Conserved cell types with divergent features in human versus mouse cortex.
Rebecca D. Hodge,Trygve E. Bakken,Jeremy A. Miller,Kimberly A. Smith,Eliza Barkan,Lucas T. Graybuck,Jennie L. Close,Brian Long,Nelson Johansen,Osnat Penn,Zizhen Yao,Jeroen Eggermont,Thomas Höllt,Thomas Höllt,Boaz P. Levi,Soraya I. Shehata,Brian D. Aevermann,Allison Beller,Darren Bertagnolli,Krissy Brouner,Tamara Casper,Charles Cobbs,Rachel A. Dalley,Nick Dee,Songlin Ding,Richard G. Ellenbogen,Olivia Fong,Emma Garren,Jeff Goldy,Ryder P. Gwinn,Daniel Hirschstein,C. Dirk Keene,Mohamed Keshk,Andrew L. Ko,Andrew L. Ko,Kanan Lathia,Ahmed Mahfouz,Ahmed Mahfouz,Zoe Maltzer,Medea McGraw,Thuc Nghi Nguyen,Julie Nyhus,Jeffrey G. Ojemann,Jeffrey G. Ojemann,Aaron Oldre,Sheana Parry,Shannon Reynolds,Christine Rimorin,Nadiya V. Shapovalova,Saroja Somasundaram,Aaron Szafer,Elliot R. Thomsen,Michael Tieu,Gerald Quon,Richard H. Scheuermann,Richard H. Scheuermann,Rafael Yuste,Susan M. Sunkin,Boudewijn P. F. Lelieveldt,Boudewijn P. F. Lelieveldt,David Feng,Lydia Ng,Amy Bernard,Michael Hawrylycz,John W. Phillips,Bosiljka Tasic,Hongkui Zeng,Allan R. Jones,Christof Koch,Ed Lein +69 more
TLDR
RNA-sequencing analysis of cells in the human cortex enabled identification of diverse cell types, revealing well-conserved architecture and homologous cell types as well as extensive differences when compared with datasets covering the analogous region of the mouse brain.Abstract:
Elucidating the cellular architecture of the human cerebral cortex is central to understanding our cognitive abilities and susceptibility to disease. Here we used single-nucleus RNA-sequencing analysis to perform a comprehensive study of cell types in the middle temporal gyrus of human cortex. We identified a highly diverse set of excitatory and inhibitory neuron types that are mostly sparse, with excitatory types being less layer-restricted than expected. Comparison to similar mouse cortex single-cell RNA-sequencing datasets revealed a surprisingly well-conserved cellular architecture that enables matching of homologous types and predictions of properties of human cell types. Despite this general conservation, we also found extensive differences between homologous human and mouse cell types, including marked alterations in proportions, laminar distributions, gene expression and morphology. These species-specific features emphasize the importance of directly studying human brain.read more
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Construction of a human cell landscape at single-cell level.
Xiaoping Han,Ziming Zhou,Lijiang Fei,Huiyu Sun,Renying Wang,Yao Chen,Haide Chen,Jingjing Wang,Huanna Tang,Wenhao Ge,Yincong Zhou,Fang Ye,Mengmeng Jiang,Junqing Wu,Yanyu Xiao,Xiaoning Jia,Tingyue Zhang,Xiaojie Ma,Qi Zhang,Xueli Bai,Shujing Lai,Chengxuan Yu,Lijun Zhu,Rui Lin,Yuchi Gao,Min Wang,Yiqing Wu,Jianming Zhang,Renya Zhan,Saiyong Zhu,Hailan Hu,Changchun Wang,Ming Chen,He Huang,Tingbo Liang,Jianghua Chen,Weilin Wang,Dan Zhang,Guoji Guo +38 more
TL;DR: The results provide a useful resource for the study of human biology and find that stem and progenitor cells exhibit strong transcriptomic stochasticity, whereas differentiated cells are more distinct.
Journal ArticleDOI
Effects of COVID-19 on the Nervous System.
TL;DR: A critical appraisal of the potential for neurotropism and mechanisms of neuropathogenesis of SARS-CoV-2, as they relate to the acute and chronic neurological consequences of the infection is provided.
Journal ArticleDOI
Spatial Transcriptomics and In Situ Sequencing to Study Alzheimer's Disease.
Wei Ting Chen,Wei Ting Chen,Ashley Lu,Ashley Lu,Katleen Craessaerts,Katleen Craessaerts,Benjamin Pavie,Carlo Sala Frigerio,Carlo Sala Frigerio,Carlo Sala Frigerio,Nikky Corthout,Xiaoyan Qian,Jana Lalakova,Malte Kühnemund,Iryna Voytyuk,Iryna Voytyuk,Leen Wolfs,Leen Wolfs,Renzo Mancuso,Renzo Mancuso,Evgenia Salta,Evgenia Salta,Sriram Balusu,Sriram Balusu,An Snellinx,An Snellinx,Sebastian Munck,Aleksandra Jurek,José Fernández Navarro,Takaomi C. Saido,Inge Huitinga,Inge Huitinga,Joakim Lundeberg,Mark Fiers,Mark Fiers,Mark Fiers,Bart De Strooper,Bart De Strooper,Bart De Strooper +38 more
TL;DR: Genome-wide spatial transcriptomics analysis provides an unprecedented approach to untangle the dysregulated cellular network in the vicinity of pathogenic hallmarks of AD and other brain diseases.
Journal ArticleDOI
An atlas of the protein-coding genes in the human, pig, and mouse brain
Evelina Sjöstedt,Evelina Sjöstedt,Wen Zhong,Linn Fagerberg,Max J. Karlsson,Nicholas Mitsios,Csaba Adori,Per Oksvold,Fredrik Edfors,Agnieszka Limiszewska,Feria Hikmet,Jinrong Huang,Yutao Du,Lin Lin,Zhanying Dong,Ling Yang,Xin Liu,Hui Jiang,Xun Xu,Jian Wang,Huanming Yang,Lars Bolund,Adil Mardinoglu,Cheng Zhang,Kalle von Feilitzen,Cecilia Lindskog,Fredrik Pontén,Yonglun Luo,Tomas Hökfelt,Mathias Uhlén,Mathias Uhlén,Jan Mulder +31 more
TL;DR: A comprehensive molecular dissection of the main regions of the human, pig, and mouse brain using transcriptomics and antibody-based mapping suggests similar regional organization and expression patterns in the three mammalian species, consistent with the view that basic brain architecture is preserved during mammalian evolution.
Journal ArticleDOI
A single-cell type transcriptomics map of human tissues.
Max J. Karlsson,Cheng Zhang,Loren Méar,Wen Zhong,Andreas Digre,Borbala Katona,Evelina Sjöstedt,Lynn M. Butler,Jacob Odeberg,Philip Dusart,Fredrik Edfors,Per Oksvold,Kalle von Feilitzen,Martin Zwahlen,Muhammad Arif,Ozlem Altay,Xiangyu Li,Mehmet Ozcan,Adil Mardinoglu,Linn Fagerberg,Jan Mulder,Yonglun Luo,Fredrik Pontén,Mathias Uhlén,Mathias Uhlén,Cecilia Lindskog +25 more
TL;DR: In this article, the authors combined single-cell transcriptomics analysis with spatial antibody-based protein profiling to create a high-resolution singlecell type map of human tissues, which was used to explore the expression of human protein-coding genes in 192 individual cell type clusters.
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