Characterization of microRNAs in serum: a novel class of biomarkers for diagnosis of cancer and other diseases
Xi Chen,Yi Ba,Lijia Ma,Lijia Ma,Xing Cai,Yuan Yin,Kehui Wang,Jigang Guo,Yujing Zhang,Jiangning Chen,Xing Guo,Qibin Li,Qibin Li,Xiaoying Li,Wenjing Wang,Yan Zhang,Jin Wang,Xueyuan Jiang,Yang Xiang,Chen Xu,Pingping Zheng,Juanbin Zhang,Ruiqiang Li,Hongjie Zhang,Xiaobin Shang,Ting Gong,Guang Ning,Jun Wang,Jun Wang,Ke Zen,Junfeng Zhang,Chen-Yu Zhang +31 more
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TLDR
It is demonstrated that miRNAs are present in the serum and plasma of humans and other animals such as mice, rats, bovine fetuses, calves, and horses, and can serve as potential biomarkers for the detection of various cancers and other diseases.Abstract:
Dysregulated expression of microRNAs (miRNAs) in various tissues has been associated with a variety of diseases, including cancers. Here we demonstrate that miRNAs are present in the serum and plasma of humans and other animals such as mice, rats, bovine fetuses, calves, and horses. The levels of miRNAs in serum are stable, reproducible, and consistent among individuals of the same species. Employing Solexa, we sequenced all serum miRNAs of healthy Chinese subjects and found over 100 and 91 serum miRNAs in male and female subjects, respectively. We also identified specific expression patterns of serum miRNAs for lung cancer, colorectal cancer, and diabetes, providing evidence that serum miRNAs contain fingerprints for various diseases. Two non-small cell lung cancer-specific serum miRNAs obtained by Solexa were further validated in an independent trial of 75 healthy donors and 152 cancer patients, using quantitative reverse transcription polymerase chain reaction assays. Through these analyses, we conclude that serum miRNAs can serve as potential biomarkers for the detection of various cancers and other diseases.read more
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The role of microRNAs in cancer: diagnostic and prognostic biomarkers and targets of therapies
Lidia Rita Corsini,Giuseppe Bronte,Marianna Terrasi,Valeria Amodeo,Daniele Fanale,Eugenio Fiorentino,Giuseppe Cicero,Viviana Bazan,Antonio Russo +8 more
TL;DR: Both miRNA expression profiles in cancer tissues and miRNA levels in peripheral blood were studied for improvement in the management of cancer patients and the potential for better understanding of tumor biology was found.
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MicroRNAs in spent blastocyst culture medium are derived from trophectoderm cells and can be explored for human embryo reproductive competence assessment.
Antonio Capalbo,Filippo Maria Ubaldi,Danilo Cimadomo,Laila Noli,Yakoub Khalaf,Alessio Farcomeni,Dusko Ilic,Laura Rienzi +7 more
TL;DR: MicroRNAs secreted from human blastocysts in culture media can be profiled with high reproducibility, and this approach can be further explored for noninvasive embryo selection.
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MicroRNA-21 in Scleroderma Fibrosis and its Function in TGF-β- Regulated Fibrosis-Related Genes Expression
Honglin Zhu,Hui Luo,Yisha Li,Yaou Zhou,Ying Jiang,Jin Chai,Xianzhong Xiao,Yunhui You,Xiaoxia Zuo +8 more
TL;DR: It is found that TGF-β regulated the expression of miR-21 and fibrosis-related genes, and decreased Smad7 expression, and suggesting that mi R-21 may act as a potential therapeutic target.
Journal ArticleDOI
MicroRNA-423-5p Promotes Autophagy in Cancer Cells and Is Increased in Serum From Hepatocarcinoma Patients Treated With Sorafenib.
Paola Stiuso,Nicoletta Potenza,Angela Lombardi,Ida Ferrandino,Antonio Monaco,Silvia Zappavigna,Daniela Vanacore,Nicola Mosca,Filomena Castiello,Stefania Porto,Raffaele Addeo,Salvatore Del Prete,Ferdinando De Vita,Aniello Russo,Michele Caraglia +14 more
TL;DR: The results suggest the miR423-5p can be used as a useful tool to predict response to sorafenib in HCC patients and is involved in autophagy regulation in H CC cells.
Journal ArticleDOI
Analytical challenges and technical limitations in assessing circulating miRNAs.
Anna Zampetaki,Manuel Mayr +1 more
TL;DR: A variety of technological approaches and platforms have been developed with increased sensitivity and specificity for the detection and quantification of circulating miRNAs and the analytical challenges in profiling circulatingMiRNAs are discussed.
References
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TL;DR: A novel microRNA quantification method has been developed using stem–loop RT followed by TaqMan PCR analysis, which enables fast, accurate and sensitive miRNA expression profiling and can identify and monitor potential biomarkers specific to tissues or diseases.
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