. These potential applications are disclosed and illustrated.read more
Citations
More filters
Journal ArticleDOI
Regulation of synapsin I gene expression by the zinc finger transcription factor zif268/egr-1.
TL;DR: It is reported that the zif268/egr-1 protein bound in vitro to two sites in the proximal regulatory region of the human synapsin I gene, which concludes that the synapsIn I gene is a target of the zIF268 transcription factor; however, intermediate factors may also be involved in the activation.
Journal ArticleDOI
The cytostatic function of c-Abl is controlled by multiple nuclear localization signals and requires the p53 and Rb tumor suppressor gene products.
TL;DR: The results indicate that Abl inhibits cell proliferation by interacting with central elements of the cell cycle control apparatus in the nucleus, and suggest a direct connection between p53 and Rb in this growth‐inhibitory pathway.
Journal ArticleDOI
Cell Cycle-Dependent Phosphorylation of Mammalian Protein Phosphatase 1 by cdc2 Kinase
TL;DR: Observations suggest that phosphorylation by cdc2 kinase in early to mid-mitosis and inhibition of PP-1 activity is likely to contribute to the increased state of phosphorylated of proteins that is critical to the initiation of normal cell division.
Journal ArticleDOI
The CATERPILLER Protein Monarch-1 Is an Antagonist of Toll-like Receptor-, Tumor Necrosis Factor α-, and Mycobacterium tuberculosis-induced Pro-inflammatory Signals
Kristi L. Williams,John D. Lich,Joseph A. Duncan,William Reed,Prasad Rallabhandi,Chris B. Moore,Sherry Kurtz,V. McNeil Coffield,Mary Ann Accavitti-Loper,Lishan Su,Stefanie N. Vogel,Miriam Braunstein,Jenny P.-Y. Ting +12 more
TL;DR: This work finds that Monarch-1 associates with IRAK-1 but not MyD88, resulting in the blockage of IRAk-1 hyperphosphorylation, the first example of a CLR protein that antagonizes inflammatory responses initiated by TLR agonists via interference with IRAB-1 activation.
Journal ArticleDOI
Novel modulation of neuronal nicotinic acetylcholine receptors by association with the endogenous prototoxin lynx1.
Inés Ibañez-Tallon,Julie M. Miwa,Hai Long Wang,Niels C. Adams,Gregg W. Crabtree,Steven M. Sine,Nathaniel Heintz +6 more
TL;DR: It is established that direct interaction of lynx1 with nAChRs can result in a novel type of functional modulation and suggest that prototoxins may play important roles in vivo by modulating functional properties of their cognate CNS receptors.
References
More filters
Journal ArticleDOI
Characteristics of a Human Cell Line Transformed by DNA from Human Adenovirus Type 5
TL;DR: Human embryonic kidney cells have been transformed by exposing cells to sheared fragments of adenovirus type 5 DNA, and the transformed cells exhibited many of the characteristics of transformation including the elaboration of a virus-specific tumour antigen.
Journal ArticleDOI
Vaccination with Irradiated Tumor Cells Engineered to Secrete Murine Granulocyte-Macrophage Colony-Stimulating Factor Stimulates Potent, Specific, and Long-Lasting Anti-Tumor Immunity
Glenn Dranoff,Elizabeth M. Jaffee,Audrey J. Lazenby,Paul Golumbek,Hyam I. Levitsky,Katja Brose,Valerie Jackson,Hirofumi Hamada,Drew M. Pardoll,Richard C. Mulligan +9 more
TL;DR: The results have important implications for the clinical use of genetically modified tumor cells as therapeutic cancer vaccines and the levels of anti-tumor immunity reported previously in cytokine gene transfer studies involving live, transduced cells could be achieved through the use of irradiated cells alone.
Journal ArticleDOI
Induction of chronic myelogenous leukemia in mice by the P210bcr/abl gene of the Philadelphia chromosome
TL;DR: It is demonstrated that P210bcr/abl expression can induce chronic myelogenous leukemia and retrovirus-mediated expression of the protein provides a murine model system for further analysis of the disease.
Journal ArticleDOI
The Basic Science of Gene Therapy
TL;DR: A large number of key technical issues need to be resolved before gene therapy can be safely and effectively applied in the clinic, and future technological developments will be critical for the successful practice of gene therapy.
Journal Article
Improved Retroviral Vectors for Gene Transfer and Expression
A D Miller,G J Rosman +1 more
TL;DR: A set of murine retrovirus-based vectors that include unique cloning sites for insertion of cDNAs such that the cDNA can be driven by either the retroviral long terminal repeat, the immediate early promoter of human cytomegalovirus, or the simian virus 40 early promoter are described.
Related Papers (5)
Advanced mammalian gene transfer: high titre retroviral vectors with multiple drug selection markers and a complementary helper-free packaging cell line
Jay P. Morgenstern,Hartmut Land +1 more