PatentDOI
Production of high titer helper-free retroviruses by transient transfection
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TLDR
In this article, a method for producing high-titer, helper-free infectious retroviruses is disclosed which employs a novel strategy that uses transient transfection of new retroviral producer cell lines, ecotropic line BOSC 23 and amphotropic line CAK 8.Abstract:
A method for producing high-titer, helper-free infectious retroviruses is disclosed which employs a novel strategy that uses transient transfection of new retroviral producer cell lines, ecotropic line BOSC 23 and amphotropic line CAK 8, both of which cell lines and their precursor cell lines are disclosed. Because of the advantages over stable packaging cell lines, the BOSC 23 and CAK 8 transient transfection systems greatly facilitate and extend the use of helper-free retroviral vectors. The cell lines and corresponding methods possess wide application in both the medical and biotechnical fields, including gene therapy. These potential applications are disclosed and illustrated.read more
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Journal ArticleDOI
Intercellular delivery of thymidine kinase prodrug activating enzyme by the herpes simplex virus protein, VP22
M. S. Dilber,Anne Phelan,Alar Aints,Abdalla J. Mohamed,Gillian Elliott,C. I. E. Smith,Peter O'Hare +6 more
TL;DR: It is demonstrated that fusion proteins consisting of the herpes simplex virus (HSV) transport protein VP22 linked in frame to HSV thymidine kinase (tk) retain the ability to be transported between cells.
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HER4 Mediates Ligand-Dependent Antiproliferative and Differentiation Responses in Human Breast Cancer Cells
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The E6 and E7 Proteins of the Cutaneous Human Papillomavirus Type 38 Display Transforming Properties
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Janus kinases affect thrombopoietin receptor cell surface localization and stability.
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High level calcineurin activity predisposes neuronal cells to apoptosis.
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TL;DR: The data indicate that high level constitutive activity of calcineurin predisposes neuronal cells to cytochrome c/caspase-3 dependent apoptosis even under sublethal conditions.
References
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Characteristics of a Human Cell Line Transformed by DNA from Human Adenovirus Type 5
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Vaccination with Irradiated Tumor Cells Engineered to Secrete Murine Granulocyte-Macrophage Colony-Stimulating Factor Stimulates Potent, Specific, and Long-Lasting Anti-Tumor Immunity
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Journal ArticleDOI
The Basic Science of Gene Therapy
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Journal Article
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