The thiol pool in human plasma: the central contribution of albumin to redox processes.
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TLDR
A critical review of the plasma thiol pool is provided with a focus on human serum albumin, an important target for oxidants and electrophiles due to its reactivity with a wide variety of species and its relatively high concentration.About:
This article is published in Free Radical Biology and Medicine.The article was published on 2013-12-01 and is currently open access. It has received 519 citations till now. The article focuses on the topics: Human serum albumin & Thiol.read more
Citations
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A novel and automated assay for thiol/disulphide homeostasis.
Ozcan Erel,Salim Neselioglu +1 more
TL;DR: In this paper, the authors developed a novel and automated assay determining plasma thiol/disulphide homeostasis, which consists of thiol-disoulphide exchanges.
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Existing and potential therapeutic uses for N-acetylcysteine: the need for conversion to intracellular glutathione for antioxidant benefits.
TL;DR: A key conclusion is a reinforcement of the concept that NAC should not be considered to be a powerful antioxidant in its own right: its strength is the targeted replenishment of GSH in deficient cells and it is likely to be ineffective in cells replete in GSH.
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N-Acetylcysteine as an antioxidant and disulphide breaking agent: the reasons why
Giancarlo Aldini,Alessandra Altomare,Giovanna Baron,Giulio Vistoli,Marina Carini,Luisa Borsani,Francesco Sergio +6 more
TL;DR: The disulphide breaking activity of NAC also explains its mucolytic activity which is due to its effect in reducing heavily cross-linked mucus glycoproteins and free thiols as well as reduced proteins which have important direct antioxidant activity.
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Peptide and protein nanoparticle conjugates: versatile platforms for biomedical applications
TL;DR: A comprehensive overview of the key sequences and structures utilised to provide biological and physical stability to nano-constructs, direct particles to their target and influence their cellular and tissue distribution, induce and control biological responses, and form polypeptide self-assembled nanoparticles are provided.
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The Reactive Species Interactome: Evolutionary Emergence, Biological Significance, and Opportunities for Redox Metabolomics and Personalized Medicine.
Miriam M. Cortese-Krott,Anne M. Koning,Gunter G. C. Kuhnle,Péter Nagy,Christopher L. Bianco,Christopher L. Bianco,Andreas Pasch,David A. Wink,Jon M. Fukuto,Jon M. Fukuto,Jon M. Fukuto,Alan Jackson,Harry van Goor,Kenneth R. Olson,Martin Feelisch +14 more
TL;DR: This work introduces a novel integrative concept defined as the reactive species interactome (RSI), a primeval multilevel redox regulatory system whose architecture allows efficient sensing and rapid adaptation to environmental changes and various other stressors to enhance fitness and resilience at the local and whole-organism level.
References
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Homocysteine and OtherThiolsDeterminedin Plasmaby HPLCand Thiol-Specific Postcolumn Derivatization
TL;DR: The assay utilizes isocratic reversed-phase ion-pair liquid chromatography at pH 2.4, postcolumn derivatization with 4,4'-dithiodipyridine, and colorimetric detection at 324 nm for total and reduced homocysteine.
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Site-specific modification of albumin by free radicals. Reaction with copper(II) and ascorbate.
TL;DR: It appears that Cu(II) bound to the tightest albumin-binding site is less available to the ascorbate than the more loosely bound cation, which generates OH radicals, which rapidly inter-react with the protein and modify it in a 'site-specific' manner.
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Rates of thiol-disulfide interchange reactions between mono- and dithiols and Ellman's reagent
TL;DR: The rate constants for thiol-disulfide interchange between 21 mono- and dithiols and Ellman's reagent correlate with the pKu's of the thiol groups with a Bronsted coefficient of p = 0.36, and a convenient synthesis of a mixture of diastereomers of 1,4-dimercapto-2,3-butanediol has been developed.
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Simultaneous determination of total homocysteine, cysteine, cysteinylglycine, and glutathione in human plasma by high-performance liquid chromatography: application to studies of oxidative stress.
TL;DR: This HPLC method is well suited for high-throughput quantitative determination of aminothiols in clinical studies, and is currently being used to support investigations of oxidative stress in patients with chronic kidney disease.
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Oxidative stress is enhanced in correlation with renal dysfunction: Examination with the redox state of albumin
Hiroyuki Terawaki,Kazunobu Yoshimura,Toshio Hasegawa,Yukie Matsuyama,Tsuneo Negawa,Kenichi Yamada,Masato Matsushima,Masaaki Nakayama,Tatsuo Hosoya,Seiichi Era +9 more
TL;DR: Investigating the current status of oxidative stress in predialysis patients with renal failure found that even before dialysis, oxidative stress was enhanced in correlation with the level of renal dysfunction among patients with chronic renal failure.