PatentDOI
Production of high titer helper-free retroviruses by transient transfection
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TLDR
In this article, a method for producing high-titer, helper-free infectious retroviruses is disclosed which employs a novel strategy that uses transient transfection of new retroviral producer cell lines, ecotropic line BOSC 23 and amphotropic line CAK 8.Abstract:
A method for producing high-titer, helper-free infectious retroviruses is disclosed which employs a novel strategy that uses transient transfection of new retroviral producer cell lines, ecotropic line BOSC 23 and amphotropic line CAK 8, both of which cell lines and their precursor cell lines are disclosed. Because of the advantages over stable packaging cell lines, the BOSC 23 and CAK 8 transient transfection systems greatly facilitate and extend the use of helper-free retroviral vectors. The cell lines and corresponding methods possess wide application in both the medical and biotechnical fields, including gene therapy. These potential applications are disclosed and illustrated.read more
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Construction and characterization of retroviral vectors expressing biologically active human interleukin-12
Laurence Zitvogel,Hideaki Tahara,Quan Cai,Walter J. Storkus,Gunhild Muller,Stanley F. Wolf,Maurice Gately,Paul D. Robbins,Michael T. Lotze +8 more
TL;DR: These retroviral vectors expressing human IL- 12 should be useful in evaluating the biological properties of IL-12 as well as for use in clinical trials for gene therapy of patients with cancer.
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Fibroblast Growth Factor-2 Induces Translational Regulation of Bcl-XL and Bcl-2 via a MEK-dependent Pathway
Olivier E. Pardo,Alexandre Arcaro,Giovanni Salerno,Selina Raguz,Julian Downward,Michael J. Seckl +5 more
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Characterization of KIF1C, a New Kinesin-like Protein Involved in Vesicle Transport from the Golgi Apparatus to the Endoplasmic Reticulum
TL;DR: The data suggest an involvement of tyrosine phosphorylation in the regulation of the Golgi to ER membrane flow and describe a new kinesin-like motor protein responsible for this transport.
Journal ArticleDOI
Mitotic chromosome condensation mediated by the retinoblastoma protein is tumor-suppressive
Courtney H. Coschi,Alison L. Martens,Kieran Ritchie,Sarah M. Francis,Subrata Chakrabarti,Nathalie G. Bérubé,Frederick A. Dick +6 more
TL;DR: It is demonstrated that defective mitotic chromosome structure caused by mutant pRB accelerates loss of heterozygosity, leading to earlier tumor formation in Trp53(+/-) mice, revealing a new mechanism of tumor suppression, facilitated by pRB, in which genome stability is maintained by proper condensation of mitotic chromosomes.
Journal ArticleDOI
Induction of beta3-integrin gene expression by sustained activation of the Ras-regulated Raf-MEK-extracellular signal-regulated kinase signaling pathway.
Douglas Woods,Holly Cherwinski,Eleni Venetsanakos,Arun Bhat,Stephan Gysin,Martine Humbert,Paul F. Bray,Vicki L. Saylor,Martin McMahon +8 more
TL;DR: It is demonstrated that the Ras-activated Raf–MEK–extracellular signal-regulated kinase (ERK) signaling pathway can specifically control the expression of individual integrin subunits in a variety of human and mouse cell lines, and oncogene-induced alterations in integrin gene expression may participate in the changes in cell adhesion and migration that accompany the process of oncogenic transformation.
References
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Vaccination with Irradiated Tumor Cells Engineered to Secrete Murine Granulocyte-Macrophage Colony-Stimulating Factor Stimulates Potent, Specific, and Long-Lasting Anti-Tumor Immunity
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The Basic Science of Gene Therapy
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Improved Retroviral Vectors for Gene Transfer and Expression
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