PatentDOI
Production of high titer helper-free retroviruses by transient transfection
Reads0
Chats0
TLDR
In this article, a method for producing high-titer, helper-free infectious retroviruses is disclosed which employs a novel strategy that uses transient transfection of new retroviral producer cell lines, ecotropic line BOSC 23 and amphotropic line CAK 8.Abstract:
A method for producing high-titer, helper-free infectious retroviruses is disclosed which employs a novel strategy that uses transient transfection of new retroviral producer cell lines, ecotropic line BOSC 23 and amphotropic line CAK 8, both of which cell lines and their precursor cell lines are disclosed. Because of the advantages over stable packaging cell lines, the BOSC 23 and CAK 8 transient transfection systems greatly facilitate and extend the use of helper-free retroviral vectors. The cell lines and corresponding methods possess wide application in both the medical and biotechnical fields, including gene therapy. These potential applications are disclosed and illustrated.read more
Citations
More filters
Journal ArticleDOI
In Vivo Gene Delivery and Stable Transduction of Nondividing Cells by a Lentiviral Vector
Luigi Naldini,Ulrike Blömer,Philippe Gallay,Daniel S. Ory,Richard C. Mulligan,Fred H. Gage,Inder M. Verma,Didier Trono +7 more
TL;DR: The ability of HIV-based viral vectors to deliver genes in vivo into nondividing cells could increase the applicability of retroviral vectors in human gene therapy.
Journal ArticleDOI
Identification of a major co-receptor for primary isolates of HIV-1
Hongkui Deng,Rong Liu,Wilfried Ellmeier,S Choe,Derya Unutmaz,M Burkhart,P Di Marzio,Shoshana Marmon,R E Sutton,C M Hill,C B Davis,S C Peiper,T J Schall,Dan R. Littman,Nathaniel R. Landau +14 more
TL;DR: The principal cofactor for entry mediated by the envelope glycoproteins of primary macrophage-tropic strains of HIV-1 is CC-CKR-5, a receptor for the β-chemokines RANTES, Mip-1α and MIP-1β.
Journal ArticleDOI
A cold-inducible coactivator of nuclear receptors linked to adaptive thermogenesis.
Pere Puigserver,Zhidan Wu,Cheol Won Park,Reed A. Graves,Margaret E. Wright,Bruce M. Spiegelman +5 more
TL;DR: Results indicate that PGC-1 plays a key role in linking nuclear receptors to the transcriptional program of adaptive thermogenesis.
Journal ArticleDOI
β‐catenin is a target for the ubiquitin–proteasome pathway
TL;DR: It is shown that ubiquitination of β‐catenin is greatly reduced in Wnt‐expressing cells, providing the first evidence that the ubiquitin–proteasome degradation pathway may act downstream of GSK3β in the regulation ofβ‐ catenin.
Journal ArticleDOI
Hypoxia-mediated selection of cells with diminished apoptotic potential in solid tumours
Thomas G. Graeber,Cynthia Osmanian,Tyler Jacks,David E. Housman,Cameron J. Koch,Scott W. Lowe,Scott W. Lowe,Amato J. Giaccia +7 more
TL;DR: It is proposed that hypoxia provides a physiological selective pressure in tumours for the expansion of variants that have lost their apoptotic potential, and in particular for cells acquiring p53mutations.
References
More filters
Journal ArticleDOI
Intramolecular integration within Moloney murine leukemia virus DNA.
TL;DR: Screening a library of unintegrated, circular Moloney murine leukemia virus (M-MuLV) DNA cloned in lambda phage found that approximately 20% of the M- MuLV DNA inserts contained internal sequence deletions or inversions, suggesting that many of the variants arose as a consequence of M-muLV DNA molecules integrating within their own DNA.
Journal ArticleDOI
Use of simian virus 40 replication to amplify Epstein-Barr virus shuttle vectors in human cells.
TL;DR: It was determined that SV40 replication generates a detectable increase in the deletion frequency but no measurable increaseIn the frequency of point mutations, and a high frequency of intramolecular recombination when the vector carried a repeat segment in direct orientation, but not when the repeat was in inverted orientation or absent.
Journal ArticleDOI
Lethal effect of the Abelson murine leukemia virus transforming gene product.
TL;DR: It is suspected that the carboxy-terminal portion of the wild-type Abelson protein may modulate the in vivo expression of the protein kinase activity and that this may cause the transforming protein to have a deleterious effect on certain cell lines.
Journal ArticleDOI
Enrichment of murine haemopoietic stem cells: diverging roads
TL;DR: In this review, Gerald Spangrude examines several experimental approaches that have been used to enrich murine haemopoietic stem cells and concludes that in-vitro culture systems should be developed to maintain these rare stem cells.
Journal ArticleDOI
Construction of a fusion gene that confers resistance against hygromycin B to mammalian cells in culture
TL;DR: The gene conferring resistance against hygromycin B in E. coli is modified in such a way that it can be transcribed in mammalian cells from the promoter of the HSVtk gene and the resulting plasmid, pHMR272, is a useful addition to the relatively small number of dominant selectable markers available for mammalian cells.
Related Papers (5)
Advanced mammalian gene transfer: high titre retroviral vectors with multiple drug selection markers and a complementary helper-free packaging cell line
Jay P. Morgenstern,Hartmut Land +1 more