PatentDOI
Production of high titer helper-free retroviruses by transient transfection
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TLDR
In this article, a method for producing high-titer, helper-free infectious retroviruses is disclosed which employs a novel strategy that uses transient transfection of new retroviral producer cell lines, ecotropic line BOSC 23 and amphotropic line CAK 8.Abstract:
A method for producing high-titer, helper-free infectious retroviruses is disclosed which employs a novel strategy that uses transient transfection of new retroviral producer cell lines, ecotropic line BOSC 23 and amphotropic line CAK 8, both of which cell lines and their precursor cell lines are disclosed. Because of the advantages over stable packaging cell lines, the BOSC 23 and CAK 8 transient transfection systems greatly facilitate and extend the use of helper-free retroviral vectors. The cell lines and corresponding methods possess wide application in both the medical and biotechnical fields, including gene therapy. These potential applications are disclosed and illustrated.read more
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Oct3/4, Klf4, c-Myc and Sox2 produce induced pluripotent stem cells
TL;DR: In this article, a nuclear reprogramming factor has been used to reprogram a differentiated somatic cell to derive an induced pluripotent stem (iPS) cell, which can then be used for stem cell therapy.
Journal ArticleDOI
Murine Stat2 is uncharacteristically divergent
TL;DR: Proteins that interact with the C-termini of murine and human Stat2 interact with an overlapping, but distinct set of proteins, suggesting that murine Stat2 may have evolved to mediate some unique functions as well.
Journal ArticleDOI
TBX15 mutations cause craniofacial dysmorphism, hypoplasia of scapula and pelvis, and short stature in Cousin syndrome.
Ekkehart Lausch,Pia Hermanns,Henner F. Farin,Yasemin Alanay,Sheila Unger,Sarah Nikkel,Sarah Nikkel,Christoph Steinwender,Gerd Scherer,Jürgen W. Spranger,Bernhard Zabel,Andreas Kispert,Andrea Superti-Furga +12 more
TL;DR: It is concluded that Cousin syndrome is caused by TBX15 insufficiency and is thus the human counterpart of the droopy ear mouse.
Journal ArticleDOI
Enforced expression of the GATA-3 transcription factor affects cell fate decisions in hematopoiesis.
TL;DR: The selective megakaryocytic/erythroid differentiation in HSC with enforced GATA-3 expression suggests a functional redundancy among GATA proteins and indicates that the specific lineage fate determination by individual Gata proteins is largely regulated at the level of expression in a lineage and developmental-stage restricted fashion.
Journal ArticleDOI
Development of Amphotropic Murine Retrovirus Vectors Resistant to Inactivation by Human Serum
TL;DR: Surprisingly, while TE671 cells were resistant, TE671-derived RV were sensitive to inactivation, and these observations have important implications for use of RV and RV-producer cells for human gene therapy.
References
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Journal ArticleDOI
Characteristics of a Human Cell Line Transformed by DNA from Human Adenovirus Type 5
TL;DR: Human embryonic kidney cells have been transformed by exposing cells to sheared fragments of adenovirus type 5 DNA, and the transformed cells exhibited many of the characteristics of transformation including the elaboration of a virus-specific tumour antigen.
Journal ArticleDOI
Vaccination with Irradiated Tumor Cells Engineered to Secrete Murine Granulocyte-Macrophage Colony-Stimulating Factor Stimulates Potent, Specific, and Long-Lasting Anti-Tumor Immunity
Glenn Dranoff,Elizabeth M. Jaffee,Audrey J. Lazenby,Paul Golumbek,Hyam I. Levitsky,Katja Brose,Valerie Jackson,Hirofumi Hamada,Drew M. Pardoll,Richard C. Mulligan +9 more
TL;DR: The results have important implications for the clinical use of genetically modified tumor cells as therapeutic cancer vaccines and the levels of anti-tumor immunity reported previously in cytokine gene transfer studies involving live, transduced cells could be achieved through the use of irradiated cells alone.
Journal ArticleDOI
Induction of chronic myelogenous leukemia in mice by the P210bcr/abl gene of the Philadelphia chromosome
TL;DR: It is demonstrated that P210bcr/abl expression can induce chronic myelogenous leukemia and retrovirus-mediated expression of the protein provides a murine model system for further analysis of the disease.
Journal ArticleDOI
The Basic Science of Gene Therapy
TL;DR: A large number of key technical issues need to be resolved before gene therapy can be safely and effectively applied in the clinic, and future technological developments will be critical for the successful practice of gene therapy.
Journal Article
Improved Retroviral Vectors for Gene Transfer and Expression
A D Miller,G J Rosman +1 more
TL;DR: A set of murine retrovirus-based vectors that include unique cloning sites for insertion of cDNAs such that the cDNA can be driven by either the retroviral long terminal repeat, the immediate early promoter of human cytomegalovirus, or the simian virus 40 early promoter are described.
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