Toward the promise of microRNAs - Enhancing reproducibility and rigor in microRNA research.
TLDR
This review is focused on increasing awareness of the challenges of working with microRNAs in translational research and recommends better practices in this area of discovery.Abstract:
The fields of applied and translational microRNA research have exploded in recent years as microRNAs have been implicated across a spectrum of diseases. MicroRNA biomarkers, microRNA therapeutics, microRNA regulation of cellular physiology and even xenomiRs have stimulated great interest, which have brought many researchers into the field. Despite many successes in determining general mechanisms of microRNA generation and function, the application of microRNAs in translational areas has not had as much success. It has been a challenge to localize microRNAs to a given cell type within tissues and assay them reliably. At supraphysiologic levels, microRNAs may regulate hosts of genes that are not the physiologic biochemical targets. Thus the applied and translational microRNA literature is filled with pitfalls and claims that are neither scientifically rigorous nor reproducible. This review is focused on increasing awareness of the challenges of working with microRNAs in translational research and re...read more
Citations
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Journal ArticleDOI
Toward the human cellular microRNAome
Matthew N. McCall,Min-Sik Kim,Min-Sik Kim,Mohammed Adil,Arun H. Patil,Yin Lu,Chris J. Mitchell,Pamela Leal-Rojas,Jinchong Xu,Manoj Kumar,Valina L. Dawson,Ted M. Dawson,Alexander S. Baras,Avi Z. Rosenberg,Dan E. Arking,Kathleen H. Burns,Akhilesh Pandey,Marc K. Halushka +17 more
TL;DR: This project evaluated 8 billion small RNA-seq reads and identified both specific and ubiquitous patterns of expression that strongly correlate with adjacent superenhancer activity, establishing the landscape of human cell-specific microRNA expression.
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Senescence cell–associated extracellular vesicles serve as osteoarthritis disease and therapeutic markers
Ok-Hee Jeon,David R. Wilson,Cristina C. Clement,Sona Rathod,Christopher Cherry,Bonita H. Powell,Zhenghong Lee,Ahmad M. Khalil,Jordan J. Green,Judith Campisi,Judith Campisi,Laura Santambrogio,Kenneth W. Witwer,Jennifer H. Elisseeff +13 more
TL;DR: In this paper, Extracellular Vesicles (EVs) are found to transfer senescence to nonsenescent chondrocytes and inhibited cartilage formation by non-SnCs.
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Circulating miRNA analysis for cancer diagnostics and therapy.
TL;DR: This review provides a comprehensive overview of circulating miRNA analysis and highlights the importance of sampling and quality control, the technical aspects of miRNA extraction and quantification, recommendations for downstream analysis and conclude with future perspectives.
Journal ArticleDOI
Combinatorial Targeting by MicroRNAs Co-ordinates Post-transcriptional Control of EMT.
Joseph Cursons,Katherine A. Pillman,Kaitlin G. Scheer,Philip A. Gregory,Philip A. Gregory,Momeneh Foroutan,Soroor Hediyeh-Zadeh,John Toubia,Edmund J. Crampin,Gregory J. Goodall,Gregory J. Goodall,Cameron P. Bracken,Cameron P. Bracken,Melissa J. Davis,Melissa J. Davis +14 more
TL;DR: Investigating a human mammary cell model of EMT found evidence that a set of miRNAs, including the miR-200 and miR-182/183 family members, co-operate in post-transcriptional regulation, both reinforcing and buffering transcriptional output, and demonstrates that combinatorial treatment altered cellular phenotype with miRNA concentrations much closer to endogenous levels and with less off-target effects.
Journal ArticleDOI
Milk MicroRNAs in Health and Disease
TL;DR: A comprehensive critical review of the key findings surrounding milk microRNAs in human, cow, and goat milk among other species and critically discusses the limitations and outline future aspects and avenues to explore in this rapidly growing field of research.
References
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