The Largest Group of Superficial Neocortical GABAergic Interneurons Expresses Ionotropic Serotonin Receptors
TLDR
Fast modulation of serotonergic and cholinergic transmission may influence cortical activity through an enhancement of GABAergic synaptic transmission from 5-HT3AR-expressing neurons during sensory process depending on different behavioral states.Abstract:
A highly diverse population of neocortical GABAergic inhibitory interneurons has been implicated in multiple functions in information processing within cortical circuits. The diversity of cortical interneurons is determined during development and primarily depends on their embryonic origins either from the medial (MGE) or the caudal (CGE) ganglionic eminences. Although MGE-derived parvalbumin (PV)- or somatostatin (SST)-expressing interneurons are well characterized, less is known about the other types of cortical GABAergic interneurons, especially those of CGE lineage, because of the lack of specific neuronal markers for these interneuron subtypes. Using a bacterial artificial chromosome transgenic mouse line, we show that, in the somatosensory cortex of the mouse, the serotonin 5-hydroxytryptamine 3A (5-HT(3A)) receptor, the only ionotropic serotonergic receptor, is expressed in most, if not all, neocortical GABAergic interneurons that do not express PV or SST. Genetic fate mapping and neurochemical profile demonstrate that 5-HT(3A)R-expressing neurons include the entire spectrum of CGE-derived interneurons. We report that, in addition to serotonergic responsiveness via 5-HT(3A)Rs, acetylcholine also depolarizes 5-HT(3A)R-expressing neurons via nicotinic receptors. 5-HT(3A)R-expressing neurons in thalamocortical (TC) recipient areas receive weak but direct monosynaptic inputs from the thalamus. TC input depolarizes a subset of TC-recipient 5-HT(3A)R neurons as strongly as fast-spiking cells, in part because of their high input resistance. Hence, fast modulation of serotonergic and cholinergic transmission may influence cortical activity through an enhancement of GABAergic synaptic transmission from 5-HT(3A)R-expressing neurons during sensory process depending on different behavioral states.read more
Citations
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Cell types in the mouse cortex and hippocampus revealed by single-cell RNA-seq
Amit Zeisel,Ana B. Muñoz-Manchado,Simone Codeluppi,Peter Lönnerberg,Gioele La Manno,Anna Juréus,Sueli Marques,Hermany Munguba,Liqun He,Christer Betsholtz,Christer Betsholtz,Charlotte Rolny,Gonçalo Castelo-Branco,Jens Hjerling-Leffler,Sten Linnarsson +14 more
TL;DR: Large-scale single-cell RNA sequencing is used to classify cells in the mouse somatosensory cortex and hippocampal CA1 region and found 47 molecularly distinct subclasses, comprising all known major cell types in the cortex.
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GABAergic Interneurons in the Neocortex: From Cellular Properties to Circuits
TL;DR: Current understanding of neocortical interneuron diversity and the properties that distinguish cell types are reviewed and it is illustrated how recent advances in the field have shed light onto the mechanisms by which GABAergic inhibition contributes to network operations.
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Three groups of interneurons account for nearly 100% of neocortical GABAergic neurons
TL;DR: The universal modulation of these neurons by serotonin and acetylcholine via ionotropic receptors suggests that they might be involved in shaping cortical circuits during specific brain states andbehavioral contexts.
Journal ArticleDOI
Inhibition of Inhibition in Visual Cortex: The Logic of Connections Between Molecularly Distinct Interneurons
TL;DR: This work describes a simple and complementary interaction scheme between three large, molecularly distinct interneuron populations in mouse visual cortex: parvalbumin-expressing interneurons strongly inhibit one another but provide little inhibition to other populations, while somatostatin-expresses avoid inhibiting one another yet strongly inhibit all other populations.
Journal ArticleDOI
Conserved cell types with divergent features in human versus mouse cortex.
Rebecca D. Hodge,Trygve E. Bakken,Jeremy A. Miller,Kimberly A. Smith,Eliza Barkan,Lucas T. Graybuck,Jennie L. Close,Brian Long,Nelson Johansen,Osnat Penn,Zizhen Yao,Jeroen Eggermont,Thomas Höllt,Thomas Höllt,Boaz P. Levi,Soraya I. Shehata,Brian D. Aevermann,Allison Beller,Darren Bertagnolli,Krissy Brouner,Tamara Casper,Charles Cobbs,Rachel A. Dalley,Nick Dee,Songlin Ding,Richard G. Ellenbogen,Olivia Fong,Emma Garren,Jeff Goldy,Ryder P. Gwinn,Daniel Hirschstein,C. Dirk Keene,Mohamed Keshk,Andrew L. Ko,Andrew L. Ko,Kanan Lathia,Ahmed Mahfouz,Ahmed Mahfouz,Zoe Maltzer,Medea McGraw,Thuc Nghi Nguyen,Julie Nyhus,Jeffrey G. Ojemann,Jeffrey G. Ojemann,Aaron Oldre,Sheana Parry,Shannon Reynolds,Christine Rimorin,Nadiya V. Shapovalova,Saroja Somasundaram,Aaron Szafer,Elliot R. Thomsen,Michael Tieu,Gerald Quon,Richard H. Scheuermann,Richard H. Scheuermann,Rafael Yuste,Susan M. Sunkin,Boudewijn P. F. Lelieveldt,Boudewijn P. F. Lelieveldt,David Feng,Lydia Ng,Amy Bernard,Michael Hawrylycz,John W. Phillips,Bosiljka Tasic,Hongkui Zeng,Allan R. Jones,Christof Koch,Ed Lein +69 more
TL;DR: RNA-sequencing analysis of cells in the human cortex enabled identification of diverse cell types, revealing well-conserved architecture and homologous cell types as well as extensive differences when compared with datasets covering the analogous region of the mouse brain.
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