PatentDOI
Production of high titer helper-free retroviruses by transient transfection
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TLDR
In this article, a method for producing high-titer, helper-free infectious retroviruses is disclosed which employs a novel strategy that uses transient transfection of new retroviral producer cell lines, ecotropic line BOSC 23 and amphotropic line CAK 8.Abstract:
A method for producing high-titer, helper-free infectious retroviruses is disclosed which employs a novel strategy that uses transient transfection of new retroviral producer cell lines, ecotropic line BOSC 23 and amphotropic line CAK 8, both of which cell lines and their precursor cell lines are disclosed. Because of the advantages over stable packaging cell lines, the BOSC 23 and CAK 8 transient transfection systems greatly facilitate and extend the use of helper-free retroviral vectors. The cell lines and corresponding methods possess wide application in both the medical and biotechnical fields, including gene therapy. These potential applications are disclosed and illustrated.read more
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Requirement for the CD95 Receptor-Ligand Pathway in c-Myc-Induced Apoptosis
Anne-Odile Hueber,Martin Zörnig,Martin Zörnig,Debbie Lyon,Debbie Lyon,Takashi Suda,Takashi Suda,Shigekazu Nagata,Gerard I. Evan,Gerard I. Evan +9 more
TL;DR: Findings link two apoptotic pathways previously thought to be independent and establish the dependency of Myc on CD95 signaling for its killing activity and suppress c-myc-induced apoptosis by also acting downstream of CD95.
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Interleukin 3-dependent survival by the Akt protein kinase
TL;DR: It is found that Akt kinase activity is rapidly induced by the cytokine IL-3, suggesting a role for Akt in PI 3-kinase-dependent signaling in hematopoetic cells and appears to be an important mediator of anti-apoptotic signaling in this system.
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Role of p53 and p21waf1/cip1 in senescence-like terminal proliferation arrest induced in human tumor cells by chemotherapeutic drugs.
Bey-Dih Chang,Yongzhi Xuan,Eugenia V. Broude,Hongming Zhu,Brigitte Schott,Jing Fang,Igor B. Roninson +6 more
TL;DR: The results indicate that p53 and p21 act as positive regulators of senescence-like terminal proliferation arrest, but their function is neither sufficient nor absolutely required for this treatment response in tumor cells.
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Plasma membrane-associated proteins are clustered into islands attached to the cytoskeleton.
TL;DR: Transmission electron microscopy of plasma membrane sheets and specific probes are used to show that most or all plasma membrane-associated proteins are clustered in cholesterol-enriched domains (“islands”) that are separated by “protein-free” and cholesterol-low membrane.
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Transcription factor Foxp3 and its protein partners form a complex regulatory network
Dipayan Rudra,Paul deRoos,Ashutosh Chaudhry,Rachel E. Niec,Aaron Arvey,Aaron Arvey,Robert M. Samstein,Christina S. Leslie,Scott A. Shaffer,Scott A. Shaffer,David R. Goodlett,Alexander Y. Rudensky +11 more
TL;DR: Functional analysis of the cooperation of Foxp3 with one such partner, GATA-3, provided additional evidence for a network of transcriptional regulation afforded byFoxp3 and its associates to control distinct aspects of Treg cell biology.
References
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Vaccination with Irradiated Tumor Cells Engineered to Secrete Murine Granulocyte-Macrophage Colony-Stimulating Factor Stimulates Potent, Specific, and Long-Lasting Anti-Tumor Immunity
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