The thiol pool in human plasma: the central contribution of albumin to redox processes.
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TLDR
A critical review of the plasma thiol pool is provided with a focus on human serum albumin, an important target for oxidants and electrophiles due to its reactivity with a wide variety of species and its relatively high concentration.About:
This article is published in Free Radical Biology and Medicine.The article was published on 2013-12-01 and is currently open access. It has received 519 citations till now. The article focuses on the topics: Human serum albumin & Thiol.read more
Citations
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A novel and automated assay for thiol/disulphide homeostasis.
Ozcan Erel,Salim Neselioglu +1 more
TL;DR: In this paper, the authors developed a novel and automated assay determining plasma thiol/disulphide homeostasis, which consists of thiol-disoulphide exchanges.
Journal ArticleDOI
Existing and potential therapeutic uses for N-acetylcysteine: the need for conversion to intracellular glutathione for antioxidant benefits.
TL;DR: A key conclusion is a reinforcement of the concept that NAC should not be considered to be a powerful antioxidant in its own right: its strength is the targeted replenishment of GSH in deficient cells and it is likely to be ineffective in cells replete in GSH.
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N-Acetylcysteine as an antioxidant and disulphide breaking agent: the reasons why
Giancarlo Aldini,Alessandra Altomare,Giovanna Baron,Giulio Vistoli,Marina Carini,Luisa Borsani,Francesco Sergio +6 more
TL;DR: The disulphide breaking activity of NAC also explains its mucolytic activity which is due to its effect in reducing heavily cross-linked mucus glycoproteins and free thiols as well as reduced proteins which have important direct antioxidant activity.
Journal ArticleDOI
Peptide and protein nanoparticle conjugates: versatile platforms for biomedical applications
TL;DR: A comprehensive overview of the key sequences and structures utilised to provide biological and physical stability to nano-constructs, direct particles to their target and influence their cellular and tissue distribution, induce and control biological responses, and form polypeptide self-assembled nanoparticles are provided.
Journal ArticleDOI
The Reactive Species Interactome: Evolutionary Emergence, Biological Significance, and Opportunities for Redox Metabolomics and Personalized Medicine.
Miriam M. Cortese-Krott,Anne M. Koning,Gunter G. C. Kuhnle,Péter Nagy,Christopher L. Bianco,Christopher L. Bianco,Andreas Pasch,David A. Wink,Jon M. Fukuto,Jon M. Fukuto,Jon M. Fukuto,Alan Jackson,Harry van Goor,Kenneth R. Olson,Martin Feelisch +14 more
TL;DR: This work introduces a novel integrative concept defined as the reactive species interactome (RSI), a primeval multilevel redox regulatory system whose architecture allows efficient sensing and rapid adaptation to environmental changes and various other stressors to enhance fitness and resilience at the local and whole-organism level.
References
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Crystal structure of human serum albumin at 2.5 A resolution.
TL;DR: A new triclinic crystal form of human serum albumin (HSA), derived either from pool plasma or from a Pichia pastoris expression system, was obtained from polyethylene glycol 4000 solution, and three-dimensional structures of pHSA and rHSA were determined.
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Redefining oxidative stress.
TL;DR: From a mechanistic standpoint, oxidative stress may be better defined as a disruption of redox signaling and control and adoption of such a definition could redirect research to identify key perturbations of red Oxidative stress-related disease processes and lead to new treatments for oxidative stressed disease processes.
Book ChapterDOI
Measurement of protein thiol groups and glutathione in plasma
TL;DR: This chapter describes convenient assays for P-SH and GSH in plasma using spectrophotometric and spectrofluorometric methods.
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Crystallographic Analysis Reveals Common Modes of Binding of Medium and Long-Chain Fatty Acids to Human Serum Albumin
TL;DR: A crystallographic study of five HSA-fatty acid complexes formed using saturated medium-chain and long-chain fatty acids reveals key similarities and significant differences in the modes of binding, and serves to rationalise much of the biochemical data on fatty acid interactions with albumin.
Journal ArticleDOI
Real-time imaging of the intracellular glutathione redox potential.
Marcus Gutscher,Anne Laure Pauleau,Laurent Marty,Thorsten Brach,Guido H. Wabnitz,Yvonne Samstag,Andreas J. Meyer,Tobias P. Dick +7 more
TL;DR: It is demonstrated that the fusion of human glutaredoxin-1 to roGFP2 facilitates specific real-time equilibration between the sensor protein and the glutathione redox couple, which facilitated the observation of redox changes associated with growth factor availability, cell density, mitochondrial depolarization, respiratory burst activity and immune receptor stimulation.