Characterization of microRNAs in serum: a novel class of biomarkers for diagnosis of cancer and other diseases
Xi Chen,Yi Ba,Lijia Ma,Lijia Ma,Xing Cai,Yuan Yin,Kehui Wang,Jigang Guo,Yujing Zhang,Jiangning Chen,Xing Guo,Qibin Li,Qibin Li,Xiaoying Li,Wenjing Wang,Yan Zhang,Jin Wang,Xueyuan Jiang,Yang Xiang,Chen Xu,Pingping Zheng,Juanbin Zhang,Ruiqiang Li,Hongjie Zhang,Xiaobin Shang,Ting Gong,Guang Ning,Jun Wang,Jun Wang,Ke Zen,Junfeng Zhang,Chen-Yu Zhang +31 more
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TLDR
It is demonstrated that miRNAs are present in the serum and plasma of humans and other animals such as mice, rats, bovine fetuses, calves, and horses, and can serve as potential biomarkers for the detection of various cancers and other diseases.Abstract:
Dysregulated expression of microRNAs (miRNAs) in various tissues has been associated with a variety of diseases, including cancers. Here we demonstrate that miRNAs are present in the serum and plasma of humans and other animals such as mice, rats, bovine fetuses, calves, and horses. The levels of miRNAs in serum are stable, reproducible, and consistent among individuals of the same species. Employing Solexa, we sequenced all serum miRNAs of healthy Chinese subjects and found over 100 and 91 serum miRNAs in male and female subjects, respectively. We also identified specific expression patterns of serum miRNAs for lung cancer, colorectal cancer, and diabetes, providing evidence that serum miRNAs contain fingerprints for various diseases. Two non-small cell lung cancer-specific serum miRNAs obtained by Solexa were further validated in an independent trial of 75 healthy donors and 152 cancer patients, using quantitative reverse transcription polymerase chain reaction assays. Through these analyses, we conclude that serum miRNAs can serve as potential biomarkers for the detection of various cancers and other diseases.read more
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Altered Circulating miRNA Expression Profile in Pregestational and Gestational Obesity
Gemma Carreras-Badosa,Alexandra Bonmati,F Ortega,Josep-Maria Mercader,Marta Guindo-Martínez,David Torrents,Anna Prats-Puig,Jose-Maria Martinez-Calcerrada,Estibaliz Platero-Gutierrez,Francis de Zegher,Lourdes Ibáñez,José Manuel Fernández-Real,Abel López-Bermejo,Judit Bassols +13 more
TL;DR: This study provides the first identification of altered circulating miRNAs in maternal obesity and suggests a possible role of such miRNAS as markers for pre- and postnatal growth.
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Exosomal miRNAs in hepatocellular carcinoma development and clinical responses.
TL;DR: Evidence indicates that exosomal miRNAs in exosomes can not only serve as sensitive biomarkers for cancer diagnostics and recurrence but can also potentially be used as therapeutics to target HCC progression.
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Profiling Circulating MicroRNA Expression in Experimental Sepsis Using Cecal Ligation and Puncture
Shao-Chun Wu,Johnson Chia-Shen Yang,Cheng-Shyuan Rau,Yi-Chun Chen,Tsu-Hsiang Lu,Ming-Wei Lin,Siou-Ling Tzeng,Yi-Chan Wu,Chia-Jung Wu,Ching-Hua Hsieh +9 more
TL;DR: This study identified circulating miRNAs that were up-regulated after CLP and determined the increase in the levels of these miRNAAs, and the results suggest that circulating Ago2 complexes and exosomes may be responsible for the stability of mi RNAs in the serum.
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Nitrogen-doped hollow carbon nanospheres for high-energy-density biofuel cells and self-powered sensing of microRNA-21 and microRNA-141
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MicroRNAs as potential liquid biopsy biomarkers in colorectal cancer: A systematic review.
TL;DR: The field of noncoding RNAs, particularly for the clinical use of miRNAs as liquid biopsy assays is maturing rapidly, and it is highly promising that these genomic signatures will likely be developed into clinically-viable tests for the early detection and clinical management of patients with colorectal cancer in the not so distant future.
References
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Circulating microRNAs as stable blood-based markers for cancer detection
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Caifu Chen,Dana Ridzon,Adam Broomer,Zhaohui Zhou,Danny H. Lee,Julie T. Nguyen,Maura Barbisin,Nan Lan Xu,Vikram R. Mahuvakar,Mark R. Andersen,Kaiqin Lao,Kenneth J. Livak,Karl J. Guegler +12 more
TL;DR: A novel microRNA quantification method has been developed using stem–loop RT followed by TaqMan PCR analysis, which enables fast, accurate and sensitive miRNA expression profiling and can identify and monitor potential biomarkers specific to tissues or diseases.
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