PatentDOI
Production of high titer helper-free retroviruses by transient transfection
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TLDR
In this article, a method for producing high-titer, helper-free infectious retroviruses is disclosed which employs a novel strategy that uses transient transfection of new retroviral producer cell lines, ecotropic line BOSC 23 and amphotropic line CAK 8.Abstract:
A method for producing high-titer, helper-free infectious retroviruses is disclosed which employs a novel strategy that uses transient transfection of new retroviral producer cell lines, ecotropic line BOSC 23 and amphotropic line CAK 8, both of which cell lines and their precursor cell lines are disclosed. Because of the advantages over stable packaging cell lines, the BOSC 23 and CAK 8 transient transfection systems greatly facilitate and extend the use of helper-free retroviral vectors. The cell lines and corresponding methods possess wide application in both the medical and biotechnical fields, including gene therapy. These potential applications are disclosed and illustrated.read more
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Lateral transport of Smoothened from the plasma membrane to the membrane of the cilium.
TL;DR: A distinct pathway for ligand-stimulated lateral trafficking of the hedgehog protein Smoothened into cilia is described.
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VDUP1 upregulated by TGF-β1 and 1,25-dihydorxyvitamin D3 inhibits tumor cell growth by blocking cell-cycle progression
Seung Hyun Han,Jun Ho Jeon,Hyang Ran Ju,Uhee Jung,Kun Young Kim,Hyang Sook Yoo,Young Ho Lee,Kyu Sang Song,Ho Myeung Hwang,Yoon Sook Na,Young Yang,Kee Nyung Lee,Inpyo Choi +12 more
TL;DR: Results suggest that VDUP1 is a novel antitumor gene which forms a transcriptional repressor complex which possesses a tumor-suppressive activity.
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Pregnancy-associated plasma protein-A (PAPP-A) cleaves insulin-like growth factor binding protein (IGFBP)-5 independent of IGF: implications for the mechanism of IGFBP-4 proteolysis by PAPP-A.
Lisbeth S. Laursen,Michael Toft Overgaard,Rikke Søe,Henning B. Boldt,Lars Sottrup-Jensen,Linda C. Giudice,Cheryl A. Conover,Claus Oxvig +7 more
TL;DR: It is concluded that IGF enhances proteolysis by binding to IGFBP‐4, not by interaction with PAPP‐A, which could not previously be ruled out.
Journal ArticleDOI
Involvement of the IRF family transcription factor IRF‐3 in virus‐induced activation of the IFN‐β gene
TL;DR: It is shown that IRF‐3, an IRF family transcription factor, translocates to the nucleus from the cytoplasm upon virus infection in NIH/3T3 cells and is a candidate transcription factor mediating the activation of the IFN‐β gene.
Journal ArticleDOI
FOG‐1 recruits the NuRD repressor complex to mediate transcriptional repression by GATA‐1
Wei Hong,Minako Nakazawa,Ying-Yu Chen,Rajashree Kori,Christopher R. Vakoc,Christopher R. Vakoc,Carrie Rakowski,Gerd A. Blobel,Gerd A. Blobel +8 more
TL;DR: FOG‐1 and likely other FOG‐like proteins are corepressors that link GATA factors to histone deacetylation and nucleosome remodeling and the ability of GATA‐1 to repress transcription was impaired in erythroid cells expressing mutant forms of FOG•1 that are defective for NuRD binding.
References
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Characteristics of a Human Cell Line Transformed by DNA from Human Adenovirus Type 5
TL;DR: Human embryonic kidney cells have been transformed by exposing cells to sheared fragments of adenovirus type 5 DNA, and the transformed cells exhibited many of the characteristics of transformation including the elaboration of a virus-specific tumour antigen.
Journal ArticleDOI
Vaccination with Irradiated Tumor Cells Engineered to Secrete Murine Granulocyte-Macrophage Colony-Stimulating Factor Stimulates Potent, Specific, and Long-Lasting Anti-Tumor Immunity
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Induction of chronic myelogenous leukemia in mice by the P210bcr/abl gene of the Philadelphia chromosome
TL;DR: It is demonstrated that P210bcr/abl expression can induce chronic myelogenous leukemia and retrovirus-mediated expression of the protein provides a murine model system for further analysis of the disease.
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The Basic Science of Gene Therapy
TL;DR: A large number of key technical issues need to be resolved before gene therapy can be safely and effectively applied in the clinic, and future technological developments will be critical for the successful practice of gene therapy.
Journal Article
Improved Retroviral Vectors for Gene Transfer and Expression
A D Miller,G J Rosman +1 more
TL;DR: A set of murine retrovirus-based vectors that include unique cloning sites for insertion of cDNAs such that the cDNA can be driven by either the retroviral long terminal repeat, the immediate early promoter of human cytomegalovirus, or the simian virus 40 early promoter are described.
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