Fundación Instituto Leloir

About: Fundación Instituto Leloir is a facility organization based out in Buenos Aires, Argentina. It is known for research contribution in the topics: Dentate gyrus & Neurogenesis. The organization has 702 authors who have published 1052 publications receiving 39299 citations.

ASpli2: Integrative analysis of splicing landscapes through RNA-Seq assays

Abstract: Genome-wide analysis of alternative splicing has been a very active field of research since the early days of NGS (Next generation sequencing) technologies Since then, ever-growing data availability and the development of increasingly sophisticated analysis methods have uncovered the complexity of the general splicing repertoire However, independently of the considered quantification methodology, very often changes in variant concentration profiles can be hard to disentangle In order to tackle this problem we present ASpli2, a computational suite implemented in R, that allows the identification of changes in both, annotated and novel alternative splicing events, and can deal with complex experimental designs Our analysis workflow relies on the analysis of differential usage of sub genic features in combination with a junction-based description of local splicing changes Analyzing simulated and real data we found that the consolidation of these signals resulted in a robust proxy of the occurrence of splicing alterations While junction-based signals allowed us to uncover annotated as well and non-annotated events, bin-associated signals notably increased recall capabilities at a very competitive performance in terms of precision

Abstract 3393: Antibody-based array proteomics identify proteins differentially expressed in human metastatic and non-metastatic triple negative breast cancer cells

Abstract: Proceedings: AACR 103rd Annual Meeting 2012‐‐ Mar 31‐Apr 4, 2012; Chicago, IL Triple negative breast cancers (TNBC) lacking hormone receptors (ER, PR) and HER-2 amplification are very aggressive tumors. The IIB-BR-G cell line isolated from a primary TNBC, and its spontaneous metastatic variant, IIB-BR-G-MTS6 were compared using an antibody-based protein array to characterize their expression profile. We also analyzed their growth kinetics, migration, invasiveness, lymphangiogenesis and cytoskeleton structure. Doubling times in vitro were shorter for IIB-BR-G although in vivo IIB-BR-G-MTS6 tumors grew significantly faster than IIB-BR-G. IIB-BR-GMTS6 showed higher anchorage independent growth in a clonogenic assay. IIB-BR-G-MTS6 cells showed 100% incidence of lymph node metastasis at 5-6 weeks, although no metastasis was observed for IIB-BR-G even 19 weeks after inoculation. CCL3, IL1α, CXCL1, CSF2, CSF3, IGFBP1, IL1α, IL6, IL8, CCL20, PLAUR, PlGF and VEGF were strongly up-regulated in IIB-BR-G-MTS6 compared to IIB-BR-G, while CCL4, ICAM3, CXCL12, TNFRSF18, FIGF, were the more down-regulated proteins in the metastatic cell line. IIB-BR-G-MTS6 protein expression profile was compatible with higher NF-κB activation. In vitro, IIB-BR-G displayed higher migration but IIB-BR-G-MTS6 had a higher matrigel invasion. Accordingly, IIB-BR-G-MTS6 had an up-regulated expression of MMP1, MMP9, MMP13, PLAUR and HGF as compared to IIB-BR-G. IIB-BR-G-MTS6 tumors presented higher local lymphatic invasion than IIB-BR-G but similar lymphatic vessel densities. VEGFC and VEGFA/B expression were higher both in vitro and in vivo for IIB-BR-G-MTS6. Higher vimentin expression was mainly localized in the cellular extremities in IIB-BR-G-MTS6.Our results suggest that IIB-BR-G-MTS6 have acquired an epithelial-to-mesenchymal transition phenotype, modulating tumor stroma to favor tumor growth, increase angiogenesis and promote their metastatic dissemination. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 103rd Annual Meeting of the American Association for Cancer Research; 2012 Mar 31-Apr 4; Chicago, IL. Philadelphia (PA): AACR; Cancer Res 2012;72(8 Suppl):Abstract nr 3393. doi:1538-7445.AM2012-3393

Production of a Highly Immunogenic Antigen from SARS-CoV-2 by Covalent Coupling of the Receptor Binding Domain of Spike Protein to a Multimeric Carrier

Abstract: Fil: Argentinian AntiCovid Consortium. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Parque Centenario. Instituto de Investigaciones Bioquimicas de Buenos Aires. Fundacion Instituto Leloir. Instituto de Investigaciones Bioquimicas de Buenos Aires; Argentina