Institution
University of Guelph
Education•Guelph, Ontario, Canada•
About: University of Guelph is a education organization based out in Guelph, Ontario, Canada. It is known for research contribution in the topics: Population & Gene. The organization has 26542 authors who have published 50553 publications receiving 1715255 citations. The organization is also known as: U of G & Guelph University.
Topics: Population, Gene, Context (language use), Poison control, Soil water
Papers published on a yearly basis
Papers
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TL;DR: The system-level approach to study the interactions between the gut microbiota and host in new-onset paediatric patients reveals that host–microbiota interactions are disturbed in CD and thus provides mechanistic insights into CD pathogenesis.
Abstract: Intestinal microbial dysbiosis is associated with Crohn's disease (CD). However, the mechanisms leading to the chronic mucosal inflammation that characterizes this disease remain unclear. In this report, we use systems-level approaches to study the interactions between the gut microbiota and host in new-onset paediatric patients to evaluate causality and mechanisms of disease. We report an altered host proteome in CD patients indicative of impaired mitochondrial functions. In particular, mitochondrial proteins implicated in H2S detoxification are downregulated, while the relative abundance of H2S microbial producers is increased. Network correlation analysis reveals that Atopobium parvulum controls the central hub of H2S producers. A. parvulum induces pancolitis in colitis-susceptible interleukin-10-deficient mice and this phenotype requires the presence of the intestinal microbiota. Administrating the H2S scavenger bismuth mitigates A. parvulum-induced colitis in vivo. This study reveals that host-microbiota interactions are disturbed in CD and thus provides mechanistic insights into CD pathogenesis.
267 citations
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TL;DR: The results obtained in this study indicate that lysozyme possesses nonenzymatic bacteriostatic domains in its primary sequence and they are released by proteolytic hydrolysis.
Abstract: This work was aimed at the isolation, purification, and characterization of novel antimicrobial peptides from chicken egg white lysozyme hydrolysate, obtained by peptic digestion and subsequent tryptic digestion. The hydrolysate was composed of over 20 small peptides of less than 1000 Da, and had no enzymatic activity. The water-soluble peptide mixture showed bacteriostatic activity against Gram-positive bacteria (Staphylococcus aureus 23-394) and Gram-negative bacteria (Escherichia coli K-12). Two bacteriostatic peptides were purified and sequenced. One peptide, with the sequence Ile-Val-Ser-Asp-Gly-Asp-Gly-Met-Asn-Ala-Trp, inhibited Gram-negative bacteria E. coli K-12 and corresponded to amino acid residues 98-108, which are located in the middle part of the helix-loop-helix. Another novel antimicrobial peptide inhibited S. aureus 23-394 and was determined to have the sequence His-Gly-Leu-Asp-Asn-Tyr-Arg, corresponding to amino acid residues 15-21 of lysozyme. These peptides broadened the antimicrobial activity of lysozyme to include Gram-negative bacteria. The results obtained in this study indicate that lysozyme possesses nonenzymatic bacteriostatic domains in its primary sequence and they are released by proteolytic hydrolysis.
267 citations
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TL;DR: A role for FAT/CD36 in mitochondrial long chain fatty acid uptake is suggested and system flexibility is demonstrated to match FAT/ CD36 mitochondrial content with an increased capacity for fatty acid oxidation, possibly involving translocation of FAT/cd36 to the mitochondria.
267 citations
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TL;DR: In this chapter, the protocols involved in the assembly of DNA barcode records for members of the animal kingdom are detailed, but many of these approaches are of more general application.
Abstract: The Barcode of Life initiative represents an ambitious effort to develop an identification system for eukaryotic life based on the analysis of sequence diversity in short, standardized gene regions. Work is furthest advanced for members of the animal kingdom. In this case, a target gene region has been selected (cytochrome c oxidase I) and pilot studies have validated its effectiveness in species discovery and identification. Based on these positive results, there is now a growing effort to both gather barcode records on a large-scale for members of this kingdom and to identify target barcode regions for the other kingdoms of eukaryotes. In this chapter, we detail the protocols involved in the assembly of DNA barcode records for members of the animal kingdom, but many of these approaches are of more general application.
267 citations
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Xishuangbanna Tropical Botanical Garden1, Boston University2, University of Montpellier3, University of Vienna4, Memorial University of Newfoundland5, National University of Singapore6, Arthur Rylah Institute for Environmental Research7, James Hutton Institute8, James Cook University9, University of Wisconsin-Madison10, Portland State University11, University of Guelph12
TL;DR: This editorial can only be a snapshot of a quickly evolving situation, but it hopes that it can offer some encouragement and insights for colleagues in lockdown and how the conservation community must be ready to respond.
267 citations
Authors
Showing all 26778 results
Name | H-index | Papers | Citations |
---|---|---|---|
Dirk Inzé | 149 | 647 | 74468 |
Norbert Perrimon | 138 | 610 | 73505 |
Bobby Samir Acharya | 133 | 1121 | 100545 |
Eduardo Marbán | 129 | 579 | 49586 |
Benoît Roux | 120 | 493 | 62215 |
Fereidoon Shahidi | 119 | 951 | 57796 |
Stephen Safe | 116 | 784 | 60588 |
Mark A. Tarnopolsky | 115 | 644 | 42501 |
Robert C. Haddon | 112 | 577 | 52712 |
Milton H. Saier | 111 | 707 | 54496 |
Hans J. Vogel | 111 | 1260 | 62846 |
Paul D. N. Hebert | 111 | 537 | 66288 |
Peter T. Katzmarzyk | 110 | 618 | 56484 |
John Campbell | 107 | 1150 | 56067 |
Linda F. Nazar | 106 | 318 | 52092 |