Characterization of microRNAs in serum: a novel class of biomarkers for diagnosis of cancer and other diseases
Xi Chen,Yi Ba,Lijia Ma,Lijia Ma,Xing Cai,Yuan Yin,Kehui Wang,Jigang Guo,Yujing Zhang,Jiangning Chen,Xing Guo,Qibin Li,Qibin Li,Xiaoying Li,Wenjing Wang,Yan Zhang,Jin Wang,Xueyuan Jiang,Yang Xiang,Chen Xu,Pingping Zheng,Juanbin Zhang,Ruiqiang Li,Hongjie Zhang,Xiaobin Shang,Ting Gong,Guang Ning,Jun Wang,Jun Wang,Ke Zen,Junfeng Zhang,Chen-Yu Zhang +31 more
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TLDR
It is demonstrated that miRNAs are present in the serum and plasma of humans and other animals such as mice, rats, bovine fetuses, calves, and horses, and can serve as potential biomarkers for the detection of various cancers and other diseases.Abstract:
Dysregulated expression of microRNAs (miRNAs) in various tissues has been associated with a variety of diseases, including cancers. Here we demonstrate that miRNAs are present in the serum and plasma of humans and other animals such as mice, rats, bovine fetuses, calves, and horses. The levels of miRNAs in serum are stable, reproducible, and consistent among individuals of the same species. Employing Solexa, we sequenced all serum miRNAs of healthy Chinese subjects and found over 100 and 91 serum miRNAs in male and female subjects, respectively. We also identified specific expression patterns of serum miRNAs for lung cancer, colorectal cancer, and diabetes, providing evidence that serum miRNAs contain fingerprints for various diseases. Two non-small cell lung cancer-specific serum miRNAs obtained by Solexa were further validated in an independent trial of 75 healthy donors and 152 cancer patients, using quantitative reverse transcription polymerase chain reaction assays. Through these analyses, we conclude that serum miRNAs can serve as potential biomarkers for the detection of various cancers and other diseases.read more
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miR-125a-5p is a prognostic biomarker that targets HDAC4 to suppress breast tumorigenesis
Tsung-Hua Hsieh,Chia-Yi Hsu,Cheng-Fang Tsai,Cheng-Yu Long,Chee-Yin Chai,Ming-Feng Hou,Jau-Nan Lee,Deng-Chyang Wu,Shao Chun Wang,Eing-Mei Tsai +9 more
TL;DR: It is demonstrated that serum miR-125a-5p level in breast cancer may be a useful prognostic biomarker and offer a novel therapeutic avenue by targeting HDAC4 in Breast cancer.
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Higher expression of circulating miR-182 as a novel biomarker for breast cancer.
Ping‑Yu Wang,Hai‑Tao Gong,Bao‑Feng Li,Chun‑Lei Lv,Huan‑Tai Wang,Huihui Zhou,Xin-Xin Li,Shu‑Yang Xie,Baofa Jiang +8 more
TL;DR: Results consistent with miR-182 as a novel and valuable biomarker for the diagnosis of BC are highlighted.
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Intracellular modulation, extracellular disposal and serum increase of MiR-150 mark lymphocyte activation.
Paola de Candia,Anna Torri,Tatiana Gorletta,Maya Fedeli,Elisabetta Bulgheroni,Cristina Cheroni,Francesco Marabita,Mariacristina Crosti,Monica Moro,Elena Pariani,Luisa Romanò,Susanna Esposito,Fabio Mosca,Grazisa Rossetti,Riccardo L. Rossi,Jens Geginat,Giulia Casorati,Paolo Dellabona,Massimiliano Pagani,Sergio Abrignani +19 more
TL;DR: It is found that, upon activation in vitro, human and mouse lymphocytes down-modulate intracellular miR-150 and accumulate it in exosomes, which suggests that the selective extracellular disposal of microRNAs can be a rapid way to regulate gene expression during lymphocyte activation.
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A combination of circulating miRNAs for the early detection of ovarian cancer
Akira Yokoi,Akira Yokoi,Yusuke Yoshioka,Akihiro Hirakawa,Yusuke Yamamoto,Mitsuya Ishikawa,Shun ichi Ikeda,Tomoyasu Kato,Kaoru Niimi,Hiroaki Kajiyama,Fumitaka Kikkawa,Takahiro Ochiya +11 more
TL;DR: This method was able to successfully distinguish ovarian cancer patients from healthy controls and early-stage ovarian cancer from patients with benign tumors, and enables subtype classification in 4 types of epithelial ovarian cancer.
Journal ArticleDOI
Serum microRNAs as novel biomarkers for primary sclerosing cholangitis and cholangiocarcinoma
Francesca Bernuzzi,Francesco Marabita,Ana Lleo,Marco Carbone,M. Mirolo,Marco Marzioni,Gianfranco Alpini,Domenico Alvaro,Kirsten Muri Boberg,Massimo Locati,Guido Torzilli,Lorenza Rimassa,Fabio Piscaglia,Xiao-Song He,Christopher L. Bowlus,G.-X. Yang,M. E. Gershwin,Pietro Invernizzi +17 more
TL;DR: This study provides a basis for the use of miRNAs as biomarkers for the diagnosis of PSC and CC and demonstrates a difference in the expression of miR‐222 and miR-483‐5p in CC versus PSC.
References
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TL;DR: A novel microRNA quantification method has been developed using stem–loop RT followed by TaqMan PCR analysis, which enables fast, accurate and sensitive miRNA expression profiling and can identify and monitor potential biomarkers specific to tissues or diseases.
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